A5005462593- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Neurogenesis and neuroplasticity mechanisms
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Pluripotent Stem Cells Research
- Lipid Membrane Structure and Behavior
- Axon Guidance and Neuronal Signaling
- Anesthesia and Neurotoxicity Research
- Immune Response and Inflammation
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Adipose Tissue and Metabolism
- Pharmacological Effects of Natural Compounds
Innsbruck Medical University
2015-2023
Universität Innsbruck
2021
Czech Academy of Sciences, Institute of Physiology
2015
Significance Voltage-gated calcium channels are important regulators of neuronal functions, as for example synaptic transmission. Their auxiliary α 2 δ subunits modulating the currents. Beyond that they have emerged modulators functions. Here, we established a cellular triple knockout/knockdown model in cultured hippocampal neurons by knocking out or down expression all three expressed brain. Our experiments demonstrate presynaptic loss proteins leads to severe defect glutamatergic synapse...
Presynaptic α 2 δ subunits of voltage-gated calcium channels regulate channel abundance and are involved in glutamatergic synapse formation. However, little is known about the specific functions individual isoforms their role GABAergic synapses. Using primary neuronal cultures embryonic mice both sexes, we here report that presynaptic overexpression δ-2 synapses strongly increases clustering postsynaptic GABA A Rs. Strikingly, exerts same effect synapses, leading to a mismatched localization...
Nociceptors are primary afferent neurons serving the reception of acute pain but also transit into maladaptive disorders. Since native human nociceptors hardly available for mechanistic functional research, and rodent models do not necessarily mirror pathologies in all aspects, induced pluripotent stem cell-derived (iDN) offer superior advantages as a model system. Unbiased mRNA::microRNA co-sequencing, immunofluorescence staining, qPCR validations, reveal expression trajectories well miRNA...
Kalpachidou, Theodora; Riehl, Lydia; Schöpf, Clemens L.; Ucar, Buket; Kress, Michaela Author Information
Auxiliary α 2 δ subunits of voltage-gated calcium channels modulate channel trafficking, current properties, and synapse formation. Three the four isoforms (α δ-1, δ-2, δ-3) are abundantly expressed in brain; however, available knockout models, only δ-2 or mutant mice display an obvious abnormal neurological phenotype. Thus, we hypothesize that neuronal may have partially specific as well redundant functions. To address this, generated three distinct double mouse models by crossbreeding...
In nerve cells the genes encoding for α 2 δ subunits of voltage-gated calcium channels (VGCCs) have been linked to synaptic functions and neurological disease. Here we show that are essential formation organization glutamatergic synapses. Using a cellular subunit triple loss-of-function model, demonstrate failure in presynaptic differentiation associated with downscaling postsynaptic AMPA receptors density. The role isoforms as organizers is highly redundant, each individual isoform can...
Abstract The prototypical pleiotropic anti-inflammatory cytokine IL-4 not only acts on immune cells but also has important roles in the nervous system mediating antinociception and neuroregeneration. Therefore, we explored expression of IL-4, its receptors IL4RA IL13RA1 downstream signaling components together with morphological functional assays as well transcriptomic profiling human nociceptors differentiating from induced pluripotent stem (iNocs). de novo formation synaptic boutons...
Abstract Nociceptors are primary afferent neurons serving the reception of acute pain but also transit into maladaptive disorders. Since native human nociceptors hardly available for mechanistic functional research, and rodent models do not necessarily mirror pathologies in all aspects, iPSC-derived (iDN) offer superior advantages as a model system. Unbiased mRNA::microRNA co-sequencing, immunofluorescence staining qPCR validations, revealed expression trajectories well miRNA target spaces...