In situ tissue engineering is a technology in which non-cellular biomaterial scaffolds are implanted order to induce local regeneration of replaced or damaged tissues. Degradable synthetic electrospun versatile and promising class biomaterials for various applications, such as cardiovascular replacements. Functional depends on the balance between endogenous neo-tissue formation scaffold degradation. Both these processes driven by macrophages. Upon invasion into scaffold, macrophages secrete...

Bioengineered grafts have the potential to overcome limitations of autologous and non-resorbable synthetic vessels as vascular substitutes. However, one challenges in creating these living is induce maintain multiple cell phenotypes with a biomimetic organization. Our heterotypic design hold promises for functional neovessel regeneration by guiding layered cellular tissue organization into native-like structure. In this study, perfusable two-compartment bioreactor chamber was designed...

The success of cardiovascular tissue engineering (TE) strategies largely depends on the mechanical environment in which cells develop a neotissue through growth and remodeling processes. This is defined by local scaffold architecture to adhere, that is, microenvironment, external cues respond, hemodynamic loading. early developing blood vessels consists both shear stress (due flow) circumferential stretch pressure). Experimental platforms recapitulate this controlled tunable manner are thus...

Biomaterials are increasingly used for in situ vascular tissue engineering, wherein resorbable fibrous scaffolds implanted as temporary carriers to locally initiate regeneration. Upon implantation, macrophages infiltrate and start degrading the scaffold, while simultaneously driving a healing cascade via secretion of paracrine factors that direct behavior tissue-producing cells. This balance between neotissue formation scaffold degradation must be maintained at all times ensure graft...

Abstract Resorbable synthetic scaffolds designed to regenerate living tissues and organs inside the body have emerged as a clinically attractive technology replace diseased blood vessels. However, mismatches between scaffold design in vivo hemodynamic loading (i.e., cyclic stretch shear stress) can result aberrant inflammation adverse tissue remodeling, leading premature graft failure. Yet, underlying mechanisms remain elusive. Here, human vitro model is presented that mimics transient local...

Pressure ulcers are a type of local soft tissue injury due to sustained mechanical loading and remain common issue in patient care. People with spinal cord (SCI) especially at risk pressure impaired mobility sensory perception. The development load improving support structures relies on realistic evaluation e.g. using finite element analysis (FEA). FEA requires subject-specific properties geometries. This study focuses the effect geometry. MRI is used for creation geometrically accurate...

There is a continuous search for the ideal bioresorbable material to develop scaffolds in situ vascular tissue engineering. As these are exposed harsh hemodynamic environment during entire transformation process from scaffold neotissue, it of crucial importance maintain mechanical integrity and stability at all times. Bilayered made supramolecular polycarbonate-ester-bisurea were manufactured using dual electrospinning. These contained porous inner layer allow cellular infiltration dense...

The regenerative potential of articular cartilage (AC) defects is limited and depends on defect size, biomechanical conditions, age. Early events after overloading might be predictive for degeneration in the long term. Therefore, present aim to investigate temporal response at cell, matrix, tissue level during first period mechanical overloading. In study, effect high loading (∼8 MPa) a rate (∼14 MPa/s) day 0 9 collagen damage, gene expression, cell death, biochemical composition AC was...

The mechanical properties of scaffolds used for mechanically challenging applications such as cardiovascular implants are unequivocally important. Here, the effect supramolecular additive functionalization on behavior electrospun was investigated one bisurea-based model and two previously developed antifouling additives. has no bulk material, whereas stiffness slightly decreased compared to pristine PCL-BU following addition three different These results show robustness additives in...

Uniaxial ring test is a widely used mechanical characterization method for variety of materials, from industrial elastomers to biological materials. Here we show that the combination local material compression, bending, and stretching during uniaxial results in geometry-dependent deformation profile can introduce systematic errors extraction parameters. We identify stress strain regimes under which dominates develop simple image-based analysis approach eliminates these errors. rigorously...

Abstract Disturbed shear stress is thought to be the driving factor of neointimal hyperplasia in blood vessels and grafts, for example hemodialysis conduits. Despite common occurrence hyperplasia, however, mechanistic role unclear. This especially problematic context situ scaffold-guided vascular regeneration, a process strongly driven by scaffold mechanical environment. To address this issue, we herein introduce an integrated numerical-experimental approach reconstruct graft–host response...

The use of resorbable biomaterials to induce regeneration directly in the body is an attractive strategy from a translational perspective. Such materials inflammatory response upon implantation, which driver subsequent resorption material and new tissue. This strategy, also known as situ tissue engineering, pursued obtain cardiovascular replacements such tissue-engineered vascular grafts. Both regenerative processes are determined by local biomechanical cues on scaffold (i.e., stretch shear...

The use of resorbable biomaterials to induce regeneration directly in the body is an attractive strategy from a translational perspective. Such materials inflammatory response upon implantation, which driver subsequent resorption material and new tissue. This strategy, also known as situ tissue engineering, pursued obtain cardiovascular replacements such tissue-engineered vascular grafts. Both regenerative processes are determined by local biomechanical cues on scaffold (i.e., stretch shear...

Pressure ulcers are a type of local soft tissue injury due to sustainedmechanical loading and remain common issue in patient care. People withspinal cord (SCI) especially at risk pressure toimpaired mobility sensory perception. The development load improvingsupport structures relies on realistic evaluation e.g. usingfinite element analysis (FEA). FEA requires subject-specificmechanical properties geometries. This study focuses the effect ofgeometry. MRI is used for creation geometrically...

A bstract Disturbed shear stress is thought to be the driving factor of neointimal hyperplasia in blood vessels and grafts, for example hemodialysis conduits. Despite common occurrence hyperplasia, however, mechanistic role unclear. This especially problematic context situ scaffold-guided vascular regeneration, a process strongly driven by scaffold mechanical environment. To address this issue, we herein introduce an integrated numerical-experimental approach reconstruct graft-host response...

Introduction: In-situ engineering of vascular tissues, starting from a degradable synthetic scaffold, requires an optimal balance between scaffold degradation and neo-tissue formation to maintain mechanical function. Due their elastomeric properties, bis-urea (BU)-modified supramolecular polymers are attractive biomaterials for tissue engineering1,2. The susceptibility these enzymatic can be easily tuned by varying the amount ester groups in polymer backbone. However, it is unknown if how...

1 Abstract Resorbable synthetic scaffolds designed to regenerate living tissues and organs inside the body emerge as a clinically attractive technology replace diseased blood vessels. However, mismatches between scaffold design in vivo hemodynamic loading (i.e., cyclic stretch shear stress) can result aberrant inflammation adverse tissue remodeling, leading premature graft failure. Yet, underlying mechanisms remain elusive. Here, human vitro model is presented that mimics transient local...