Vulvovaginal candidiasis (VVC) is an infection of the genital mucosa caused by different species genus Candida. Considering lack data on this topic in south Brazil, study aimed to assess prevalence Candida spp. cervical-vaginal patients treated at a university hospital southern Rio Grande do Sul, as well etiology and susceptibility isolates against fluconazole, itraconazole, miconazole nystatin. Samples were collected gynecology clinic Federal Hospital University Grande, identified using...

Abstract The presence of airborne fungi in Intensive Care Unit (ICUs) is associated with increased nosocomial infections. aim this study was the isolation and identification presented an ICU from University Hospital Pelotas – RS, attempt to know place’s environmental microbiota. 40 Petri plates Sabouraud Dextrose Agar were exposed environment ICU, where samples collected strategic places during morning afternoon periods for ten days. Seven genera identified: Penicillium spp. (15.18%), genus...

Abstract Mastitis is an inflammatory process of the udder tissue caused mainly by bacteria Staphylococcus aureus. The indiscriminate use antibiotics fosters conditions that favor selection resistant microorganisms, suppressing at same time susceptible forms, causing a serious problem in dairy cattle. Given importance performing antibiogram to select most adequate antimicrobial therapy, aim this study was identify isolated from cow’s milk with mastitis, farms situated city Pelotas, Rio Grande...

Sporothrix brasiliensis is the main agent of feline and zoonotic sporotrichosis. Given scarcity options for sporotrichosis treatment promising activity diphenyl diselenide (PhSe)2 against a diversity fungal pathogens, this study aimed to evaluate in vitro susceptibility alone combination with itraconazole (ITC). Forty clinical isolates S. were subjected broth microdilution method their concentrations ranging between 0.25 128 μg/ml, ITC (0.0313-16 μg/ml) following protocol Clinical Laboratory...

Summary Paracoccidioidomycosis ( PCM ) is a systemic mycosis that occurs in several Latin American countries, especially Brazil. It caused by the thermo‐dimorphic fungus Paracoccidioides spp. Serological studies to detect animal infection represent an excellent strategy for data on agent's ecology. Although state of Rio Grande do Sul RS endemic area humans, there scarce information available ecology agent region. This study aimed investigate lutzii animals living , A total 85 wild mammals,...

The identification of the fungal species belonging to healthy microflora in animals is a precondition for recognition pathological processes causing them. aim this study was investigate presence potentially pathogenic fungi feces wild birds collected Screening Centers. Samples were from 50 cages with different birds. samples processed according modified method STAIB and plates incubated at 32 °C up ten days daily observation detection growth. isolation following observed: Malassezia...

The fast anthelmintic resistance development has shown a limited efficiency in the control of animal's endoparasitosis and promoted research using alternative methods. use chemicals animal treatment, association with nematophagous fungi used for biological control, is strategy that proven to be effective reducing nematode population density farm animals. This study aims verify vitro susceptibility Arthrobotrys oligospora, Duddingtonia flagrans Paecilomyces lilacinus against antiparasitic...

Abstract The aim of this study was to evaluate the frequency Candida species between a non-hospitalized and hospitalized population. For purpose, samples saliva were sampled through sterile swabs, moistened in peptone water rubbed oral cavity 140 individuals, from which, 70 patients Medical Clinic Teaching Hospital other subjects. All plated Sabouraud Dextrose agar added with Chloramphenicol incubated at 36 °C for 48 hours. morphology identification performed macroscopic microscopic...

Yeast infections have acquired great importance due to increasing frequency in immunocompromised patients or undergoing invasive diagnostic and therapeutic techniques, also because of its high morbidity mortality. At the same time, it has been seen an increase emergence new pathogenic species difficult diagnose treat. The aim this study was determine vitro susceptibility 89 yeasts from different sources against antifungals amphotericin B, voriconazole, fluconazole flucytosine, using VITEK® 2...

Even today, an effective diagnostic test for aspergillosis in penguins is unknown, being the gold standard post-mortem examinations. The fungal antigen galactomannan (GM) has been used as a biomarker of disease humans and detected by Platelia Aspergillus EIA (BioRad)®, commercial kit based on sandwich ELISA technique. It standardized use neutropenic patients, however studies have demonstrated its usefulness also possible birds. aim our study was to evaluate effectiveness EIA® (BioRad-US)...

Abstract The increase in zoonotic sporotrichosis cases has become a public health problem hyperendemic areas for the feline disease. Domestic cats involvement this transmission and continuous contact of these animals with soil suggest that environment may be an essential source and/or reservoir Sporothrix spp. Thus, current study aimed to evaluate presence places where infected Southern Brazil is present. In total, 101 samples were collected from residence sporotrichosis, regions around...

The use of biological agents has been intensified in recent years against eggs and larvae gastrointestinal nematodes as an alternative control method pasture plant health management, with the concomitant antiparasitic drugs still occurring. aim this study was to test vitro activity following drugs: Ivermectin albendazole nematophagous fungi: Paecilomyces fumosoroseus, lilacinus variotii. agar diffusion performed using initial concentration 0.0016g/mL each drug, after solidification culture...

Malassezia pachydermatis is a commensal yeast of the skin and external ear canal dogs cats that may become an opportunistic pathogen under some conditions. The aim this paper was to research diseases which predispose development M. in with otitis or dermatopathies. samples were collected by sterile swabs carpet technique. Samples obtained from cutaneous tegument seeded Sabouraud dextrose agar added chloramphenicol cycloheximide. Following, they incubated at 32ºC for seven days...

Infeccoes hospitalares estao relacionadas com altos indices de morbidade e mortalidade em pacientes internados. Objetivou-se identificar as leveduras presentes no ambiente UTI hospital do municipio Pelotas, RS. A coleta ocorreu por friccao swabs sedimentacao placas. Foram identificadas Candida parapsilosis (27,6%), C. guillermondii (10,3%), albicans (6,9%), famata sp. (3,4%), Rhodotorulla spp. (20,7%), Cryptococcus laurentii Saccharomyces cerevisae (10,3%) Stephanoascus ciferri sendo...

INTRODUCTION: Previous studies that detected Paracoccidioides spp. DNA in soil taken from rural areas have shown this to be a valuable tool for ecological and epidemiological studies. This study reports the detection of samples an urban area southern Brazil. METHODS: Sixteen were submitted nested-PCR amplicons representative number positive sequenced. RESULTS: was found 44% samples. Four sequenced, showing 100% homology with P. brasiliensis. CONCLUSIONS: The Brazilian population is commonly...

Hospital environments can have pathogens and opportunistic pathogens, important for immunocompromising individuals. The process of cleaning disinfection constitutes an measure the control these diseases. This study evaluated sensitivity yeasts isolated from surfaces nosocomial veterinary environment to four disinfectants/antiseptics used in routine disinfection. test broth microdilution was carried 24 isolates against 4% sodium hypochlorite, 2% benzalkonium chloride, 6.6%...

Several factors can cause false-positive results in the galactomannan (GM) test; however, others remain unknown. Presently, impact of airborne contamination by Aspergillus conidia during enzyme-linked immunosorbent assay (ELISA) remains uninvestigated.We studied 12 A. fumigatus isolates. Fungal were serially diluted and tested for GM detection using Platelia® enzyme immunoassay (EIA).The concentration required an EIA-positive result was 4.8 × 103 (median).This is first study to evaluate...