A5009617977- Microbial Community Ecology and Physiology
- Marine Biology and Ecology Research
- Crustacean biology and ecology
- Genomics and Phylogenetic Studies
- Coral and Marine Ecosystems Studies
- Parasite Biology and Host Interactions
- Insect symbiosis and bacterial influences
- Protist diversity and phylogeny
- Marine Bivalve and Aquaculture Studies
- Environmental DNA in Biodiversity Studies
- Methane Hydrates and Related Phenomena
- Aquatic Invertebrate Ecology and Behavior
- Prion Diseases and Protein Misfolding
- Insect Utilization and Effects
- Aquaculture Nutrition and Growth
- Research Data Management Practices
- Mycorrhizal Fungi and Plant Interactions
- Ichthyology and Marine Biology
- Marine Biology and Environmental Chemistry
- Enzyme Production and Characterization
- Analytical chemistry methods development
- Bacterial Genetics and Biotechnology
- Invertebrate Immune Response Mechanisms
- Coastal wetland ecosystem dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Ifremer
2016-2025
Université de Bretagne Occidentale
2016-2025
Centre National de la Recherche Scientifique
2011-2024
Biology of Extremophiles Laboratory
2004-2022
Institut Universitaire Européen de la Mer
2016-2018
Station Biologique de Roscoff
2011
Sorbonne Université
2011
Biologie Intégrative des Organismes Marins
2001
Abstract Despite representing one of the largest biomes on earth, biodiversity deep seafloor is still poorly known. Environmental DNA metabarcoding offers prospects for fast inventories and surveys, yet requires standardized sampling approaches careful choice environmental substrate. Here, we aimed to optimize genetic assessment prokaryote (16S), protistan (18S V4), metazoan V1–V2, COI) communities, by evaluating strategies sediment aboveground water, deployed simultaneously at deep-sea...
A thermophilic, anaerobic, chemo-organotrophic bacterium, designated MV1087T, was isolated from a deep-sea hydrothermal chimney sample collected the Mid-Atlantic Ridge. The cells were straight, motile and stained gram-negative. Growth observed 45 to 65 degrees C, with an optimum around C. No growth at 40 or 70 pH 5.5 9.0 7. salinity range for 10-100 g sea salt l(-1) (corresponding 6.5-65 NaCl l(-1)) 30 (20 l(-1)). Strain MV1087T heterotrophic, able ferment proteinaceous substrates, such as...
A novel thermophilic, anaerobic, hydrogen-oxidizing bacterium, designated strain AM1116T, was isolated from an East Pacific Rise hydrothermal vent sample. The cells were rod-shaped (1.01-5 x 0.5 microm), motile with polar flagella. They grew at temperatures between 50 and 70 degrees C (optimum 60 C; doubling time approximately 1.5 h), pH 5.0 7.5 around 5.5-6.0) in 10 40 g NaCl l(-1) 20-25 l(-1)). Cells chemolithoautotrophically a H2/CO2 atmosphere (80:20; 200 kPa). Poor heterotrophic growth...
Rimicaris exoculata dominates the megafauna of several Mid-Atlantic Ridge hydrothermal sites. Its gut is full sulphides and iron-oxide particles harbours microbial communities. Although a trophic symbiosis has been suggested, their role remains unclear. In vivo starvation experiments in pressurized vessels were performed on shrimps from Rainbow Trans-Atlantic Geotraverse sites order to expel transient contents. Microbial communities associated with starved reference compared using 16S rRNA...
A thermophilic, non-spore-forming, marine, sulfate-reducing bacterium, strain SL6T, was isolated from deep-sea hydrothermal sulfides collected at Guaymas Basin. The gram-negative-staining cells occurred singly or in pairs as small, highly motile rods. temperature range for growth 50-80 degrees C with an optimum 75 C. pH 70 6.3-6.8, 6.5. NaCl concentration 5-55 g l(-1), 30 l(-1). H2 and CO2 were the only substrates sulfate reduction. However, stimulated by several organic compounds. Sulfur,...
Abstract Background Free-living and symbiotic chemosynthetic microbial communities support primary production higher trophic levels in deep-sea hydrothermal vents. The shrimp Rimicaris exoculata , which dominates animal along the Mid-Atlantic Ridge, houses a complex bacterial community its enlarged cephalothorax. dominant bacteria present are from taxonomic groups Campylobacteria Desulfobulbia (formerly Deltaproteobacteria ), Alphaproteobacteria Gammaproteobacteria some recently discovered...
ABSTRACT Accessing the metabolic functioning of deep‐sea animals in situ remains a technological challenge as recovery time samples is incompatible with short lifespan such molecules mRNAs. Tools able to preserve RNA exist, but they are study mobile fauna. Here, we describe new sampling tool, named FISH (fixer homogenized substrates), implemented on submersible and equipped number specific features collect tissue Connected suction pump submersible, sampler incorporates bowl which two bottles...
