A5010403234- Cervical Cancer and HPV Research
- Women's cancer prevention and management
- Hepatitis B Virus Studies
- T-cell and Retrovirus Studies
- Viral-associated cancers and disorders
- interferon and immune responses
- HIV Research and Treatment
- Science and Education Research
- Cancer-related molecular mechanisms research
- Immunodeficiency and Autoimmune Disorders
- Colorectal and Anal Carcinomas
- Herpesvirus Infections and Treatments
- Molecular Biology Techniques and Applications
- Urinary Tract Infections Management
- Cancer-related Molecular Pathways
- Plant Disease Resistance and Genetics
- HIV/AIDS drug development and treatment
- Virus-based gene therapy research
- Genital Health and Disease
- Reproductive tract infections research
- Immune Cell Function and Interaction
Harvard University
2019-2022
Instituto Nacional do Câncer
2012-2019
Oncovir (United States)
2013-2017
University of Minnesota
2014
Universidade Federal do Rio Grande
2011
Several recent studies have converged upon the innate immune DNA cytosine deaminase APOBEC3B (A3B) as a significant source of genomic uracil lesions and mutagenesis in multiple human cancers, including those breast, head/neck, cervix, bladder, lung, ovary, other tissues. A3B is upregulated these tumor types relative to normal tissues, but mechanism unclear. Because also has antiviral activity systems member broader response, we tested hypothesis that papillomavirus (HPV) infection causes...
OBJETIVO: Determinar a prevalência e os genótipos do HPV identificar fatores associados à infecção em mulheres, gestantes não HIV-1 positivas negativas, atendidas nos Ambulatórios de Ginecologia Obstetrícia Unidades Básicas Saúde Rio Grande, Grande Sul, Brasil.MÉTODOS: Amostras células cervicais 302 mulheres foram analisadas para presença por reação cadeia da polimerase, aninhada sequenciamento.Foram calculadas as razões associadas às variáveis estudadas meio teste exato Fisher ou χ 2...
Polymorphisms in genes that encode chemokines or their receptors can modulate susceptibility to human immunodeficiency virus (HIV) infection and disease progression. The objective of this study was assess the frequency polymorphisms CCR5-Δ32, CCR2-64I, CCR5-59029A SDF1-3'A role course HIV a Southern Brazilian population. Clinical data were obtained from 249 patients for an average period 6.4 years genotypes determined by standard polymerase chain reaction (PCR) PCR-restriction fragment...
OBJETIVO: Foi avaliar a prevalência de Chlamydia trachomatis e os fatores risco associados à infecção em amostras endocervicais mulheres atendidas ambulatório Ginecologia Obstetrícia. MÉTODOS: Amostras secreção endocervical 200 Hospital Universitário foram avaliadas para diagnosticar C. com uso da reação cadeia polimerase (PCR) utilizando primers CT05/CT06 que amplificam 281 pares bases principal proteína membrana externa trachomatis. Todas as participantes responderam um questionário...
Increasing evidence suggests that human papillomavirus (HPV) intratype variants (specific lineages and sublineages) are associated with pathogenesis progression from HPV infection to persistence the development of cervical cancer.This study aimed verify prevalence distribution types HPV16 in southern Brazil women normal cytology or intraepithelial lesions.HPV typing was determined by L1 gene sequencing. To identify variants, LCR E6 regions were sequenced, characteristic single nucleotide...
The classification of human papillomavirus (HPV) intratypic lineages by complete genome sequencing is a determinant in understanding biological differences association with this disease. In work, we have characterised HPV genomes from southern Brazil. Fifteen cervicovaginal Pap smear negative samples previously categorised as HPV-positive were sequenced using ultradeep sequencing, and 18 13 different types assembled. Phylogenetic genetic distance analyses performed to classify the into...
The APOBEC3 (A3) proteins are cytidine deaminases that exhibit the ability to insert mutations in DNA and/or RNA sequences. APOBEC3B (A3B) has been evidenced as a mutagen with consistent high expression several cancer types. Data concerning A3B influence on HPV infection and cervical limited controversial. We investigated role of levels affected women positive for by different Tumor biopsies from cancerous uterine cervix were collected 216 registered at Hospital do Câncer II Instituto...
The human papillomavirus (HPV) E5 gene encodes a small and highly hydrophobic oncoprotein that affects immune evasion, cell proliferation, loss of apoptotic capacity angiogenesis in tumors. shows an affinity for biological membranes was associated with increase epidermal growth factor/epidermal factor receptor (EGF/EGFR) signaling through the accumulation EGFR cellular membranes. Due to frequent integration HPV genome into host genome, is frequently not transcribed cervical tumors.In this...
Study design: cross-sectional. Objective: To determine the HPV prevalence and genotypes in women treated at University Hospital southern Brazil. Methodology: Cervical cells samples from 200 were collected. was detected by nested polymerase chain reaction determined sequencing. Variables analyzed Fisher Exact Test Chi-squared test of Pearson (X²) with a significance level ≤ 5%. The strength association calculated ratio, their confidence intervals 95%. Multivariate analysis Binary Logistic...
A entrada do Virus da Imunodeficiencia Humana na celula alvo requer interacao gp120 viral com o receptor CD4 e correceptores especificos. Mutacoes nos CCR5 CCR2, denominadas ccr5 32 ccr2-64I, sao associadas a diferencas progressao para AIDS. O objetivo deste estudo verificar frequencia destes alelos sua correlacao Amostras de sangue foram utilizadas contagem linfocitos TCD4, determinacao carga extracao DNA genomico. genotipagem foi realizada por PCR RFLP. alelo mutante esteve presente em...