A5089812187- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- Estrogen and related hormone effects
- 3D Printing in Biomedical Research
- Hypothalamic control of reproductive hormones
- Endoplasmic Reticulum Stress and Disease
- Computational Drug Discovery Methods
- Autophagy in Disease and Therapy
- Immune cells in cancer
- Cell death mechanisms and regulation
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Antioxidant Activity and Oxidative Stress
- Extracellular vesicles in disease
- Melanoma and MAPK Pathways
- Cancer Genomics and Diagnostics
- Ovarian function and disorders
- Genomics, phytochemicals, and oxidative stress
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Cancer, Stress, Anesthesia, and Immune Response
- Mitochondrial Function and Pathology
- Chromatography in Natural Products
- RNA Interference and Gene Delivery
University of Milan
2014-2024
Beckman Research Institute
2020
City of Hope
2020
City Of Hope National Medical Center
2018
Abstract Prostate cancer (PC) is one of the leading causes death in males. Available treatments often lead to appearance chemoresistant foci and metastases, with mechanisms still partially unknown. Within tumour mass, autophagy may promote cell survival by enhancing cells tolerability different stresses, like hypoxia, starvation or those triggered chemotherapic agents. Because its connection apoptotic pathways, has been differentially implicated, either as prodeath prosurvival factor, more...
Abstract Objectives Prostate cancer, after the phase of androgen dependence, may progress to castration‐resistant prostate cancer (CRPC) stage, with resistance standard therapies. Vitamin E‐derived tocotrienols (TTs) possess a significant antitumour activity. Here, we evaluated anti‐cancer properties δ‐TT in CRPC cells (PC3 and DU145) related mechanisms action. Materials methods MTT, Trypan blue colony formation assays were used assess cell viability/cell death/cytotoxicity. Western blot,...
Abstract Melanoma is an aggressive tumor with still poor therapy outcomes. δ-tocotrienol (δ-TT) a vitamin E derivative displaying potent anti-cancer properties. Previously, we demonstrated that δ-TT triggers apoptosis in human melanoma cells. Here, investigated whether it might also activate paraptosis, non-canonical programmed cell death. In accordance the main paraptotic features, was shown to promote cytoplasmic vacuolization, associated endoplasmic reticulum/mitochondrial dilation and...
In vitro diffusive models are an important tool to screen the penetration ability of active ingredients in various formulations. A reliable assessment skin enhancing properties, mechanism action carrier systems, and estimation a bioavailability essential for transdermal delivery. Given importance testing kinetics different compounds across barrier, several have been developedThe aim this study was compare Franz Diffusion Cell (FDC) with novel fluid-dynamic platform (MIVO) by evaluating...
Abstract Malignant melanoma is the leading cause of death from skin cancer. Drug toxicity and resistance represent a serious challange for treatments. Evidence demonstrates that natural compounds may play crucial role in cancer prevention, growth progression. Vitamin E tocotrienols (TT) were shown to possess antitumor activity. Here, we analyzed effects δ-TT on cell involvement endoplasmic reticulum (ER) stress this The experiments performed human lines, BLM A375. exerted significant...
Extracellular vesicles (EVs) are naturally occurring cargo delivery that have recently received considerable attention for their roles in intercellular communication many physiological and pathological processes, including tumourigenesis. EVs generated by different tissues demonstrated specific homing: particular, cancer-derived showed a selective tropism the tumor tissue from which originated. For this property, been proposed as drug tools anti-cancer therapies, although limited knowledge...
Three-dimensional (3D) cell cultures allow the mimic of functions living tissues and provide key information encoded in tissue architecture. Considered pivotal role epithelial-to-mesenchymal transition (EMT) carcinoma progression, including prostate cancer (PCa), we aimed at investigating effect 3D arrangement on expression some markers EMT cultured human (PCa) cells, to better understand PCa behavior. PC3 DU145 cells were RPMI culture medium either 2D-monolayers or 3D-spheroids. The main...
The success of immunotherapeutic approaches strictly depends on the immune cells interaction with cancer cells. While conventional in vitro cell cultures under-represent complexity and dynamic crosstalk tumor microenvironment, animal models do not allow deciphering anti-tumor activity human system. Therefore, development reliable predictive preclinical has become crucial for screening immune-therapeutic approaches. We here present an organ-on-chip organ chips (OOC)-based approach...
Abstract The prognosis of metastatic melanoma is very poor, due to the development drug resistance. Cancer stem cells (CSCs) may play a crucial role in this mechanism, contributing disease relapse. We first characterized CSCs cell lines. observed that A375 (but not BLM) are able form melanospheres and show traits: expression pluripotency markers SOX2 KLF4, higher invasiveness tumor formation capability vivo with respect parental adherent cells. also showed subpopulation autofluorescent...
Abstract Objectives Among gynaecologic malignancies, ovarian cancer (OC) represents the leading cause of death for women worldwide. Current OC treatment involves cytoreductive surgery followed by platinum‐based chemotherapy, which is associated with severe side effects and development drug resistance. Therefore, new therapeutic strategies are urgently needed. Herein, we evaluated anti‐tumour Vitamin E‐derived δ‐tocotrienol (δ‐TT) in two human cell lines, IGROV‐1 SKOV‐3 cells. Materials...
It is now well-established that cancer stem cells (CSCs) can support melanoma progression by reshaping the tumor immune microenvironment. However, molecular mechanisms underlying crosstalk between SCs and cancer-associated neutrophils have not been elucidated yet.The aim of present study was to unravel role in neutrophil polarization. HL60 neutrophil-like (dHL60) were treated with conditioned medium from A375 (CSC-CM), their phenotype investigated.We demonstrated CSC-CM could specifically...
Background Cutaneous melanoma is an aggressive malignancy; its incidence increasing worldwide and prognosis remains poor. Clinical observations indicate that estrogen receptor β (ERβ) expressed in tissues expression decreases with tumor progression, suggesting suppressive function. These experiments were performed to investigate the effects of ERβ activation on cell growth. Methods Results Protein was analyzed by Western blot immunofluorescence assays. Cell proliferation assessed counting...