A5037754012- Hearing, Cochlea, Tinnitus, Genetics
- Hearing Loss and Rehabilitation
- Vestibular and auditory disorders
- Neuroscience and Neuropharmacology Research
- Ear Surgery and Otitis Media
- Cellular transport and secretion
- Salivary Gland Tumors Diagnosis and Treatment
- Lipid Membrane Structure and Behavior
- Advanced Fluorescence Microscopy Techniques
- Cancer and Skin Lesions
- Neural dynamics and brain function
- Nasal Surgery and Airway Studies
- Digital Holography and Microscopy
- Tracheal and airway disorders
- Ion channel regulation and function
- Ear and Head Tumors
- Erythrocyte Function and Pathophysiology
- Acoustic Wave Phenomena Research
- Ion Channels and Receptors
- Advanced Electron Microscopy Techniques and Applications
- Peripheral Artery Disease Management
- Obstructive Sleep Apnea Research
- Head and Neck Anomalies
- Venous Thromboembolism Diagnosis and Management
- Cell Image Analysis Techniques
Universitätsmedizin Göttingen
2006-2025
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2020-2024
MSB Medical School Berlin
2023-2024
Helios Hospital Berlin-Buch
2023-2024
University of Kansas Medical Center
2024
Friedrich-Ebert-Stiftung e.V.
2022
University of Göttingen
2007-2021
Krebsgesellschaft Nordrhein-Westfalen
2018
Max Planck Institute for Biophysical Chemistry
1999-2008
The University of Texas Southwestern Medical Center
2008
During synaptic vesicle fusion, the soluble N -ethylmaleimide-sensitive factor–attachment protein receptor (SNARE) syntaxin-1 exhibits two conformations that both bind to Munc18-1: a “closed” conformation outside SNARE complex and an “open” in complex. Although complexes containing open Munc18-1 are essential for exocytosis, function of closed is unknown. We generated knockin/knockout mice expressed only syntaxin-1B. Syntaxin-1B Open were viable but succumbed generalized seizures at 2 3...
At the large excitatory calyx of Held synapse, quantal size during an evoked EPSC and number active zones contributing to transmission are not known. We developed a nonstationary variant fluctuation analysis determine these parameters. AMPA receptor-mediated EPSCs were recorded in slices young (postnatal 8-10 d) rats after afferent fiber stimulation, delivered trains induce synaptic depression. The means variances amplitudes calculated across for each stimulus number. During 10 Hz at 2 mm...
Synaptotagmin 1, a Ca<sup>2+</sup> sensor for fast synaptic vesicle exocytosis, contains two C<sub>2</sub> domains that form Ca<sup>2+</sup>-dependent complexes with phospholipids. To examine the functional importance of binding to C<sub>2</sub>A domain synaptotagmin we studied mutations, D232N and D238N, using recombinant proteins knock-in mice. Both mutations severely decreased intrinsic phospholipid by isolated domain. however, did not alter apparent Ca<sup>2+</sup>affinity double...
Sound coding at the auditory inner hair cell synapse requires graded changes in neurotransmitter release, triggered by sustained activation of presynaptic Ca(v)1.3 voltage-gated Ca(2+) channels. Central to their role this regard, channels cells show little Ca(2+)-dependent inactivation, a fast negative feedback regulation incoming ions, which depends on calmodulin association with channel alpha(1) subunit. inactivation characterizes nearly all including other excitable cells. The mechanism...
Synaptotagmin-1, the Ca 2+ sensor for fast neurotransmitter release, was proposed to function by -dependent phospholipid binding and/or soluble N -ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex binding. Extensive in vivo data support first hypothesis, but testing second hypothesis has been difficult because no synaptotagmin-1 mutation is known that selectively interferes with SNARE Using knock-in mice carry aspartate-to-asparagine substitutions a -binding site of...
Hearing relies on Ca 2+ influx-triggered exocytosis in cochlear inner hair cells (IHCs). Here we studied the role of channel subunit V β 2 hearing. Of 1–4 mRNAs, IHCs predominantly contained . was severely impaired mice lacking extracardiac tissues ( −/− ). This involved deficits amplification and sound encoding. Otoacoustic emissions were reduced or absent mice, which showed strongly elevated auditory thresholds single neuron recordings brainstem response measurements. greatly (by 68%)....
