A5015858867- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Advanced Electron Microscopy Techniques and Applications
- RNA and protein synthesis mechanisms
- Plant Pathogens and Fungal Diseases
- Electron and X-Ray Spectroscopy Techniques
- Banana Cultivation and Research
- Plant Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Microbial Natural Products and Biosynthesis
- Agriculture, Plant Science, Crop Management
- Bacterial biofilms and quorum sensing
- Aquaculture disease management and microbiota
- Postharvest Quality and Shelf Life Management
- Cancer-related Molecular Pathways
- Herbal Medicine Research Studies
- Microfluidic and Bio-sensing Technologies
- Plant responses to water stress
- Microbial Community Ecology and Physiology
- Complementary and Alternative Medicine Studies
- Plant Growth Enhancement Techniques
- Cannabis and Cannabinoid Research
- Plant Pathogens and Resistance
- Diet and metabolism studies
Gladstone Institutes
2024-2025
University of California, Berkeley
2022-2024
University of California, San Diego
2017-2022
Stanford University
2020
University of Wisconsin–Madison
2016
Indian Institute of Technology Delhi
2012-2014
University of Allahabad
1978-1992
Botanical Survey of India
1986
We observed the assembly of a nucleus-like structure in bacteria during viral infection. Using fluorescence microscopy and cryo-electron tomography, we showed that Pseudomonas chlororaphis phage 201φ2-1 assembled compartment separated DNA from cytoplasm. The was centered by bipolar tubulin-based spindle, it segregated bacterial proteins according to function. Proteins involved replication transcription localized inside compartment, whereas translation nucleotide synthesis outside. Later...
Abstract Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction–modification and CRISPR–Cas systems 1 . In response, one family large bacteriophages uses a nucleus-like compartment to protect its replicating by excluding host defence factors 2–4 However, principal composition structure this remain unknown. Here we find bacteriophage nuclear shell assembles primarily from protein, which name chimallin (ChmA). Combining cryo-electron...
The Gram-positive bacterium Bacillus subtilis can divide via two modes. During vegetative growth, the division septum is formed at midcell to produce equal daughter cells. However, during sporulation, closer one pole yield a smaller forespore and larger mother cell. Using cryo-electron tomography, genetics fluorescence microscopy, we found that organization of machinery different in septa. While FtsAZ filaments, major orchestrators bacterial cell division, are present uniformly around...
Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to randomly throughout the host cell cytoplasm. Here, we show that viral of
The study of bacterial cell biology is limited by difficulties in visualizing cellular structures at high spatial resolution within their native milieu. Here, we visualize Bacillus subtilis sporulation using cryo-electron tomography coupled with cryo-focused ion beam milling, allowing the reconstruction native-state sections molecular resolution. During sporulation, an asymmetrically-positioned septum generates a larger mother and smaller forespore. Subsequently, engulfs We show that septal...
Abstract Since the emergence of deadly pathogens and multidrug-resistant bacteria at an alarmingly increased rate, bacteriophages have been developed as a controlling bioagent to prevent spread pathogenic bacteria. One these pathogens, disease-causing Vibrio parahaemolyticus (VP AHPND ) which induces acute hepatopancreatic necrosis, is considered one deadliest shrimp has recently become resistant various classes antibiotics. Here, we discovered novel vibriophage that specifically targets...
Upon infection of Pseudomonas cells, jumbo phages 201Φ2-1, ΦPA3, and ΦKZ assemble a phage nucleus. Viral DNA is enclosed within the phage-encoded proteinaceous shell along with proteins associated replication, recombination transcription. Ribosomes involved in metabolic processes are excluded from RNA synthesis occurs inside nucleus messenger presumably transported into cytoplasm to be translated. Newly synthesized either remain or specifically translocate The molecular mechanisms governing...
Abstract Coronavirus non‐structural protein 3 (nsp3) forms hexameric crowns of pores in the double membrane vesicle that houses replication–transcription complex. Nsp3 SARS‐like viruses has three unique domains absent other coronavirus nsp3 proteins. Two these, SUD‐N (Macrodomain 2) and SUD‐M 3), form two lobes connected by a peptide linker an interdomain disulfide bridge. We resolve first complete x‐ray structure SARS‐CoV SUD‐N/M as well mutant variant SARS‐CoV‐2 modified to restore...
Significance Targeting specific genomic loci with synthetic molecules remains a major goal in chemistry, biology, and precision medicine. Identifying how genome readers bind the chromatinized cells would facilitate their development, but doing so formidable challenge. We map genome-wide binding patterns for two structurally distinct molecules. To achieve this goal, we couple our cross-linking of small to isolate chromatin approach next-generation sequencing. In addition high-affinity sites,...
The threat to public health posed by drug-resistant bacteria is rapidly increasing, as some of healthcare’s most potent antibiotics are becoming obsolete. Approximately two-thirds the world’s derived from natural products produced Streptomyces encoded biosynthetic gene clusters. Thus, identify novel clusters, we sequenced genomes four bioactive strains isolated soil in San Diego County and used Bacterial Cytological Profiling adapted for agar plate culturing order examine mechanisms...
Bacteria encode myriad defenses that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR/Cas systems. In response, one family large bacteriophage employs a nucleus-like compartment to protect their replicating by excluding host defense factors. However, principle composition structure this remain unknown. Here, we find nuclear shell assembles primarily from protein, termed chimallin. Combining cryo-electron tomography shells in bacteriophage-infected...
ABSTRACT Coronavirus non-structural protein 3 (nsp3) forms hexameric crowns of pores in the double membrane vacuole that houses replication-transcription complex. Nsp3 SARS-like viruses has three unique domains absent other coronavirus nsp3 proteins. Two these, SUD-N (Macrodomain 2) and SUD-M 3), form two lobes connected by a peptide linker an interdomain disulfide bridge. We resolve first complete x-ray structure SARS-CoV SUD-N/M as well mutant variant SARS-CoV-2 modified to restore...
Abstract Upon infection of Pseudomonas cells, jumbo phages 201Φ2-1, ΦPA3, and ΦKZ assemble a phage nucleus. Viral DNA is enclosed within the phage-encoded proteinaceous shell along with proteins associated replication, recombination transcription. Ribosomes involved in metabolic processes are excluded from RNA synthesis occurs inside nucleus messenger presumably transported into cytoplasm to be translated. Newly synthesized either remain or specifically translocate The molecular mechanisms...
The mechanistic details of bacterial cell division are poorly understood. Gram-positive bacterium Bacillus subtilis can divide via two modes. During vegetative growth, the septum is formed at mid to produce equal daughter cells. However, during sporulation, closer one pole yield a smaller forespore and larger mother cell. We use cryo-electron tomography visualize architectural differences in organization FtsAZ filaments, major orchestrators these conditions. demonstrate that filaments...
species belonging to the pseudomallei group include significant human and animal pathogens as well non-pathogenic