A5062477256- Protein Structure and Dynamics
- Enzyme Structure and Function
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- Machine Learning in Bioinformatics
- Molecular spectroscopy and chirality
- RNA Research and Splicing
- Heat shock proteins research
- RNA modifications and cancer
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Bioinformatics and Genomic Networks
- Cholinesterase and Neurodegenerative Diseases
- Plant Reproductive Biology
- Bacteriophages and microbial interactions
- Neurological diseases and metabolism
- Toxin Mechanisms and Immunotoxins
- Biocrusts and Microbial Ecology
- Marine and coastal plant biology
- Computational Drug Discovery Methods
- Parkinson's Disease Mechanisms and Treatments
- Algal biology and biofuel production
- Cancer-related molecular mechanisms research
Eötvös Loránd University
2021-2025
HUN-REN Research Centre for Natural Sciences
2016-2022
Institute of Molecular Life Sciences
2016-2022
Hungarian Academy of Sciences
2016-2019
Széchenyi István University
2016
Intrinsically disordered proteins, defying the traditional protein structure-function paradigm, are a challenge to study experimentally. Because large part of our knowledge rests on computational predictions, it is crucial that their accuracy high. The Critical Assessment Intrinsic Disorder prediction (CAID) experiment was established as community-based blind test determine state art in intrinsically regions and subset residues involved binding. A total 43 methods were evaluated dataset 646...
The Database of Protein Disorder (DisProt, URL: www.disprot.org) has been significantly updated and upgraded since its last major renewal in 2007. current release holds information on more than 800 entries IDPs/IDRs, i.e. intrinsically disordered proteins or regions that exist function without a well-defined three-dimensional structure. We have re-curated previous to purge DisProt from conflicting cases, also the functional classification scheme reflect continuous advance field past 10 years...
Circular dichroism (CD) spectroscopy is widely used to characterize the secondary structure composition of proteins. To derive accurate and detailed structural information from CD spectra, we have developed Beta Structure Selection (BeStSel) method (PNAS, 112, E3095), which can handle spectral diversity β-structured The BeStSel webserver provides this with useful accessories community main goal analyze single or multiple protein spectra. Uniquely, on eight components including parallel...
Abstract The Database of Protein Disorder (DisProt, URL: https://disprot.org) provides manually curated annotations intrinsically disordered proteins from the literature. Here we report recent developments with DisProt (version 8), including doubling protein entries, a new disorder ontology, improvements annotation format and completely website. website includes redesigned graphical interface, better search engine, clearer API for programmatic access interface that integrates text mining...
Membraneless organelles (MOs) are dynamic liquid condensates that host a variety of specific cellular processes, such as ribosome biogenesis or RNA degradation. MOs form through liquid-liquid phase separation (LLPS), process relies on multivalent weak interactions the constituent proteins and other macromolecules. Since first discoveries certain being able to drive LLPS, it emerged general mechanism for effective organization space is exploited in all kingdoms life. While numerous...
Intrinsically disordered proteins lack a stable tertiary structure and form dynamic conformational ensembles due to their characteristic physicochemical properties amino acid composition. They are abundant in nature responsible for large variety of cellular functions. While numerous bioinformatics tools have been developed silico disorder prediction the last decades, there is need experimental methods verify state. CD spectroscopy widely used protein secondary analysis. It usable wide...
Abstract Protein aggregation and amyloid formation are linked to numerous degenerative diseases, such as Alzheimer's or Parkinson's disease. Additionally, protein plays a crucial role in various biological processes, storage of molecules cell signaling. can form wide range aggregates, from oligomers different sizes non‐specific aggregates highly ordered cross‐β structured fibrils with diverse morphologies. Circular dichroism (CD) spectroscopy is widely used technique study structures...
Circular dichroism (CD) spectroscopy is a widely used technique to characterize the secondary structure composition of proteins. We have developed Beta Structure Selection (BeStSel) method (PNAS, 112, E3095), which solves main problem protein CD spectroscopy-namely, spectral variability β-structures. The BeStSel web server utilizes this provide tools community for spectrum analysis. uniquely provides information on eight components, including parallel β-structure and antiparallel β-sheets...
Disordered plant chaperones play key roles in helping plants survive harsh conditions, and they are indispensable for seeds to remain viable. Aside from well-known thoroughly characterized globular chaperone proteins, there a number of intrinsically disordered proteins (IDPs) that can also serve as highly effective protecting agents the cells. One largest groups is group dehydrins, expressed at high levels under different abiotic stress such drought, temperature, or osmotic stress. Dehydrins...
(1) Background: Processivity is common among enzymes and mechanochemical motors that synthesize, degrade, modify or move along polymeric substrates, such as DNA, RNA, polysaccharides proteins. Processive can make multiple rounds of modification without releasing the substrate/partner, making their operation extremely effective economical. The molecular mechanism processivity rather well understood in cases when enzyme structurally confines substrate, DNA replication factor PCNA, also ATP...
Details of the functional mechanisms intrinsically disordered proteins (IDPs) in living cells is an area not frequently investigated. Here, we dissect molecular mechanism action IDP by detailed structural analyses based on in-cell nuclear magnetic resonance experiment. We show that ID stress protein (IDSP) A. thaliana Early Response to Dehydration (ERD14) capable protecting E. coli under heat stress. The overexpression ERD14 increases viability from 38.9% 73.9% following (50 °C × 15 min)....
Long non-coding RNAs (lncRNAs) are emerging as important regulators of cellular processes and extensively involved in the development different cancers; including leukemias. As one accepted methods lncRNA function is affecting chromatin structure; binding has been shown for modifiers. Histone lysine methyltransferases (HKMTs) also subject regulation demonstrated example case Polycomb Repressive Complex 2 (PRC2). Mixed Lineage Leukemia (MLL) proteins that catalyze methylation H3K4 have...
The aggregation and amyloid formation of α-synuclein is associated with Parkinson's disease other synucleinopathies. In its native, monomeric form an intrinsically disordered protein represented by highly dynamic conformational ensembles. Inhibition using small molecules, peptides, or proteins has been at the center interest in recent years. Our aim was to explore effects cross-linking on structure aggregation/amyloid properties α-synuclein. Comparative analysis available high-resolution...
Abstract Biostimulants, including phytohormones, are of high agricultural importance. Thus far, several methods have been developed for phytohormone detection/determination in microalgal cultures. Most them require expensive, state-of-the-art machinery which often has limited availability developing, countries. The main goal our study was to develop a cheap and straightforward method brassinosteroid determination We used Klebsormidium strain whose genus members reportedly content. Using...