The cochlea of our auditory system is an intricate structure deeply embedded in the temporal bone. Compared with other sensory organs such as eye, has remained poorly accessible for investigation, example, by imaging. This limitation also concerns further development technology restoring hearing case cochlear dysfunction, which requires quantitative information on spatial dimensions and sensorineural status cochlea. Here, we employed X-ray phase-contrast tomography light-sheet fluorescence...
An 8-year follow-up of Ceravital middle ear prostheses showed there was extremely good tolerance in the space. Incompatibility phenomena were not observed, and inflammatory reactions neither caused nor supported by implants. The tympanoplasties always performed without interposition cartilage between tympanic membrane or graft disk-shaped portion implant, extrusions never observed. Long-lasting processes appeared to destroy implants same way they ossicles. Transient periods (such as episodes...
Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem excised non-human primate rodent cochleae at different [Formula: see text]-CT nano-CT synchrotron instruments. We explore sample embeddings, stainings regimes. Under optimized conditions preparation, instrumentation, protocol, retrieval, high image quality detail level can be achieved in 3D...
We demonstrate that phase retrieval and tomographic imaging at the organ level of small animals can be advantageously carried out using monochromatic radiation emitted by a compact x-ray light source, without further optical elements apart from source detector. This approach allows to carry microtomography experiments which - due large performance gap with respect conventional laboratory instruments so far were usually limited synchrotron sources. potential mapping functional soft tissue...
To review the results of obliteration a preexisting mastoid cavity with abdominal fat and Vibrant Soundbridge implantation in patients mixed hearing loss (MHL) to compare data MHL without pure sensorineural (SNHL).Retrospective chart analysis 10 (10 ears) cavity, 18 (19 alone nine SNHL treated one tertiary referral center.Vibrant case existed previously. Pure tone audiometry (average air-bone gap, average functional gain), speech (Freiburg Monosyllabic Test) complication rate were main...
This report presents a case of endotracheal metastasis in which elective veno–venous extracorporeal membrane oxygenation (VV ECMO) was used to undergo tracheal laser-surgery prior establishment definitive airway. Specifically, we describe the respiratory and airway management an adult patient from preclinical phase throughout preoperative ECMO implantation postoperative weaning decannulation Intensive Care Unit. lends further supports idea that could be electively provide safe environment...
Obstructive sleep apnea (OSA) may result in severe health onditions, reduces quality of live, and affects high percentages the adult population. Due to recent changes German care regulations, mandibular advancement devices (MAD) will become available as a treatment option for OSA greater extent general dentists their patients.A guideline development group consisting nine members representing four dental medical organizations was formed, order provide critical information orientation main...
Abstract Optogenetic control of cells is a key life sciences method and promises novel therapies. Here we report on ChReef, an improved variant the channelrhodopsin ChRmine. ChReef offers minimal photocurrent desensitization, unitary conductance 80 fS closing kinetics 30 ms, which together enable reliable optogenetic at low light levels (nano-Joule) with good temporal fidelity allows sustained stimulation. We demonstrate efficient red-light pacing depolarization block ChReef-expressing...
Objective: Loss of spiral ganglion neurons (SGN) is permanent and responsible for a substantial number patients suffering from hearing impairment. It can derive the degeneration SGNs due to death sensory hair cells as well auditory neuropathy. Utilizing stem recover lost increasingly emerges possible therapeutic option, but access human difficult their protected location within bony impacted cochlea. Aim this study was establish reliable practicable approach in temporal bone cell gene...
Removal of extracellular calcium may result in depolarization the resting cell membrane potential. This has been attributed to stabilizing action on ionic permeability membrane. It is unknown whether this phenomenon exclusively mediated by or through associated changes intracellular calcium. To examine this, we exposed rat aortic smooth muscle cells culture different concentrations and studied their effects potential activity. The was dependent potassium concentration. Exposure reduced (0.25...
Blastomyces dermatitidis is an endemic mycosis in certain regions of the United States America and can cause disseminated infection immunocompetent individuals. We present a case osseous blastomycosis previously healthy 22-year-old male from Missouri, who developed sudden onset right knee pain June 2019. Magnetic Resonance Imaging (MRI) demonstrated enhancing mass that was concerning for malignancy. Antigen antibody testing dimorphic fungi confounding. He underwent debridement surgical...