A5100336803- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Tissue Engineering and Regenerative Medicine
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Machine Learning in Bioinformatics
- Pesticide Residue Analysis and Safety
- Total Knee Arthroplasty Outcomes
- Microbial Metabolic Engineering and Bioproduction
- 3D Printing in Biomedical Research
- Plant Gene Expression Analysis
- Mycobacterium research and diagnosis
- Electrospun Nanofibers in Biomedical Applications
- Spectroscopy and Laser Applications
- Neuroscience and Neuropharmacology Research
- Natural product bioactivities and synthesis
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Chemical Sensor Technologies
- Functional Brain Connectivity Studies
- Neural dynamics and brain function
Shandong University of Traditional Chinese Medicine
2024-2025
Chinese Academy of Sciences
2012-2024
Southwest Forestry University
2015-2024
Huazhong University of Science and Technology
2024
Ministry of Ecology and Environment
2024
Qingdao University
2009-2024
Affiliated Hospital of Qingdao University
2009-2024
Central South University
2003-2024
University of Southern California
2011-2024
Nankai University
2024
The lipid droplet (LD) is a universal organelle governing the storage and turnover of neutral lipids. Mounting evidence indicates that elevated intramuscular triglyceride (IMTG) in skeletal muscle LDs closely associated with insulin resistance Type 2 Diabetes Mellitus (T2DM). Therefore, identification LD proteome will provide some clues to dissect mechanism connecting IMTG T2DM. In present work, we identified 324 LD-associated proteins mouse through mass spectrometry analysis. Besides...
Mechanistic arguments relative to matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) address observations that predominately singly charged ions are detected. However, recently a matrix assisted ablation method, laserspray ionization (LSI), was introduced can use the same sample preparation and as MALDI, but produce highly from proteins. In generated neutral molecules by photon energy provided matrix, while in LSI produced inside heated inlet tube linking atmospheric...
The postsynaptic site of neurons is composed more than 1500 proteins arranged in protein-protein interaction complexes, the composition which modulated by protein phosphorylation through actions complex signaling networks. Components these networks function as key regulators synaptic plasticity, particular hippocampal long-term potentiation (LTP). density (PSD) a multicomponent structure that includes receptors, enzymes, scaffold proteins, and structural proteins. We triggered LTP mouse...
Shotgun proteomics data analysis usually relies on database search. However, commonly used protein sequence databases do not contain information variants and thus prevent variant peptides proteins from been identified. Including known coding variations into could help alleviate this problem. Based our recently published human Cancer Proteome Variation Database, we have created a that comprehensively annotates thousands of cancer-related collected in the Database as well noncancer-specific...
Irritable bowel syndrome (IBS) is at high risk of co-morbid depression and anxiety, which reduces patients' quality life increases the burden health care costs. However, pathophysiological mechanisms responsible for IBS still remain unknown. This study investigated effects resveratrol on stress-related depression, intestinal visceral dysfunction in rat model IBS. Rats received chronic acute combining stress (CACS) procedures 22 days exhibited depression/anxiety-like behavior,...
Effective therapeutic strategies for treating chronic spinal cord injury (SCI) are currently unavailable. Scar tissue in the lesion area is a main inhibitory factor axonal regeneration and repair of SCI. In this study, scar was surgically resected from adult rats with 12 week SCI then collagen scaffold (NeuroRegen Scaffold; NRS) human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were implanted into cavity to The results demonstrated that locomotor function not affected by...
Identification and annotation of mutated genes or proteins involved in oncogenesis tumor progression are crucial for both cancer biology clinical applications. We have developed a human Cancer Proteome Variation Database (CanProVar) by integrating information on protein sequence variations from various public resources, with focus cancer-related (crVAR). also built user-friendly interface querying the database. The current version CanProVar comprises 8,570 crVARs 2,921 derived existing...
An efficient and sensitive chiral analytical method was established for the determination of propiconazole stereoisomers by supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS). Stereoisomeric separation performed on a Chiralpak AD-3 column with CO2/ethanol (93:7) as mobile phase. The four were well separated in 4.7 min resolutions above 2.0. specificity, linearity, matrix effects, accuracy, precision, stability developed evaluated. stereoselective dissipation wheat straw,...
The differentiation of pericytes into myofibroblasts causes microvascular degeneration, ECM (extracellular matrix) accumulation, and tissue stiffening, characteristics fibrotic diseases. It is unclear how pericyte-myofibroblast regulated in the environment. Our previous study established a novel 2-dimensional platform for coculturing endothelial cells (ECs) derived from same tissue. This investigated stiffness ECs, pericytes, their interactions.Primary microvessels were cultured TGM2D medium...
Mitochondria play essential roles in cell physiological processes including energy production, metabolism, ion homeostasis, growth, aging and apoptosis. Proteomic strategies have been applied to the study of mitochondria since 1998; these studies yielded decisive information about diverse functions organelle. As an ideal model biological system, nematode Caenorhabditis elegans has widely used several diseases, such as metabolic diseases cancer. However, mitochondrial proteome C. remains...
The stress protein heme oxygenase-1 (HO-1) is upregulated and co-localizes to pathological features, including tauopathies in the brains of individuals with Alzheimer's disease. However, relationship between HO-1 disease remains u
GTPase-activating proteins (GAPs) and guanine exchange factors (GEFs) play essential roles in regulating the activity of small GTPases. Several GAPs GEFs have been shown to be present at postsynaptic density (PSD) within excitatory glutamatergic neurons regulate glutamate receptors. However, it is not known how synaptic GAP GEF are organized PSD signaling machinery, if they overlapping interaction networks, or associate with implicated contributing psychiatric disease. Here, we determine...