A5020236088- Immune Response and Inflammation
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Autoimmune Bullous Skin Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Extracellular vesicles in disease
- Advanced Fluorescence Microscopy Techniques
- Mast cells and histamine
- Coenzyme Q10 studies and effects
- Myasthenia Gravis and Thymoma
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Mitochondrial Function and Pathology
- Autoimmune Neurological Disorders and Treatments
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Peripheral Neuropathies and Disorders
- Cancer, Hypoxia, and Metabolism
- Advanced Chemical Sensor Technologies
- Asthma and respiratory diseases
- Lymphatic System and Diseases
- Cellular Mechanics and Interactions
- Immunodeficiency and Autoimmune Disorders
- Systemic Sclerosis and Related Diseases
- Redox biology and oxidative stress
Leiden University Medical Center
2023
Radboud University Medical Center
2016-2021
Radboud Institute for Molecular Life Sciences
2016-2021
Radboud University Nijmegen
2016-2021
University Medical Center
2016-2017
Abstract Dendritic cells (DCs) present foreign antigen in major histocompatibility complex (MHC) class I molecules to cytotoxic T a process called cross-presentation. An important step this is the release of from lumen endosomes into cytosol, but mechanism still unclear. In study, we show that reactive oxygen species (ROS) produced by NADPH-oxidase NOX2 cause lipid peroxidation, membrane disrupting chain-reaction, which turn results leakage endosomes. Antigen and cross-presentation were...
Abstract Podosomes are cytoskeletal structures crucial for cell protrusion and matrix remodelling in osteoclasts, activated endothelial cells, macrophages dendritic cells. In these hundreds of podosomes spatially organized diversely shaped clusters. Although we others established individual as micron-sized mechanosensing protrusive units, the exact scope spatiotemporal organization podosome clustering remain elusive. By integrating a newly developed extension Spatiotemporal Image Correlation...
Neutrophils kill ingested pathogens by the so-called oxidative burst, where reactive oxygen species (ROS) are produced in lumen of phagosomes at very high rates (mM/sec), although these can only be maintained for a short period (minutes). In contrast, dendritic cells produce ROS much lower rates, but they sustain production longer after pathogen uptake (hours). It is becoming increasingly clear that this slow prolonged essential antigen cross-presentation to activate cytolytic T and shapes...
Cross-presentation of foreign antigen in major histocompatibility complex (MHC) class I by dendritic cells (DCs) requires activation the NADPH-oxidase NOX2 complex. We recently showed that is recruited to phagosomes SNARE protein VAMP8 where NOX2-produced reactive oxygen species (ROS) cause lipid oxidation and membrane disruption, promoting translocation into cytosol for cross-presentation. In this study, we extend these findings showing also involved trafficking endosomes. Moreover,...
Dendritic cells (DCs) constantly sample peripheral tissues for antigens, which are subsequently ingested to derive peptides presentation T in lymph nodes. To do so, DCs have traverse many different with varying oxygen tensions. Additionally, often exposed low tensions tumors, where vascularization is lacking, as well inflammatory foci, rapidly consumed by during the respiratory burst. respond levels tailor immune responses such low-oxygen environments. In present study, we identified a...
Ethylene is a major plant hormone mediating developmental processes and stress responses to stimuli such as infection. We show here that ethylene also produced during systemic inflammation in humans released exhaled breath. Traces of were detected by laser spectroscopy both vitro isolated blood leukocytes exposed bacterial lipopolysaccharide (LPS) well vivo following LPS administration healthy volunteers. Exposure triggers formation product lipid peroxidation induced the respiratory burst....
SSc is a complex disease characterized by vascular abnormalities and inflammation culminating in hypoxia excessive fibrosis. Previously, we identified chemokine (C-X-C motif) ligand 4 (CXCL4) as novel predictive biomarker SSc. Although CXCL4 well-studied, the mechanisms driving its production are unclear. The aim of this study was to elucidate leading production.Plasmacytoid dendritic cells (pDCs) from 97 healthy controls 70 patients were cultured presence or atmospheric oxygen level and/or...
Cells are exposed to reactive oxygen species (ROS) as a by-product of mitochondrial metabolism, especially under hypoxia. ROS also enzymatically generated at the plasma membrane during inflammation. Radicals cause cellular damage leading cell death, they react indiscriminately with surrounding lipids, proteins, and nucleotides. However, important for many physiological processes, including signaling, pathogen killing chemotaxis. The sensitivity cells therefore likely depends on subcellular...
Abstract A subset of autoimmune diseases is characterized by predominant pathogenic IgG4 autoantibodies (IgG4-AIDs). Why predominates in these disorders unknown. We hypothesized that dysregulated B cell maturation or aberrant class switching causes overrepresentation + cells and plasma cells. Therefore, we compared the compartment patients with muscle-specific kinase (MuSK) myasthenia gravis (MG), pemphigus, leucine-rich glioma inactivated (LGI1) encephalitis contactin-associated...
A subset of autoimmune diseases is characterized by predominant pathogenic IgG4 autoantibodies (IgG4-AID). Why predominates in these disorders unknown. We hypothesized that dysregulated B cell maturation or aberrant class switching causes overrepresentation