A5100329740- Bacteriophages and microbial interactions
- Biosensors and Analytical Detection
- Bacterial Infections and Vaccines
- Monoclonal and Polyclonal Antibodies Research
- Microfluidic and Bio-sensing Technologies
- Immunotherapy and Immune Responses
- Pneumonia and Respiratory Infections
- HIV Research and Treatment
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Heat shock proteins research
- Plant Pathogenic Bacteria Studies
- Transgenic Plants and Applications
- Cell death mechanisms and regulation
- Bacillus and Francisella bacterial research
- Insects and Parasite Interactions
- Influenza Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- interferon and immune responses
- Nitric Oxide and Endothelin Effects
- Microfluidic and Capillary Electrophoresis Applications
- Glycosylation and Glycoproteins Research
National Chung Hsing University
2012-2024
National Cheng Kung University
2018
National Yang Ming Chiao Tung University
2003-2014
Boston University
2013-2014
National Taipei University
2013
National Health Research Institutes
2008-2012
Symphogen (Denmark)
2006
Kaohsiung Chang Gung Memorial Hospital
1998
Emory University
1995-1997
The integration of novel surface‐enhanced Raman scattering (SERS) nanoprobes and a microfluidic dielectrophoresis (DEP) device is developed for rapid on‐line SERS detection Salmonella enterica serotype Choleraesuis Neisseria lactamica . are prepared by immobilization specific antibody onto the surface nanoaggregate‐embedded beads (NAEBs), which silica‐coated, dye‐induced aggregates small number gold nanoparticles (AuNPs). Each NAEB gives highly enhanced signals owing to presence well‐defined...
We describe the expression and consistent production of a first target-specific recombinant human polyclonal antibody. An anti-Rhesus D antibody, Sym001, comprised 25 unique IgG1 antibodies, was produced by novel Sympress technology. This strategy is based on site-specific integration antibody genes in CHO cells, using FRT/Flp-In recombinase system. allows construct at same genomic site host thereby reducing position effects. Different bioreactor batches Sym001 displayed highly manufacturing...
Luteolin (3',4',5,7-tetrahydroxyflavone) is a common flavonoid in many types of plants and has several beneficial biological effects, including anti-inflammation, anti-oxidant, anti-cancer properties. However, the detail mechanisms luteolin suppressing tumor invasion metastasis are poorly understood. Here, we investigated effects on glioblastoma cell migration activity. Under non-cytotoxic doses (15 30 μM), exhibited an inhibitory effect U-87 MG T98G cells. Additionally, filopodia assembly...
ABSTRACT Helicobacter pylori is a common human pathogen that has been identified to be carcinogenic. This study isolated the temperate bacteriophage 1961P from lysate of clinical strain H. in Taiwan. The an icosahedral head and short tail, typical Podoviridae family. Its double-stranded DNA genome 26,836 bp long 33 open reading frames. Only 9 predicted proteins have homologs known functions, while remaining 24 are only similar unknown encoded by prophages remnants. Analysis sequences...
The envelope proteins of human immunodeficiency virus (HIV) and simian (SIV) were found to be modified by fatty acylation the transmembrane protein subunit gp41. precursor gp160 was also palmitoylated prior its cleavage into gp120 gp41 subunits. palmitic acid label sensitive treatment with hydroxylamine or 2-mercaptoethanol, indicating that linkage is through a thioester bond. Treatment cycloheximide did not prevent incorporation [3H]palmitic HIV protein, palmitoylation posttranslation...
Antimicrobial resistance (AMR) has become a crucial global health issue. Antibiotic-resistant bacteria can survive after antibiotic treatments, lowering drug efficacy and increasing lethal risks. A microfluidic water-in-oil emulsion droplet system entrap microorganisms antibiotics within the tiny bioreactor, separate from surroundings, enabling independent assays that be performed in high-throughput manner. This study presents development of label-free dielectrophoresis (DEP)-based platform...
It was previously reported that truncation or proteolytic removal of the C-terminal 16 amino acids (the R peptide) from cytoplasmic tail murine leukemia virus (MuLV) envelope protein greatly increases its fusion activity. In this study, to investigate specificity effect peptide on activity viral proteins, we expressed simian immunodeficiency (SIV)-MuLV chimeric proteins in which entire SIV replaced by either full-length MuLV a truncated with deleted. Extensive CD4-positive cells containing...
In previous studies, the C-terminal R peptide of murine leukemia virus (MuLV) Env protein was shown to be a potent inhibitor viral fusion activity. present study, we investigated molecular determinants in MuLV cytoplasmic tail which are important for inhibition activity peptide. We constructed series mutant env genes express proteins with serial truncations, internal deletions, or amino acid substitutions tail. To analyze their cell activity, employed quantitative assay. found that...
Abstract Many potential vaccine candidates for serogroup B Neisseria meningitidis (NMB) have been identified by reverse vaccinology, a genome‐based approach. However, some may be unseen owing to uncertain annotation or their peculiar properties. In this study, we describe the preparation and identification of novel lipoprotein expressed in all meningococcal strains tested. mAb were first prepared from mice immunized with strain isolated Taiwan. Total proteins immunizing separated 2‐DE...
Laryngospasm occurring after tracheal extubation in children is potentially dangerous. This study uses acupuncture with bloodletting at the Shao Shang (L 11) or Yang (Li 1) acupoints to investigate whether this technique can prevent treat laryngospasm. Seventy‐six patients were randomly divided into two groups. Patients group ( n = 38) treated bilateral acupunctures end of operation. control not. The incidence laryngospasm (5.3%) was less than that (23.7%) (p < 0.05). If developed,...
Abstract Background The blaB, blaGOB and blaCME genes are thought to confer β-lactam resistance Elizabethkingia anophelis, based on experiments conducted primarily Escherichia coli. Objectives To determine the individual contributions of β-lactamase increased MICs in E. anophelis assess their impact vivo efficacy carbapenem therapy. Methods Scarless gene deletion one or more gene(s) was performed three clinical isolates. were determined by broth microdilution. Hydrolytic activity expressions...
Treatment of advanced hepatocellular carcinoma (HCC) has exhibited a poor overall survival rate only six to ten months, and the urgency development more effective novel agents is ever present.In this line research, we aimed investigate effects inhibitive mechanisms aqueous Ocimum gratissimum leaf extract (OGE), gratissimum, which commonly used as therapeutic herb for its numerous pharmacological properties, on malignant HCC cells.Our results showed that OGE decreased cell viability SK-Hep1...
We aimed at investigating the expression of long non-coding RNA (lncRNA) Ptprj-as1 and role lncRNAPtprj-as1 in inflammatory cells after intracerebral hemorrhage its potential mechanism.The rat model was established. Expressions cytokines were detected by quantitative Real-time PCR (qRT-PCR). After BV2 transfected with lentivirus, cell proliferation, migrative ability apoptosis counting kit-8 (CCK-8) assay, transwell chamber flow cytometry, respectively. Immunofluorescence used to explore...
We have previously described two types of protective B-cell epitopes in the O-antigen (OAg) Gram-negative bacterium Francisella tularensis: repeating internal targeted by vast majority anti-OAg monoclonal antibodies (mAbs), and a non-overlapping epitope at non-reducing end unique IgG2a mAb FB11. now generated characterized three mAbs specific for F. tularensis OAg, partially encoded same variable region germline genes, indicating that they target epitope. Like FB11, new mAbs, Ab63 (IgG3),...