A5014189057- Prion Diseases and Protein Misfolding
- Natural Language Processing Techniques
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Topic Modeling
- 3D Printing in Biomedical Research
- Neurological diseases and metabolism
- Cancer Cells and Metastasis
- Glaucoma and retinal disorders
- Trace Elements in Health
- HIV/AIDS Research and Interventions
- Retinal Diseases and Treatments
- HIV Research and Treatment
- Reproductive tract infections research
- Alzheimer's disease research and treatments
- Microfluidic and Capillary Electrophoresis Applications
- Adolescent Sexual and Reproductive Health
- Erythrocyte Function and Pathophysiology
- Vascular Malformations Diagnosis and Treatment
- Sexual Differentiation and Disorders
- Reading and Literacy Development
- Text Readability and Simplification
- RNA regulation and disease
- Ocular Surface and Contact Lens
- Extracellular vesicles in disease
The Wallace H. Coulter Department of Biomedical Engineering
2018-2023
Georgia Institute of Technology
2018-2023
Vanderbilt University
2022-2023
Emory University
2020-2021
University of Southern California
2020
United States Air Force Research Laboratory
2015-2018
Provincial Health Services Authority
2018
Indiana University – Purdue University Indianapolis
1991-2016
Indiana University School of Medicine
1991-2016
MIT Lincoln Laboratory
2015
Abstract We sequenced the prion protein gene and studied biochemical characteristics intracerebral distribution of protease‐resistant with Western blot immunohistochemistry in 19 cases sporadic Creutzfeldt‐Jakob disease. identified four groups subjects defined by genotype at codon 129 gene, site a common methioninelvaline polymorphism, two types proteins that differed size glycosylation. The disease showed distinct clinicopathological features corresponded to previously described variants....
Current research has shown that comprehension can vary based on text and question types, readers' word recognition background knowledge may account for these differences. Other reader characteristics such as semantic syntactic awareness, inferencing, planning/organizing have also all been linked to reading comprehension, but not examined with regard specific types. The aim of this study was explore the relationships between characteristics, in children aged 10-14. We sought compare...
Gerstmann-Sträussler-Scheinker disease (GSS), a cerebello-pyramidal syndrome associated with dementia and caused by mutations in the prion protein gene (PRNP), is phenotypically heterogeneous. The molecular mechanisms responsible for such heterogeneity are unknown. Since we hypothesize that (PrP) may be clinico-pathologic heterogeneity, aim of this study was to analyze PrP several GSS variants. Among pathologic phenotypes GSS, recognize those without marked spongiform degeneration. In latter...
Vaginal microbicides for the prevention of HIV transmission may be an important option protecting women from infection. Incorporation dapivirine, a lead candidate nonnucleoside reverse transcriptase inhibitor, into intravaginal rings (IVRs) sustained mucosal delivery increase microbicide product adherence and efficacy compared with conventional vaginal formulations. Twenty-four healthy HIV-negative 18-35 years age were randomly assigned (1:1:1) to dapivirine matrix IVR, reservoir or placebo...
Glucocorticoids are widely used as an ophthalmic medication. A common, sight-threatening adverse event of glucocorticoid usage is ocular hypertension, caused by dysfunction the conventional outflow pathway. We report that netarsudil, a rho-kinase inhibitor, decreased glucocorticoid-induced hypertension in patients whose intraocular pressures were poorly controlled standard medications. Mechanistic studies our established mouse model show netarsudil both prevented and reduced pressure...
Understanding the link between vaccine immunogenicity and efficacy is currently a major focus in HIV research. Consequently, recent developments HIV-1 field have led to closer look at immune responses known efficacious vaccines. We undertook study explore clinical predictors of following recombinant hepatitis B (rHBV) vaccination cohort HIV-uninfected, virus naïve women living peri-urban setting Cape Town. Our aim was define host biological risk factors associated with lack uptake. found...
Diabetes mellitus is a complex metabolic disorder that associated with an increased risk of breast cancer. Despite this correlation, the interplay between tumor progression and diabetes, particularly regard to stiffening extracellular matrix, still mechanistically unclear. Here, we established murine model where hyperglycemia was induced before development. Using model, in vitro systems, patient samples, show increases growth, matrix stiffness, glycation, epithelial-mesenchymal transition...
