One of the impediments to treatment brain tumors (e.g., gliomas) has been degree which they expand, infiltrate surrounding tissue, and migrate widely into normal brain, usually rendering them “elusive” effective resection, irradiation, chemotherapy, or gene therapy. We demonstrate that neural stem cells (NSCs), when implanted experimental intracranial gliomas in vivo adult rodents, distribute themselves quickly extensively throughout tumor bed uniquely juxtaposition expanding aggressively...

The transplantation of neural stem cells (NSCs) offers a new potential therapeutic approach as cell-based delivery system for gene therapy in brain tumors. This is based on the unique capacity NSCs to migrate throughout and target invading tumor cells. However, signals controlling targeted migration transplanted are poorly defined. We analyzed vitro vivo effects angiogenic growth factors protein extracts from surgical specimens patients NSC migration. Here, we demonstrate that vascular...

Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease of the motor system. Recent work in rodent models ALS has shown that insulin-like growth factor-1 (IGF-1) slows progression when delivered at onset. However, IGF-1's mechanism action along neuromuscular axis remains unclear. In this study, symptomatic mice received IGF-1 through stereotaxic injection an IGF-1-expressing viral vector to deep cerebellar nuclei (DCN), region cerebellum with extensive brain stem and spinal...

The aims of this study are to demonstrate the increased lysis stem cells but not their differentiated counterparts by NK and determine whether disturbance in cell differentiation is a cause for sensitivity mediated cytotoxicity. Increased cytotoxicity augmented secretion IFN-gamma were both observed when PBMCs or co-incubated with primary UCLA oral squamous carcinoma (UCLA-OSCSCs) compared (UCLA-OSCCs). In addition, human embryonic (hESCs) also lysed greatly cells. Moreover, found lyse...

The remarkable migratory and tumor-tropic capacities of neural stem cells (NSCs and/or neuroprogenitor cells) represent a potentially powerful approach to the treatment invasive brain tumors, such as malignant gliomas. We have previously shown that whether implanted directly into or at distant sites from an experimental intracranial glioma, NSCs distributed efficiently throughout main tumor mass also tracked advancing cells, while stably expressing reporter transgene. As therapeutic...

Brain metastases are an increasingly frequent and serious clinical problem for cancer patients, especially those with advanced melanoma. Given the extensive tropism of neural stem/progenitor cells (NSPCs) pathological areas in central nervous system, we expanded investigations to determine whether NSPCs could also target multiple sites brain a syngeneic experimental melanoma model. Using cytosine deaminase-expressing (CD-NSPCs) systemic 5-fluorocytosine (5-FC) pro-drug administration,...

Abstract A unique characteristic of neural stem cells is their capacity to track glioma that have migrated away from the main tumor mass into normal brain parenchyma. PEX, a naturally occurring fragment human metalloproteinase-2, acts as an inhibitor and endothelial cell proliferation, migration, angiogenesis. In present study, we evaluated antitumor activity PEX-producing against malignant glioma. The HB1.F3 line (immortalized cell) was transfected by pTracer vector with PEX. retention...

We report here a new mechanism for allelic discrimination--allele-specific Holliday Junction formation. The (HJ) is unique DNA structure that can be formed in sequence-nonspecific manner by routine PCR. To cause the PCR-based HJ formation to occur an allele-specific manner, PCR primers are manipulated such extra mismatch next SNP of interest introduced between target and reference amplicon GC-clamp added. Based on this mechanism, novel genotyping methods were developed, including homogeneous...

Classical late infantile neuronal ceroid lipofuscinosis (cLINCL) is a lysosomal storage disorder caused by mutations in CLN2 , which encodes tripeptidyl peptidase I (TPP1). Lack of TPP1 results accumulation autofluorescent material and curvilinear bodies cells throughout the CNS, leading to progressive neurodegeneration death typically childhood. In this study, we injected adeno-associated virus (AAV) vectors containing human cDNA into brains −/− mice determine therapeutic efficacy. AAV2 CU...

Development of the endocrine compartment pancreas, as represented by islets Langerhans, occurs through a series highly regulated events encompassing branching pancreatic epithelium, delamination and differentiation islet progenitors from ductal domains, followed expansion three-dimensional organization into clusters. Cellular interactions with extracellular matrix (ECM) mediated receptors integrin family are postulated to regulate key functions in these processes. Yet, specific developing...

