A5077256593- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Inflammatory Biomarkers in Disease Prognosis
- RNA regulation and disease
- Viral Infections and Immunology Research
- Bacterial Genetics and Biotechnology
- Genomics and Chromatin Dynamics
- Prostate Cancer Treatment and Research
- Protein Structure and Dynamics
- Bacterial biofilms and quorum sensing
- Ubiquitin and proteasome pathways
- Plant Virus Research Studies
- Hepatitis B Virus Studies
- Antimicrobial Resistance in Staphylococcus
- Retinoids in leukemia and cellular processes
- Cancer, Lipids, and Metabolism
- Congenital heart defects research
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- Signaling Pathways in Disease
- Hepatitis C virus research
- Bacteriophages and microbial interactions
Case Western Reserve University
2016-2025
Western University of Health Sciences
2015-2025
Moderna Therapeutics (United States)
2024
University School
2015-2022
Indiana University School of Medicine
2021
Cleveland Research (United States)
2017
Case Comprehensive Cancer Center
2007-2013
University of Arkansas for Medical Sciences
2008
Pennsylvania State University
2008
Victoria University of Wellington
2005
Human melanoma cells can be reprogrammed to terminally differentiate and irreversibly lose proliferative capacity by appropriate pharmacological manipulation. Subtraction hybridization identified differentiation-associated gene-5 ( mda-5 ) as a gene induced during differentiation, cancer reversion, programmed cell death (apoptosis). This contains both caspase recruitment domain putative DExH group RNA helicase domains. Atypical motifs of MDA-5 deviate from consensus sequences but are well...
Adaptation to changes in extracellular tonicity is essential for cell survival. However, severe or chronic hyperosmotic stress induces apoptosis, which involves cytochrome c (Cyt c) release from mitochondria and subsequent apoptosome formation. Here, we show that angiogenin-induced accumulation of tRNA halves (or tiRNAs) accompanied by increased survival hyperosmotically stressed mouse embryonic fibroblasts. Treatment cells with angiogenin inhibits stress-induced formation the increases...
Abstract Liquid-liquid phase separation (LLPS) of proteins that leads to formation membrane-less organelles is critical many biochemical processes in the cell. However, dysregulated LLPS can also facilitate aberrant transitions and lead protein aggregation disease. Accordingly, there great interest identifying small molecules modulate LLPS. Here, we demonstrate 4,4’-dianilino-1,1’-binaphthyl-5,5’-disulfonic acid (bis-ANS) similar compounds are potent biphasic modulators Depending on context,...
All aspects of cellular RNA metabolism and the replication many viruses require DExH/D proteins that manipulate in a manner requires nucleoside triphosphates. Although have been shown to unwind purified duplexes, most molecules environment are complexed with proteins. It has therefore speculated may also affect RNA-protein interactions. We demonstrate DExH protein NPH-II from vaccinia virus can displace U1A an active adenosine triphosphate–dependent fashion. increases rate dissociation by...
Members of the DExH/D superfamily nucleic acid-activated nucleotide triphosphatases are essential for virtually all aspects RNA metabolism, including pre-messenger splicing, interference, translation, and nucleocytoplasmic trafficking. Physiological substrates these enzymes thought to be regions double-stranded RNA, because several proteins catalyze strand separation in vitro. These "RNA helicases" can also disrupt RNA-protein interactions, but it is unclear whether this activity coupled...
DEAD-box proteins, the largest helicase family, catalyze ATP-dependent remodeling of RNA-protein complexes and unwinding RNA duplexes. Because proteins hydrolyze ATP in an RNA-dependent fashion, energy provided by hydrolysis is commonly assumed to drive energetically unfavorable duplex unwinding. Here, we show efficient stable duplexes several presence nonhydrolyzable analog ADP-beryllium fluoride. Another analog, ADP-aluminum fluoride, does not promote The findings that from dispensable for...
RNA helicases are the largest group of enzymes in eukaryotic metabolism. The DEXD/H-box putative form helicase superfamily II, whose members defined by seven highly conserved amino acid motifs, making specific targeting selected a challenging pharmacological problem. translation initiation factor eIF4A is prototypical DEAD-box that works conjunction with eIF4B and eIF4H as subunit eIF4F to prepare mRNA template for ribosome binding, possibly unwinding secondary structure proximal 5′ m 7...
DEAD-box RNA helicases, which are involved in virtually all aspects of metabolism, generally viewed as enzymes that unwind duplexes or disrupt RNA−protein interactions an ATP-dependent manner. Here, we show vitro the protein DED1 from Saccharomyces cerevisiae promotes not only unwinding but also strand annealing, latter such a profound fashion physical limit for bimolecular association rate constant is approached. We further demonstrate establishes steady state between and enables enzyme to...
ABSTRACT Antivirulence agents inhibit the production of disease-causing virulence factors but are neither bacteriostatic nor bactericidal. against methicillin-resistant Staphylococcus aureus (MRSA) strain USA300, most widespread community-associated MRSA in United States, were discovered by virtual screening response regulator AgrA, which acts as a transcription factor for expression several prominent S. toxins and involved pathogenesis. Virtual was followed similarity searches databases...
Abstract Background Hypoxia is associated with poor prognosis in many cancers including glioblastoma (GBM). Glioma stem-like cells (GSCs) often reside hypoxic regions and serve as reservoirs for disease progression. Long non-coding RNAs (lncRNAs) have been implicated GBM. However, the lncRNAs that modulate GSC adaptations to hypoxia are poorly understood. Identification of these may provide new therapeutic strategies target GSCs under hypoxia. Methods induced by were identified RNA-seq. Lung...