Alternative RNA processing of human calcitonin/CGRP pre-mRNA is regulated by an intronic enhancer element. Previous studies have demonstrated that multiple sequence motifs within the and a number trans-acting factors play critical roles in regulation. Here, we report identification TIAR as novel player regulation alternative processing. binds to U tract motif downstream pseudo 5′ splice site previously characterized intron Binding promotes inclusion 3′-terminal exon located more than 200...
Cells respond to mechanical forces by deforming in accordance with viscoelastic solid behavior. Studies of microscale cell deformation observed high speed video microscopy have elucidated a new behavior which sufficiently rapid compression cells can lead transient volume loss and then recovery. This work has discovered that the resulting exchange between interior surrounding fluid be utilized for efficient, convective delivery large macromolecules (2000 kDa) interior. However, many...
Gerstmann-Sträussler-Scheinker (GSS) disease is a cerebral prion protein (PrP) amyloidosis associated with mutations in the PrP gene (PRNP). A GSS variant mutation at codon 198 (F198S) has been studied large Indiana kindred. Biochemical investigations showed that amyloid consists of 11 and 7 kDa fragments PrP. Immunohistochemical studies addition to amyloid, these patients accumulate deposits which are neither fluorescent nor birefringent when stained thioflavin S Congo red. In present...
Cancer cell migration is highly heterogeneous, and the migratory capability of cancer cells thought to be an indicator metastatic potential. It becoming clear that a does not have inherently metastasize, with weakly often found metastatic. However, mechanism through which escape from primary tumor remains unclear. Here, utilizing phenotypically sorted human breast cells, we demonstrate disseminate via communication stromal cells. While are capable single migration, rely on cell-cell...
The highly proliferative and pluripotent characteristics of embryonic stem cells engender great promise for tissue engineering regenerative medicine, but the rapid identification isolation target cell phenotypes remains challenging. Therefore, objectives this study were to characterize mechanics as a function differentiation employ differences in stiffness select population subsets with distinct mechanical, morphological, biological properties. Biomechanical analysis atomic force microscopy...
We present two patients with Gerstmann-Sträussler-Scheinker disease (GSS), one from a previously undescribed kindred and the Canadian branch of reported British kindred. In both patients, GSS is caused by substitution thymine for cytosine at codon 102 prion protein gene (PRNP). each patient, we confirmed clinical diagnosis neuropathologic examination. The mutation, causing leucine proline residue (P102L) protein, has been in least 30 other families. described here, mutation was coupling...
Stiffness has been observed to decrease for many cancer cell types as their metastatic potential increases. Although mechanics and are related, the underlying molecular factors associated with these phenotypes remain unknown. Therefore, we have developed a workflow measure mechanical properties gene expression of single cells that is used generate large linked-datasets. The process combines atomic force microscopy individual multiplexed RT-qPCR analysis on same cells. Surprisingly, genes...
Lung adenocarcinoma (LUAD) is the predominant type of lung cancer in U.S. and exhibits a broad variety behaviors ranging from indolent to aggressive. Identification biological determinants LUAD behavior at early stages can improve existing diagnostic treatment strategies. Extracellular matrix (ECM) remodeling cancer-associated fibroblasts play crucial role regulation aggressiveness there growing need investigate their determination stages. We analyzed tissue samples isolated patients with...
Abstract We present 3 patients with otopalatodigital (OPD) syndrome type II and omphalocele; 2 of the cases are brothers. There now 6 known OPD I or omphalocele. propose that this combination is not co‐incidental discuss mechanisms may result in OPD, omphalocele, other midline defects. © 1993 Wiley‐Liss, Inc.
Because of the acute nature most neurological events, families are often faced with rapid processing illness little time to realize that their loved one is going die.Watching dying process patients in care settings can be unsettling for both patients’ and staff. The neurocritical unit at Riverside Methodist Hospital Columbus, Ohio, wanted offer help traveling this difficult path. To support goal, an organized, intentional, flexible end-of-life intervention was needed. Using subjective data...