A5015314964- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- Ion channel regulation and function
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Hearing, Cochlea, Tinnitus, Genetics
- Neuroinflammation and Neurodegeneration Mechanisms
- Biotin and Related Studies
- Molecular Sensors and Ion Detection
- Ion Transport and Channel Regulation
- Anesthesia and Neurotoxicity Research
- Barrier Structure and Function Studies
- Intensive Care Unit Cognitive Disorders
Heinrich Heine University Düsseldorf
2020-2025
University of Kaiserslautern
2019-2020
Astrocytic gap junctional coupling is a major element in neuron–glia interaction. There strong evidence that impaired involved neurological disorders. Reduced was, e.g., demonstrated for core regions of ischemic stroke suffer from massive cell death. In the surrounding penumbra, cells may recover, but recovery hampered by spreading depolarizations, which impose additional metabolic stress onto tissue. Spreading depolarizations are characterized transient breakdown cellular ion homeostasis,...
Ischemic conditions cause an increase in the sodium concentration of astrocytes, driving breakdown ionic homeostasis and exacerbating cellular damage. Astrocytes express high levels electrogenic sodium-bicarbonate cotransporter1 (NBCe1), which couples intracellular Na+ to regulation pH operates close its reversal potential under physiological conditions. Here, we analyzed mode operation during transient energy deprivation via imaging astrocytic pH, Na+, ATP organotypic slice cultures mouse...
Malfunction of astrocytic K+ regulation contributes to the breakdown extracellular homeostasis during ischemia and spreading depolarization events. Studying astroglial changes is, however, hampered by a lack suitable techniques. Here, we combined results from fluorescence imaging, ion-selective microelectrodes, patch-clamp recordings in murine neocortical slices with calculation [K+]. Brief chemical caused reversible ATP reduction transient astrocytes. Moreover, [Na+] increased 24 mM...
Anisotropy of tracer-coupled networks is a hallmark in many brain regions. In the past, topography these was analyzed using various approaches, which focused on different aspects, e.g., position, tracer signal, or direction coupled cells. Here, we developed vector-based method to analyze extent and preferential spreading. As model region, chose lateral superior olive—a nucleus that exhibits specialized network topography. acute slices, sulforhodamine 101-positive astrocytes were...
Anisotropic gap junctional coupling is a distinct feature of astrocytes in many brain regions. In the lateral superior olive (LSO), astrocytic networks are anisotropic and oriented orthogonally to tonotopic axis. CaV1.3 knock-out (KO) otoferlin KO mice, where auditory brainstem nuclei deprived from spontaneous cochlea-driven neuronal activity, circuitry disturbed. So far it was unknown if this disturbance also accompanied by an impaired topography LSO astrocyte networks. To answer question,...
Fluorescence lifetime imaging microscopy (FLIM) is a highly valuable technique in the fluorescence toolbox because it essentially independent of indicator concentrations. Conventional analyzes changes emission intensity. In contrast, FLIM assesses lifetime, which defined as time fluorophore remains an excited state before emitting photon. This principle advantageous experiments where concentrations are expected to change, e.g., due cell volume. FLIM, however, requires collecting substantial...