A5088010191- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Receptor Mechanisms and Signaling
- Chemokine receptors and signaling
- Complement system in diseases
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Eosinophilic Esophagitis
- Immune cells in cancer
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Geophysics and Gravity Measurements
- Occupational exposure and asthma
- Biochemical Analysis and Sensing Techniques
- Pediatric health and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Oceanographic and Atmospheric Processes
- Ocean Waves and Remote Sensing
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
University of Lübeck
2014-2023
German Center for Lung Research
2014
Abstract C3a exerts multiple biologic functions through activation of its cognate receptor. C3−/− and C3aR−/− mice have been instrumental in defining important roles the C3a/C3aR axis regulation acute chronic inflammatory diseases, including ischemia/reperfusion injury, allergic asthma, autoimmune nephritis, rheumatoid arthritis. Surprisingly little is known about C3aR expression function immune stromal cells. To close this gap, we generated a floxed tandem-dye Tomato (tdTomato)–C3aR...
Abstract The biological significance of C5a receptor [(C5aR)2/C5L2], a seven-transmembrane binding and C5adesArg, remains ill-defined. Specific ligation C5aR2 inhibits C5a-induced ERK1/2 activation, strengthening the view that regulates C5aR1-mediated effector functions. Although C5aR1 are often coexpressed, detailed picture expression in murine cells tissues is still lacking. To close this gap, we generated floxed tandem dye (td)Tomato–C5aR2 knock-in mouse used to track tissue-residing...
Pulmonary eosinophils comprise at least two distinct populations of resident (rEOS) and inflammatory (iEOS), the latter recruited in response to pulmonary inflammation. Here, we determined impact complement activation on rEOS iEOS trafficking function models inflammation.BALB/c wild-type C5ar1-/- mice were exposed different allergens or IL-33. Eosinophil airways, lung, mediastinal lymph nodes (mLN) characterized by FACS immunohistochemistry. functions vivo vitro.HDM IL-33 exposure induced a...
Conventional dendritic cells (cDC) are necessary and sufficient to drive mixed maladaptive Th2/Th17 immune responses toward aeroallergens in experimental allergy models. Previous studies suggest that the anaphylatoxin C3a promotes, whereas C5a protects from development of immunity during allergen sensitization. However, only limited evidence exists such effects directly mediated through anaphylatoxin-receptor signaling cDCs. In this study, we assessed impact on cDC-mediated induction...
C5a drives airway constriction and inflammation during the effector phase of allergic asthma, mainly through activation receptor 1 (C5aR1). Yet, C5aR1 expression on myeloid lymphoid cells is ill-defined. Recently, we generated characterized a floxed green fluorescent protein (GFP)-C5aR1 knock-in mouse. Here, used this reporter strain to monitor in airway, pulmonary lymph node OVA-driven asthma. wildtype mice developed similar phenotype with comparable resistance, mucus production,...
C5a regulates the development of maladaptive immune responses in allergic asthma mainly through activation receptor 1 (C5aR1). Yet, cell types and mechanisms underlying this regulation are ill-defined. Recently, we described increased C5aR1 expression lung tissue eosinophils but decreased airway pulmonary macrophages as well CD11b+ conventional dendritic cells (cDCs) monocyte-derived DCs (moDCs) during effector phase using a floxed green fluorescent protein (GFP)-C5aR1 knock-in mouse. Here,...
Abstract Background A close association between obesity and asthma has been described. The nature of this remains elusive, especially with respect to allergic asthma. Controversial findings exist regarding the impact short‐term high‐fat diet (HFD) feeding on development Objective To delineate HFD experimental Methods Female C57BL/6JRJ mice were fed a or chow (CD) for 12 weeks. Allergic was induced by intraperitoneal OVA/alum sensitization followed repeated OVA airway challenges. We...
Abstract The anaphylatoxin C3a exerts its biologic functions through the activation of cognate G-protein-coupled receptor (C3aR). C3aR mRNA expression has been described both in human and mouse lungs. However, no systematic analysis at steady state under pro-inflammatory conditions different pulmonary cell subsets done so far. Using a newly developed Tandem-dye (td)-Tomato-C3aR reporter knock-in mouse, we found eosinophils, resident CD11b+ conventional dendritic cells (cDC), monocyte-derived...
Abstract Eosinophils are considered a homogenous cell population, contributing to the allergic asthma phenotype by promoting mucus hypersecretion and airway hyperresponsiveness. Here, we identified previously unrecognized vacuolated CD11cdim eosinophilic population (vEOS) which accmulates exclusively in lung tissue mediastinal lymph nodes but not airways house dust mite-mediated model an IL-33 mediated pulmonary inflammation. In addition vEOS, found classical SiglecF+CD11c− eosinophils (EOS)...