A5100421264- Fungal and yeast genetics research
- Genetics, Aging, and Longevity in Model Organisms
- Gene Regulatory Network Analysis
- Fungal Biology and Applications
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Protein Tyrosine Phosphatases
- Genetic Neurodegenerative Diseases
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Single-cell and spatial transcriptomics
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
- Metabolism and Genetic Disorders
- Metabolomics and Mass Spectrometry Studies
- PI3K/AKT/mTOR signaling in cancer
- Privacy-Preserving Technologies in Data
- Signaling Pathways in Disease
- Stochastic Gradient Optimization Techniques
- Redox biology and oxidative stress
- Ferroptosis and cancer prognosis
- Phytochemical Studies and Bioactivities
- Plant-Microbe Interactions and Immunity
Lanzhou University
2024
Lanzhou University Second Hospital
2024
Runze (China)
2024
University of California, Berkeley
2021-2024
University of California, San Diego
2017-2023
Wenzhou Medical University
2022
First Affiliated Hospital of Wenzhou Medical University
2022
Huazhong University of Science and Technology
2020
Xiangya Hospital Central South University
2020
Central South University
2020
Autophagy may represent a common cellular response to nanomaterials, and modulation of autophagy holds great promise for improving the efficacy cancer therapy. Fullerene C60 possesses potent anti-cancer activities, but its considerable toxicity towards normal cells hinder practical applications. It has been reported that fullerene induces certain hallmarks in cells. Here we show water-dispersed nanocrystal underivatized (Nano-C60) at noncytotoxic concentrations caused authentic sensitized...
Programmed aging in yeast cells Following the fate of individual has revealed to be more a programmable decision process rather than simple accumulation deleterious events. Li et al. combined single-cell studies and mathematical modeling show that showed two different forms aging: one with less ribosomal DNA silencing, which nucleoli were degraded, another heme hemedependent transcription, mitochondria affected. Overexpression lysine deacetylase Sir2, contributes led third cell-aging average...
Abstract Tandem repeats (TRs) represent one of the largest sources genetic variation in humans and are implicated a range phenotypes. Here we present deep characterization TR based on high coverage whole genome sequencing from 3550 diverse individuals 1000 Genomes Project H3Africa cohorts. We develop method, EnsembleTR, to integrate genotypes four separate methods resulting high-quality at more than 1.7 million loci. Our catalog reveals novel sequence features influencing heterozygosity,...
Significance Aging is an inevitable consequence of living, and with it comes increased morbidity mortality. Novel approaches to mitigating age-related chronic diseases demand a better understanding the biology aging. Studies in model organisms have identified many conserved molecular factors that influence The emerging challenge understand how these interact change dynamically drive Using multidisciplinary technologies, we revealed sirtuin-dependent intermittent pattern chromatin silencing...
Abstract Plant root architecture dynamically adapts to various environmental conditions, such as salt‐containing soil. The phytohormone abscisic acid (ABA) is involved among others also in these developmental adaptations, but the underlying molecular mechanism remains elusive. Here, a novel branch of ABA signaling pathway Arabidopsis involving PYR/PYL/RCAR (abbreviated PYLs) receptor‐protein phosphatase 2A (PP2A) complex that acts parallel canonical PYLs‐protein 2C (PP2C) identified....
Chromatin instability and protein homeostasis (proteostasis) stress are two well-established hallmarks of aging, which have been considered largely independent each other. Using microfluidics single-cell imaging approaches, we observed that, during the replicative aging
Fritillaria is a well-known health-promoting food, but it has many varieties and its market circulation chaotic. In order to explore the differences in volatile organic compounds (VOCs) among different of quickly accurately determine variety Fritillaria, this study selected six identified analyzed their components using gas chromatography-ion mobility spectrometry (GC-IMS), establishing characteristic fingerprints VOCs Fritillaria. all samples, total 76 peaks were detected 67 identified. It...
The hematopoietic protein tyrosine phosphatase (HePTP) is implicated in the development of blood cancers through its ability to negatively regulate mitogen-activated kinases (MAPKs) ERK1/2 and p38. Small-molecule modulators HePTP activity may become valuable treating malignancies such as T cell acute lymphoblastic leukemia (T-ALL) myelogenous (AML). Moreover, compounds will further elucidate regulation MAPKs cells. Although transient activation crucial for growth proliferation, prolonged...
The pheromone response pathway of the yeast Saccharomyces cerevisiae is a well-established model for study G proteins and mitogen-activated protein kinase (MAPK) cascades. Our longstanding ability to combine sophisticated genetic approaches with established functional assays has provided thorough understanding signalling mechanisms regulation. In this report, we compare new methods used quantify pheromone-dependent MAPK phosphorylation, transcriptional induction, mating morphogenesis,...
