A5053360949- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- CRISPR and Genetic Engineering
- Peatlands and Wetlands Ecology
- Soil Carbon and Nitrogen Dynamics
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Insect symbiosis and bacterial influences
- Biochemical and Structural Characterization
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Malaria Research and Control
- Chromosomal and Genetic Variations
- Soil and Water Nutrient Dynamics
- Phytochemistry and Bioactive Compounds
- Cocoa and Sweet Potato Agronomy
- Advanced ceramic materials synthesis
- Travel-related health issues
- Bioenergy crop production and management
- Allelopathy and phytotoxic interactions
- Plant Surface Properties and Treatments
- Plant responses to elevated CO2
- Metal-Catalyzed Oxygenation Mechanisms
- Light effects on plants
- Plant Virus Research Studies
Fraunhofer Institute for Molecular Biology and Applied Ecology
2013-2025
John Wiley & Sons (United States)
2015
Fraunhofer Society
2004-2006
RWTH Aachen University
2005
Swedish University of Agricultural Sciences
1996-2001
Umeå Plant Science Centre
1998-2001
Max Planck Institute for Biogeochemistry
2000
Abstract Several isoforms of superoxide dismutase (SOD) with a high isoelectric point (pI) have been identified by focusing chromatography in protein extracts from Scots pine (Pinus sylvestris) needles. One these isoforms, CuZn-SOD pI about 10 and thus denoted hipI-SOD, has isolated purified to apparent homogeneity. A cDNA encoding the hipI-SOD was cloned sequenced. Northern hybridization mRNA different organs tissues showed that markedly pattern expression compared chloroplastic cytosolic...
Plant cell suspension cultures can be used for the production of recombinant pharmaceutical proteins, but their potential is limited by modest levels that may unstable over long culture periods, reflecting initial heterogeneity and subsequent genetic epigenetic changes. We flow sorting to generate highly productive monoclonal lines from a heterogeneous population tobacco BY-2 cells expressing human antibody M12 selecting co-expressed fluorescent marker protein DsRed located on same T-DNA....
The high fibrin specificity of Desmodus rotundus salivary plasminogen activator alpha1 (DSPAalpha1 or desmoteplase (INN)) makes it a promising candidate for the treatment acute ischemic stroke. In current study we explored use transgenic tobacco plants and BY-2 suspension cells as alternative production platforms this drug. Four different N-terminal signal peptides, from animals, were used to translocate recombinant DSPAalpha1 protein endomembrane system. Intact was produced in cells,...
The economically efficient, reproducible cultivation of plants containing valuable ingredients for pharmaceutical or cosmetic purposes is a challenge today. Although greenhouse much more expensive than field cultivation, this may be justified by the high level control over environmental conditions. However, careful analysis costs and investigation potential cost-reducing measures are essential. Here, soapwort (Saponaria officinalis) was grown in to identify factors influencing production...
Pf38 is a surface protein of the malarial parasite Plasmodium falciparum. In this study, we produced and purified recombinant fusion composed red fluorescent (RFP-Pf38) using transient expression system in plant Nicotiana benthamiana. To our knowledge, first description production Pf38. verify quality Pf38, plasma from semi-immune African donors was used to confirm specific binding ELISA measurements revealed that immune responses subset were comparable reactivities AMA-1 MSP119. RFP-Pf38...
Genome modification by homology-directed repair (HDR) is an attractive tool for the controlled genetic manipulation of plants. Here, we report HDR-mediated gene exchange expression cassettes in tobacco BY-2 cells using a designed zinc finger nuclease (ZFN). The target contained 7-kb fragment flanked two ZFN cutting sites. That was replaced with 4-kb donor cassette, which integrates markers selection (kanamycin resistance) and scoring targeting (red fluorescent protein, RFP). Candidates...
Summary Transgenic hybrid aspen ( Populus tremula L. × P. tremuloides Michx.) plants expressing a high‐isoelectric‐point superoxide dismutase (hipI‐SOD) gene in antisense orientation were generated to investigate its function. Immunolocalization studies showed the enzyme be localized extracellularly, secondary cell wall of xylem vessels and phloem fibers. The lines hipI‐SOD exhibited distinct phenotype; growth rate was reduced, stems thinner leaves smaller than wild‐type (WT) plants....
