A5028097108- bioluminescence and chemiluminescence research
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Sensors
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Advanced Fluorescence Microscopy Techniques
- Microbial Community Ecology and Physiology
- Gene expression and cancer classification
- Milk Quality and Mastitis in Dairy Cows
- Protein Kinase Regulation and GTPase Signaling
- Pancreatic function and diabetes
- Fungal and yeast genetics research
- CRISPR and Genetic Engineering
- Animal health and immunology
- Microbial Metabolic Engineering and Bioproduction
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
- Cancer Cells and Metastasis
- Congenital Diaphragmatic Hernia Studies
- Biosensors and Analytical Detection
- Diatoms and Algae Research
- Microbial infections and disease research
- Bacteriophages and microbial interactions
The Netherlands Cancer Institute
2021-2025
University of Amsterdam
2020-2025
Delft University of Technology
2014-2024
Wageningen University & Research
2016
The most successful genetically encoded calcium indicators (GECIs) employ an intensity or ratiometric readout. Despite a large calcium-dependent change in fluorescence intensity, the quantification of concentrations with GECIs is problematic, which further complicated by sensitivity all to changes pH biological range. Here, we report on sensing strategy conformational directly modifies quantum yield and lifetime circular permutated turquoise fluorescent protein. absolute parameter that...
Rho GTPases are regulatory proteins, which orchestrate cell features such as morphology, polarity and movement. Therefore, probing GTPase activity is key to understanding processes development migration. Localization-based reporters for active attractive probes study GTPase-mediated in real time with subcellular resolution living cells tissue. Until now, relocation biosensors (sensors that relocalize the native location of GTPase) seem have been only useful certain organisms not...
Fluorescent biosensors toggle between two states and for the vast majority of one state is bright other dim. As a consequence, there substantial difference in signal-to-noise ratio (SNR) states. The dim has low ratio, which problematic when precise, quantitative measurements are needed. During engineering red-shifted variant an mTurquoise-based calcium sensor, we serendipitously generated green-emitting sensor that shows high brightness both calcium-bound -unbound state, while still showing...
Fluorescent biosensors toggle between two states and for the vast majority of one state is bright other dim. As a consequence, there substantial difference in signal-to-noise ratio (SNR) states. The dim has low ratio, which problematic when precise, quantitative measurements are needed. During engineering red-shifted variant an mTurquoise-based calcium sensor, we serendipitously generated green-emitting sensor that shows high brightness both calcium-bound -unbound state, while still showing...
Large strain construction programs and functional analysis studies are becoming commonplace in Saccharomyces cerevisiae involve of strains that carry multiple selectable marker genes. Extensive engineering is, however, severely hampered by the limited number recyclable genes reduced genome stability occurs upon repeated use heterologous recombinase-based removal methods. The present study proposes an efficient method to recycle markers S. simultaneously, thereby circumventing shortcomings...
Natural antibodies (NAbs) are mostly IgM produced without antigenic stimulation and serve as a first line of defence the immune system. As both natural specific present in animals, NAbs studied by determining response to naïve antigens like keyhole limpet hemocyanin (KLH). In this study, we selected cows based on high low anti-KLH titers, reflecting NAb determined if titers were indicative for recognition common microbial structures (lipopolysaccharide, lipoteichoic acid peptidoglycan)...
Abstract Fluorescent biosensors toggle between two states and for the vast majority of one state is bright other dim. As a consequence, there substantial difference in signal-to-noise ratio (SNR) states. The dim has low ratio, which problematic when precise, quantitative measurements are needed. During engineering red-shifted variant an mTurquoise-based calcium sensor, we serendipitously generated green-emitting sensor that shows high brightness both calcium-bound -unbound state, while still...
The past decades, researchers have worked on the development of genetically encoded biosensors, including over 60 calcium indicators (GECIs) containing a single fluorescent protein (FP). Red GECIs provide advantages in terms imaging depths and reduced cell toxicity. Most respond with fluorescence intensity change, strived to improve sensors brightness fold-change. Unfortunately, is influenced by many factors other than desired sensor response. lifetime contrast overcome this drawback, but so...
Abstract Rho GTPases are regulatory proteins, which orchestrate cell features such as morphology, polarity and movement. Therefore, probing GTPase activity is key to understanding processes development, migration wound healing. Localization-based reporters for active attractive probes study GTPase-mediated processes, in real time with subcellular resolution living cells tissue. Until now, relocation RhoA biosensors seem only be useful certain organisms have not been characterized well. In...
Abstract The most successful genetically encoded calcium indicators (GECIs) employ an intensity or intensiometric readout. Despite a large calcium-dependent change in fluorescence intensity, the quantification of concentrations with GECIs is problematic, which further complicated by sensitivity all to changes pH biological range. Here, we report on novel sensing strategy conformational directly modifies quantum yield and lifetime circular permutated turquoise fluorescent protein. absolute...