A5057745202- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Parasitic Infections and Diagnostics
- CRISPR and Genetic Engineering
- Trypanosoma species research and implications
- Genomics and Rare Diseases
- Cancer-related gene regulation
- Bacterial Genetics and Biotechnology
Weizmann Institute of Science
2022-2025
Abstract Trypanosomes are protozoan parasites that cycle between insect and mammalian hosts the causative agent of sleeping sickness. Here, we describe changes pseudouridine (Ψ) modification on rRNA in two life stages parasite using four different genome-wide approaches. CRISPR-Cas9 knock-outs all snoRNAs guiding Ψ helix 69 (H69) large subunit were lethal. A single knock-out a snoRNA Ψ530 H69 altered composition 80S monosome. These specifically affected translation only subset proteins. This...
Giardiasis is a disease caused by the protist Giardia lamblia. As no human vaccines have been approved so far against it, and resistance to current drugs spreading, new strategies for combating giardiasis need be developed. The G. lamblia ribosome may provide promising therapeutic target due its distinct sequence differences from ribosomes of most eukaryotes prokaryotes. Here, we report cryo-electron microscopy structure (WB strain) determined at 2.75 Å resolution. ribosomal RNA shortest...
Ribosomes, the cellular organelles translating genetic code to proteins, are assemblies of RNA chains and many proteins (RPs) arranged in precise fine‐tuned interwoven structures. Mutated ribosomal genes cause ribosomopathies, including Diamond Blackfan anemia (DBA, a rare heterogeneous red‐cell aplasia connected ribosome malfunction) or failed biogenesis. Combined bioinformatical, structural, predictive analyses potential consequences possibly expressed mutations eS19, protein product...