A5024114844- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Tissue Engineering and Regenerative Medicine
- Ubiquitin and proteasome pathways
- Heat shock proteins research
- Redox biology and oxidative stress
- Cancer-related gene regulation
- Cardiac Fibrosis and Remodeling
- Electrospun Nanofibers in Biomedical Applications
- Protein Structure and Dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Image Analysis Techniques
- Protein Degradation and Inhibitors
University of Aveiro
2018-2022
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2018-2022
Universidade do Porto
2018-2022
Deregulation of tRNAs, aminoacyl-tRNA synthetases and tRNA modifying enzymes are common in cancer, raising the hypothesis that protein synthesis efficiency accuracy (mistranslation) compromised tumors. We show here human colon tumors xenograft produced mice by two epithelial cancer cell lines mistranslate 2- to 4-fold more frequently than normal tissue. To clarify if mistranslation plays a role tumor biology, we expressed mutant Ser-tRNAs misincorporate Ser-at-Ala (frequent error) Ser-at-Leu...
Protein conformational disorders are characterized by disruption of protein folding and toxic accumulation aggregates. Here we describe a sensitive simple method to follow monitor general aggregation in human cells. Heat shock 27 (HSP27) is an oligomeric small heat that binds keeps unfolded proteins competent state. This high specificity HSP27 for aggregated can be explored living cells fusing it fluorescent as Green Fluorescent (GFP). We have constructed HeLa stable cell line expressing...
Deregulation of tRNAs, aminoacyl-tRNA synthetases (aaRS) or tRNA modifying enzymes, increase the level protein synthesis errors (PSE) and are associated with several diseases, but cause-effect mechanisms these pathologies remain elusive. To clarify role PSE in human biology, we have engineered a HEK293 cell line to overexpress wild type (Wt) tRNASer two mutants that misincorporate serine at non-cognate codon sites. Then, followed long-term adaptation such recombinant cells by analysing...
Serine tRNAs (tRNASer) are frequently overexpressed in tumors and associated with poor prognosis increased risk of recurrence breast cancer. Impairment tRNA biogenesis abundance also impacts proteome homeostasis, activates protein quality control systems. Herein, we aimed at testing whether increasing tRNASer could foster tumor establishment through activation the UPR. In order to do so, firstly confirmed that expression tRNA-Ser-AGA-2-1 [hereafter tRNASer(AGA)] was upregulated by 1.79-fold...