Chronic obstructive pulmonary disease (COPD) is a serious chronic respiratory disorder. One of the major risk factors for COPD progression aging. Therefore, we investigated aging-related genes in using bioinformatic analyses. Firstly, Aging Atlas database containing 500 and Gene Expression Omnibus (GSE38974) were utilized to screen candidates. A total 24 candidate identified related both Using gene ontology Kyoto Encyclopedia Genes Genomes enrichment analyses, found that this list was...
Ras proteins and cAMP-dependent protein kinase (protein A, PKA) are important components of a nutrient signaling pathway that mediates cellular responses to glucose in yeast. The molecular mechanisms regulate Ras/PKA-mediated remain be fully understood. Here, we provide evidence Ras/PKA is negatively regulated by deubiquitinating enzyme, Ubp3. Disrupting the activity Ubp3 leads hyperactivation PKA, as evidenced much enhanced phosphorylation PKA substrates, decreased accumulation glycogen,...
In the yeast Saccharomyces cerevisiae, exposure to mating pheromone activates a prototypic mitogen-activated protein kinase (MAPK) cascade and triggers dose-dependent differentiation response. Whereas high dose induces growth arrest formation of shmoo-like morphology in cells, lower doses elicit elongated cell growth. Previous population-level analysis has revealed that MAPK Fus3 plays an important role mediating this switch. To further investigate how controls fate decision process at...
Aging is a complex, yet pervasive phenomenon in biology. As human cells steadily succumb to the deteriorating effects of aging, so too comes host age-related ailments such as neurodegenerative disorders, cardiovascular disease and cancer. Therefore, elucidation molecular networks that drive aging paramount importance health. Progress toward this goal has been aided by studies from simple model organisms Saccharomyces cerevisiae. While work budding yeast already revealed much about basic...
Abstract Short tandem repeats (STRs), genomic regions each consisting of a sequence 1-6 base pairs repeated in succession, represent one the largest sources human genetic variation. However, many STR effects are not captured well by standard genome-wide association studies (GWAS) or downstream analyses that mostly based on single nucleotide polymorphisms (SNPs). To study involvement STRs complex traits, we imputed genotypes for 445,720 autosomal into genotype array data from 408,153 White...
Sumoylation, a post-translational protein modification by small ubiquitin-like modifier (SUMO), has been implicated in many stress responses. Here we analyzed the potential role of sumoylation osmo-response yeast. We find that osmotic induces rapid accumulation sumoylated species normal yeast cells. Interestingly, disruption MAP kinase Hog1 leads to much higher level conjugates are independent new synthesis. also is dependent on SUMO ligase Siz1. Notably, overexpression SIZ1 HOG1-disruption...
Abstract Objectives Bavachin is a bioactive natural flavonoid with oestrogen-like activity. Here, we aimed to investigate its metabolic and disposal fates involving in CYPs, UGTs efflux transporters. Methods Phase I metabolism glucuronidation were performed by human liver microsomes (HLM). Reaction phenotyping activity correlation analysis identify the main CYP UGT isozymes. Chemical inhibition gene knock-down approaches employed explore function of BCRP MRPs. Key findings Five phase...
The development of the neuromuscular junction depends on signaling processes that involve protein phosphorylation. Motor neuron releases agrin to activate muscle Dok-7, a key tyrosine kinase essential for formation mature and functional junction. However, cascade downstream Dok-7 remains poorly understood. In this study, we combined clustered regularly interspaced short palindromic repeats/Cas9 technique quantitative phosphoproteomics analysis study phosphorylation events triggered by...
Abstract: Bladder cancer (BC) is a common malignant tumor of the urinary system. While current approaches involving adjuvant chemotherapy, radiotherapy, and immunotherapy have shown significant progress in BC treatment, challenges, such as recurrence drug resistance, persist, especially case muscle-invasive bladder (MIBC). It mainly due to lack pre-existing immune response cells microenvironment. Micro-environmental changes (such hypoxia under-nutrition) can cause aggregation unfolded...
ABSTRACT Filamentous fungi are important producers of enzymes and secondary metabolites. The industrial thermophilic species, Thermothelomyces thermophilus, is closely related to the model fungus, Neurospora crassa . A critical aspect filamentous fungal life cycle production asexual spores (conidia), which regulated by various stimuli, including nutrient availability. Several species fungi, T. thermophilus , produce conidia under submerged fermentation conditions, can be detrimental product...
A passive (power-less), bending type MEMS DC current sensor to satisfy the increasing needs of power supply for monitoring electricity consumption by either one-wire or two-wire appliance cord was proposed in our past work (DTIP 2011). MEMS-scale prototype sensor, comprised 5 parallel PZT plates, then micro-fabricated preliminarily examination 2012). novel oscillating both actuating and sensing elements, further electric appliances (DTIP2013). Then ANSYS analytical model cantilever-based...
As the application of federated learning becomes increasingly widespread, issue imbalanced training data distribution has emerged as a significant challenge. Federated utilizes local stored on different clients for model training, rather than centralizing server, thereby greatly enhancing privacy and security data. However, across may be imbalanced, with categories potentially residing clients. This presents challenge to traditional learning, which assumes is independent identically...