Niemann-Pick disease is caused by a genetic deficiency in acid sphingomyelinase (ASM) leading to the intracellular accumulation of sphingomyelin and cholesterol lysosomes. In present study, we evaluated effects direct intracerebral transplantation neural progenitor cells (NPCs) on brain storage pathology ASM knock-out (ASMKO) mouse model Type A disease. NPCs derived from adult were genetically modified express human (hASM) transplanted into multiple regions ASMKO brain. Transplanted...

The spatial and temporal distribution of sucrose synthase (RSuS) in rice (Oryza sativa L.) was studied by Western immunohistochemical analyses using the mono-specific antibodies for three RSuS isoforms. In leaf tissues, RSuS1 localized mesophyll while RSuS2 phloem addition to mesophyll. roots, only found phloem. No RSuS3 could be detected any parts etiolated seedlings. expression each RSus gene is closely linked seed development. present aleurone layers developing seeds, at a low level...

As little as 5 micrograms of interstitial cell stimulating hormone (ICSH) or 20 ICSH-beta is effective for the induction ovulation in 100 percent hamsters treated at 0500 hours on day 4 after lordosis, whereas much 800 ICSH-alpha ineffective. Both ICSH and are also hypophysectomized animals. Thus, ovulation-inducing activity molecule resides its beta subunit.

Transforming growth factor β (TGFβ) is a key regulator associated with the pathogenesis of gingival overgrowth (GO). Connective tissue (CTGF/CCN2) overexpressed in GO tissues. CCN2 promotes and sustains fibrosis initiated by TGFβ. Previous studies have shown that JNK Smad3 activation required for TGFβ-induced expressions human fibroblasts (HGFs). In this study, we found Src major signaling mediator HGFs. Pre-treatment 2 kinase inhibitors (PP2, inhibitor-1) significantly reduced TGFβ1-induced...

There are no well-established indications for the surgical management of acute necrotizing pneumonitis in children. This study presents our experience regarding this challenging topic.Between 2002 and 2009, 56 patients with empyema were treated surgically. The outcomes analyzed retrospectively. Computed tomography findings massive lung necrosis or large cavities involving more than 50% involved lobe deemed to be complicated pneumonitis. Patients without above considered...

Abstract Objective To assess the association between clinical integration and financial integration, quality‐focused care delivery processes, beneficiary utilization outcomes. Data Sources Multiphysician practices in 2017‐2018 National Survey of Healthcare Organizations Systems (response rate 47%) 2017 Medicare claims data. Study Design Cross‐sectional study beneficiaries attributed to physician practices, focusing on two domains integration: (coordination patient services, use protocols,...

Abstract Sperm penetration through zona pellucida was observed in tubal fluid containing eggs and spermatozoa, which had been recovered under a layer of paraffin oil previously insufflated with mixture 5% CO 2 95% air warmed to 38°C before each experiment. A Nikon F 35 mm camera Bolex Rex‐5 16 movie camera, synchronized Xenon‐gas flash epuipment, were used microscope for recording this fast‐occurring phenomenon. In golden hamsters, 284, 306, 312 293 after 4, 4.5, 5 5.5 hours, respectively,...

Transforming growth factor β (TGFβ) regulates cell proliferation, differentiation, migration, apoptosis, and extracellular matrix production. It also plays a pivotal role in the pathogenesis of gingival overgrowth. Thrombin is key player tissue repair, remodeling, fibrosis after an injury, it exerts profibrotic effects by activating protease-activated receptors. Connective (CTGF or CCN2) modulates adhesion, production, wound healing. overexpressed many fibrotic disorders, including...

Progress in the functional studies of human olfactory receptors has been largely hampered by lack a reliable experimental model system. Although transgenic approaches mice could characterize function individual receptors, presence over 300 genes genome becomes daunting task. Thus, characterization individuals with genetic susceptibility to altered olfaction coupled absence particular receptor will allow phenotype/genotype correlations and vindicate specific their cognate ligands. We...

The development and function of epithelia depend on the establishment maintenance cell-cell adhesion intercellular junctions, which operate as mechanosensor hubs for transduction biochemical signals regulating cell proliferation, differentiation, survival, regeneration. Here, we show that αE-catenin, a key component adherens functions positive regulator pancreatic islet lineage differentiation by repressing sonic hedgehog pathway (SHH). Thus, deletion αE-catenin in multipotent progenitors...