Abstract The functional analysis of plant cells at the cellular and subcellular levels requires novel technologies for directed manipulation individual cells. In this report, we demonstrate use an infrared (1,064 nm) picosecond laser perforation tobacco cell protoplasts. A single pulse was sufficient to perforate plasma membrane enabling uptake dye from surrounding medium into cytosol. Moreover, procedure shown be suitable efficient delivery DNA expression constructs nucleus, as demonstrated...
We measured fine root N concentration, in vivo nitrate reductase activity (NRA) and uptake capacity for (15)NH(4) (+) (15)NO(3) (-) along an N-deposition gradient from northern Sweden to central Europe, encompassing a variation deposition rates of < 5 about 40 kg ha(-1) year(-1). The focus was on Picea abies (L.) Karst., but Fagus sylvatica L. Europe Pinus sylvestris Betula spp. were also studied. assumed that, with increased supply N, concentration would increase, the inducible enzyme (NR)...
Extracellular superoxide dismutase was found in extraxylematic bark tissues and seedlings of Pinus sylvestris L. The isozymes were partially purified by infiltration analysed polyacrylamide gel electrophoresis. One the major isoforms to homogeneity N-terminal amino acid sequence determined. isoelectric point these extracellular forms is distinctly higher than that cellular dismutases. Furthermore, a novel type with an nine detected crude needle extract.
Targeted integration of recombinant DNA fragments into plant genomes by double-strand break (DSB) repair mechanisms has become a powerful tool for precision engineering crops. However, many targeting platforms require the screening transgenic events to identify low number targeted among more random insertion events. We developed an engineered transgene platform (ETIP) that uses incomplete marker genes at site enable rapid phenotypic and recovery upon functional reconstitution genes. The two...
We demonstrated the successful optimization of a recombinant multi-subunit malaria vaccine candidate protein for production in methylotrophic yeast Pichia pastoris by identification and subsequent removal two protease cleavage sites. After observing degradation culture supernatant fed-batch fermentation, predominant proteolytic fragment secreted was analyzed mass spectrometry. The MS data indicated an amino acid sequence matching KEX2-protease consensus motif EKRE. this region completely...
Abstract The development of low‐alkaloid (LA) tobacco varieties is an important target in the breeding industry. However, LA Burley 21 plants, which Nic1 and Nic2 loci controlling nicotine biosynthesis are deleted, characterized by impaired leaf maturation that leads to poor quality before after curing. Polyamines involved key developmental, physiological, metabolic processes act as anti‐senescence anti‐ripening regulators. We investigated role polyamines analyzing free conjugated polyamine...
Abstract Genome editing tools such as zinc-finger nucleases provide novel strategies for genetic manipulation in plants. Unlike agrobacterium-mediated or direct gene transfer, which introduce genes randomly into the genome and thereby potentially resulting high variation of expression, targeted addition provides predictable integration DNA sequences a specified location plant genome. We investigated whether various independent cell lines that all contain transgene placed same genomic locus...
Traditional breeding and molecular approaches have been used to develop tobacco varieties with reduced nicotine secondary alkaloid levels. However, available low-alkaloid impaired leaf quality likely due the metabolic consequences of biosynthesis downregulation. Recently, we found evidence that unbalanced crosstalk between polyamine pathways is involved in ripening a (LA) Burley 21 line having mutation at Nic1 Nic2 loci, key biosynthetic regulators biosynthesis. Since loci are comprised...
Malaria still represents a major cause of morbidity and mortality predominantly in several developing countries, remains priority many public health programmes. Despite the enormous gains made control prevention development an effective vaccine persisting challenge. Although parasite antigens including pre-erythrocytic blood stage have been thoroughly investigated, identification solid immune correlates protection against infection by Plasmodium falciparum or clinical malaria hurdle. In this...
Abstract In this work, thermally sprayed sustainable coatings with spray additives recycled from dry alkaline batteries and solid-oxide fuel cells are developed to allow the growth of drought-resistant plants like moss, microclover chamomile. It is assumed that these anchor coating their rhizoids hence can be grown without presence soil. Preliminary tests a Yttrium Stabilized Zirconia (YSZ) ceramic on sheet metal confirms chamomile plant.