A5075227995- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Anaerobic Digestion and Biogas Production
- Ruminant Nutrition and Digestive Physiology
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolites in Food Biotechnology
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Soil Carbon and Nitrogen Dynamics
- Membrane Separation Technologies
- Plant and fungal interactions
- Fungal Biology and Applications
- Microbial Community Ecology and Physiology
- Medicinal plant effects and applications
- Saffron Plant Research Studies
- Phytase and its Applications
- Mycorrhizal Fungi and Plant Interactions
- Bacteriophages and microbial interactions
Wuhan Polytechnic University
2024
Gansu Academy of Sciences
2015-2024
Lanzhou University
2015-2016
To obtain co‐cultures of anaerobic fungi and their indigenously associated methanogens from the rumen yaks grazing on Qinghai‐Tibetan Plateau investigate morphology features ability to degrade lignocellulose. Twenty fungus‐methanogen were obtained by Hungate roll‐tube technique. The identified as Orpinomyces, Neocallimastix Piromyces genera based morphological characteristics internal transcribed spacer 1 sequences analysis. All Methanobrevibacter sp. 16S rRNA gene sequencing. There four...
β-glucosidase derived from microorganisms has wide industrial applications. In order to generate genetically engineered bacteria with high-efficiency β-glucosidase, in this study two subunits (bglA and bglB) of obtained the yak rumen were expressed as independent proteins fused lactic acid (Lactobacillus lactis NZ9000). The strains L. NZ9000/pMG36e-usp45-bglA, NZ9000/pMG36e-usp45-bglB, NZ9000/pMG36e-usp45-bglA-usp45-bglB successfully constructed. These showed secretory expression BglA, BglB,...
Endoglucanase (EG) and cellobiohydrolase (CBH) which produced by microorganisms, have been widely used in industrial applications. In order to construct recombinant bacteria that produce high activity EG CBH, this study, eg (endoglucanase) cbh (cellobiohydrolase) were cloned from the rumen microbial genome of yak subsequently expressed independently co-expressed within Lactococcus lactis NZ9000 (L. NZ9000). The strains L. NZ9000/pMG36e-usp45-cbh lactis-cbh), NZ9000/pMG36e-usp45-eg...
Anaerobic fungi reside in the gut of herbivore and synergize with associated methanogenic archaea to decompose ingested plant biomass. Despite their potential for use bioconversion industry, only a few natural fungus-methanogen co-cultures have been isolated characterized. In this study we identified three Piromyces Methanobrevibacter ruminantium from rumen yaks grazing on Qinghai Tibetan Plateau. The representative co-culture, namely (Piromyces + M. ruminantium) Yak-G18, showed remarkable...
Abstract Anaerobic fungus–methanogen co-cultures from rumen liquids and faeces can degrade lignocellulose efficiently. In this study, 31 were first obtained the of yaks grazing in Qinghai Province, China, using Hungate roll-tube technique. The fungi identified according to morphological characteristics internal transcribed spacer (ITS) sequences. methanogens associated with each fungus by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) 16S rRNA gene sequencing....
Biorefinery using microorganisms to produce biofuels and value-added biochemicals derived from renewable biomass offers a promising alternative meet our sustainable energy environmental goals. The ethanologenic strain Zymomonas mobilis is considered as an excellent chassis for constructing microbial cell factories diverse due its outstanding industrial characteristics in ethanol production, high specific productivity, Generally Recognized Safe (GRAS) status. Nonetheless, the restricted...
OBJECTIVE: The experiment attempts to study the antioxidant activity in different parts of Gardenia jasminoides including roots, stems, leaves, branches, flowers and fruits explore development utilization new medicinal jasminoides. METHODS: Apply microwave-assisted extraction( MAE) extract total flavonoids from fruits, stems register ethanol concentration, extraction temperature time extraction.RESULT: polyphenols content was arranged high low as: flowers, roots; Branches, flowers; DPPH...
The clustered regularly interspaced short palindromic repeat (CRISPR) is an adaptive immune system that defends most archaea and many bacteria from foreign DNA, such as phages, viruses, plasmids. link between the CRISPR-Cas optimum growth temperature of thermophilic remains unclear. To investigate relationship structural characteristics, diversity, distribution properties in bacteria, genomes 61 species with complete genome sequences were downloaded GenBank this study. We used CRISPRFinder...