Sea-floor massive sulfide deposits (SMS) harbor over 108 tons of mineral resources along the mid-ocean ridges representing a significant polymetallic reservoir [1]. These SMS are mainly composed iron and sulfur, but also contain copper, zinc, gold, silver rare-earth elements, making them attractive for mining industry. Little is known about geo-biodiversity ecosystem services associated with these especially inactive or extinct ones. Deep dark chemosynthetic microbial ecosystems deep-sea...
Within the deep ocean, hydrothermal vent ecosystems are home to unique set of species' communities, that live nowhere else, and whose food chains have particularity rely on microorganisms activities through a process called chemosynthesis. Many animals living there adopted strategy hosting these in symbiosis or within their body, including many most emblematic groups such as tubeworms, Pompeii worm, yeti "crabs" scaly-foot hairy snails. Among them, shrimps Alvinocarididae family constitute...
Transmissible spongiform encephalopathies are caused by accumulation of highly resistant misfolded amyloid prion protein PrPres and can be initiated penetration such pathogen molecules from infected tissue to intact organism. Decontamination animal meal containing is proposed thanks the use proteolytic enzymes secreted thermophilic bacteria Thermoanaerobacter, Thermosipho, Thermococcus subsp. mesophilic soil Streptomyces Keratins α β, which resemble structures, were used as substrates for...
Rimicaris exoculata is a deep-sea hydrothermal vent shrimp whose enlarged gill chamber houses complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial This species dominates ecosystem megafauna along the Mid-Atlantic Ridge, regardless of contrasting geochemical conditions prevailing in them. Here, resident epibiont community at four contrasted sites (Rainbow, TAG, Logatchev Ashadze) was analysed compiled with previous data to evaluate possible influence...
Rimicaris exoculata is one of the most well-known and emblematic species endemic vent fauna. Like many other from these ecosystems, shrimps host important communities chemosynthetic bacteria living in symbiosis with their inside cephalothorax gut. For symbiotic partners, mode transmission remains to be elucidated starting point relationship not yet defined, but could begin egg. In this study, we explored proliferation microbial on R. broods through embryonic development using a combination...
Rimicaris chacei Williams and Rona 1986, formerly named as Chorocaris chacei, is a caridean shrimp living in deep-sea hydrothermal ecosystems. This endemic to the Mid Atlantic Ridge (MAR) lives at periphery of aggregates its well-known congeneric R. exoculata 1986. Contrasting with very dense mobile clusters formed by exoculata, small groups several individuals that are not mobile. Although devoid characteristic hypertrophied cephalothorax which harbors ectosymbionts, microbial community has...
The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting active smokers of deep-sea mid Atlantic ridge vent fields. We investigated nature host mechanisms controlling vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. belongs to Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in exoculata. Re-crustin is mainly...
Abstract Here we report the discovery of a high-temperature hydrothermal vent field on Woodlark Ridge, using ship-borne multibeam echosounding and Remotely Operated Vehicle (ROV) exploration. La Scala Vent Field comprises two main active areas several inactive zones dominated by variably altered basaltic rocks, indicating that an stable circulation has been maintained over long period time. The Pandora Site, at depth 3380 m, is mainly composed diffuse vents. Corto site, 3360 characterized...
Deep-sea hydrothermal vent meiofauna have been the focus of recent research and discovery an abundant well-adapted free-living marine nematode on Mid-Atlantic Ridge offers new perspectives adaptations to environment. Indeed, knowledge concerning biological interactions microbes in extreme environments is scarce, especially for nematodes. In this study, we used microscopic observations [fluorescence situ hybridization (FISH) scanning electron microscopy (SEM)] metabarcoding 16S rRNA...
The mechanisms underpinning the replication of genomic DNA have recently been challenged in
Abstract Molluscs have undergone many transitions between separate sexes and hermaphroditism, which is of interest in studying the evolution sex determination differentiation. Here, we combined multi-locus genotypes obtained from restriction site-associated DNA (RAD) sequencing with anatomical observations gonads three deep-sea hydrothermal vent gastropods genus Alviniconcha living southwest Pacific. We found that all species (Alviniconcha boucheti, strummeri, kojimai) share same...
A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3T was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain possessed few laterally inserted flagella, had DNA G+C content 33·1 mol% grew optimally pH 6·6 45 °C. Growth observed temperatures between 20 58 °C values 5·8 8·2. The optimum NaCl concentration for growth 3 % sea salt (30 g l−1); no in presence 15...
A novel anaerobic, Gram-positive, sporulating and strictly chemoorganoheterotrophic bacterium, designated strain DV1184 T , was isolated from a deep-sea hydrothermal vent sample the Mid-Atlantic Ridge. The cells were short, straight rods (4 μm long 0·8 wide) motile with peritrichous flagella. They grew between 35 55 °C (optimum, 45 °C), pH 5·0 8·0 6·0) at 20–60 g sea salts l −1 (optimum concentration, 30 ). Strain able to ferment yeast extract, tryptone, peptone, glucose, sucrose, maltose...