Sigrid Noreng
A5012968177- Methane Hydrates and Related Phenomena
- Seismic Waves and Analysis
- Seismology and Earthquake Studies
- Earthquake Detection and Analysis
- Geophysical Methods and Applications
- Ion Transport and Channel Regulation
- Advanced Electron Microscopy Techniques and Applications
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Chemokine receptors and signaling
- Machine Learning in Materials Science
- Silicon Carbide Semiconductor Technologies
- Cardiac Arrhythmias and Treatments
- Enzyme Structure and Function
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Chemical Physics Studies
- Photoacoustic and Ultrasonic Imaging
- Electrolyte and hormonal disorders
- Magnetic and Electromagnetic Effects
- Photosynthetic Processes and Mechanisms
- Advancements in Semiconductor Devices and Circuit Design
- Ion Channels and Receptors
- RNA and protein synthesis mechanisms
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
Oregon Health & Science University
2018-2022
Vollum Institute
2020
University of Oregon
2013
The epithelial sodium channel (ENaC), a member of the ENaC/DEG superfamily, regulates Na+ and water homeostasis. ENaCs assemble as heterotrimeric channels that harbor protease-sensitive domains critical for gating channel. Here, we present structure human ENaC in uncleaved state determined by single-particle cryo-electron microscopy. ion is composed large extracellular domain narrow transmembrane domain. reveals assembles with 1:1:1 stoichiometry α:β:γ subunits arranged counter-clockwise...
NOX2 is the prototypical member of NADPH oxidase NOX superfamily and produces superoxide (O2•-), a key reactive oxygen species (ROS) that essential in innate adaptive immunity. Mutations lead to deficiency activity correlate with increased susceptibility bacterial fungal infections, resulting chronic granulomatous disease. The core formed by heterodimeric transmembrane complex composed (formerly gp91) p22, but detailed description its structural architecture lacking. Here, we present...
The molecular bases of heteromeric assembly and link between Na + self-inhibition protease-sensitivity in epithelial sodium channels (ENaCs) are not fully understood. Previously, we demonstrated that ENaC subunits – α, β, γ assemble a counterclockwise configuration when viewed from outside the cell with protease-sensitive GRIP domains periphery (Noreng et al., 2018). Here describe structure resolved by cryo-electron microscopy at 3 Å. We find combination precise domain arrangement...
The C-C motif chemokine receptor 8 (CCR8) is a class A G-protein coupled that has emerged as promising therapeutic target in cancer. Targeting CCR8 with an antibody appeared to be attractive approach, but the molecular basis for chemokine-mediated activation and antibody-mediated inhibition of are not fully elucidated. Here, we obtain antagonist against human determine structures complex either or endogenous agonist ligand CCL1. Our studies reveal characteristic features allowing recognition...
The mechanical rigidity of lipid membranes is a key determinant the energetics cellular membrane deformation. Measurements bending moduli remain rare, however, and show large variance, situation that can be addressed by development improved techniques comparisons between disparate applied to same systems. We introduce here use selective plane illumination microscopy (SPIM, also known as light sheet fluorescence microscopy) image thermal fluctuations giant vesicles. optical sectioning SPIM...
The EMDataResource Ligand Model Challenge aimed to assess the reliability and reproducibility of modeling ligands bound protein protein/nucleic-acid complexes in cryogenic electron microscopy (cryo-EM) maps determined at near-atomic (1.9-2.5 Å) resolution. Three published were selected as targets: E. coli beta-galactosidase with inhibitor, SARS-CoV-2 RNA-dependent RNA polymerase covalently nucleotide analog, ion channel ORF3a lipid. Sixty-one models submitted from 17 independent research...