A5101688357- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Light effects on plants
- Mitochondrial Function and Pathology
- Plant Physiology and Cultivation Studies
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Biotin and Related Studies
- Enzyme Production and Characterization
- Legume Nitrogen Fixing Symbiosis
- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Microbial Metabolites in Food Biotechnology
- Insect Resistance and Genetics
- Botanical Research and Applications
- Potato Plant Research
- Soil Carbon and Nitrogen Dynamics
- Genetically Modified Organisms Research
- Plant responses to elevated CO2
- Horticultural and Viticultural Research
- Lipid metabolism and biosynthesis
- CRISPR and Genetic Engineering
- Bioenergy crop production and management
Institute of Botany
2016-2022
Chinese Academy of Sciences
2014-2022
University of Chinese Academy of Sciences
2018-2022
Czech Academy of Sciences, Institute of Botany
2014
Michigan State University
2000-2007
Massachusetts General Hospital
2003
Field Crops Research Institute
2003
University of Siena
2003
Agricultural Research Organization
1997-1999
Stellenbosch University
1999
The Populus shoot undergoes primary growth (longitudinal growth) followed by secondary (radial growth), which produces biomass that is an important source of energy worldwide. We adopted joint PacBio Iso-Seq and RNA-seq analysis to identify differentially expressed transcripts along a developmental gradient from the apex fifth internode Nanlin895. obtained 87 150 full-length transcripts, including 2081 new isoforms 62 058 alternatively spliced isoforms, most were produced intron retention,...
Abstract The acyclic polyol sorbitol is a primary photosynthetic product and the principal transport substance in many economically important members of family Rosaceace (e.g. almond [Prunus dulcis (P. Mill.) D.A. Webber], apple [Malus pumila P. Mill.], cherry spp.], peach persicaL. Batsch], pear [Pyrus communis]). To understand key steps long-distance particularly partitioning accumulation sink tissues, we have cloned two transporter genes (PcSOT1 andPcSOT2) from sour (Prunus cerasus) fruit...
Demand for the commercial use of genetically modified (GM) crops has been increasing in light projected growth world population to nine billion by 2050. A prerequisite paramount importance regulatory submissions is rigorous safety assessment GM crops. One components molecular characterization at DNA level which helps determine copy number, integrity and stability a transgene; characterize integration site within host genome; confirm absence vector DNA. Historically, carried out using...
The cucurbits translocate the galactosyl-sucrose oligosaccharides raffinose and stachyose, therefore, alpha-galactosidase (alpha-D-galactoside galactohydrolase, EC 3.2.1.22) is expected to function as initial enzyme of photoassimilate catabolism. However, previously described alkaline specific for tetrasaccharide leaving catabolism in these tissues an enigma. In this paper we report partial purification characterization three alpha-galactosidases, including a novel (form I) from melon...
Summary Raffinose and stachyose are ubiquitous galactosyl‐sucrose oligosaccharides in the plant kingdom which play major roles, second only to sucrose, photoassimilate translocation seed carbohydrate storage. These sugars initially metabolised by α‐galactosidases (α‐gal). We report cloning functional expression of first genes, CmAGA1 CmAGA2 , encoding for α‐gals with alkaline pH optima from melon fruit ( Cucumis melo L.), a raffinose translocating species. The α‐gal genes show very high...
In apple ( Malus ×domestica Borkh.), where sorbitol is a primary photosynthetic product that translocated throughout the plant, accumulation of in sink cells appears to require an active carrier-mediated membrane transport step. Recent progress isolation and characterization genes for transporters sour cherry Prunus cerasus L.) mannitol celery Apium graveolens suggested similar may be present tissues. A defect these could also explain occurrence fruit disorder watercore, characterized by...
In celery, mannitol is a primary photosynthetic product that associated with celery's exceptional salt tolerance. Arabidopsis plants transformed mannose-6-phosphate reductase (M6PR) gene produce and grow normally in the absence of stress. Daily analysis increase growth (fresh dry weight, leaf number, area per plant specific weight) over 12-day period showed less effect (100 mm NaCl) on M2 transformant than wild type (WT). Following treatment WT, M5 100 or 200 NaCl total shoot fresh were...
In C4 plants, pyruvate orthophosphate dikinase (PPDK) activity is tightly dark/light regulated by reversible phosphorylation of an active-site threonine (Thr) residue; this process catalyzed PPDK regulatory protein (PDRP). Phosphorylation and dephosphorylation lead to its inactivation activation, respectively. Here, we show that light intensity rather than the light/dark transition regulates modulating at Thr-527 (previously termed Thr-456) in maize (Zea mays). The amount (unphosphorylated)...
In sink tissues of cucurbits, including sweet melon fruits, the galactosyl‐sucrose oligosaccharides, stachyose and raffinose, together with sucrose, are major translocated carbohydrates. present study we investigated carbohydrate metabolism young ( Cucumis melo L. cv. C‐8) fruit during period initial set development, from 3 days prior to anthesis until 20 after (DAA), onset sucrose accumulation. The enzymes assayed could be classified into two categories according developmental patterns. Two...
Abstract Mannitol, a sugar alcohol, is major primary photosynthetic product in celery (Apium graveolens L. cv Giant Pascal). We report here on purification, characterization, and cDNA cloning of cytosolic non-reversible glyceraldehyde-3-P dehydrogenase (nr-G3PDH, EC1.2.1.9), the apparent key contributor NADPH required for mannitol biosynthesis leaves. As determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, purified nr-G3PDH showed molecular mass 53 kD. A 1,734-bp...
Abstract De-etiolation consists of a series developmental and physiological changes that plant undergoes in response to light. During this process light, an important environmental signal, triggers the inhibition mesocotyl elongation production photosynthetically active chloroplasts, etiolated leaves transition from “sink” stage “source” stage. has been extensively studied maize (Zea mays L.). However, little is known about how regulated. In study, we described quantitative proteomic...
A preliminary understanding of developmental processes among divergent species is essential to evaluate the applicability information from model plants agricultural importance. In tomato ( Lycopersicon esculentum Mill.), where molecular biology associated with fruit ripening has been studied most extensively, tissue softening due at least in part activity proteins called expansins, concert enzymatic activities that modify pectin and xyloglucan components cell wall. We evaluated potential for...
SUMMARY As sessile organisms, plants need to respond rapid changes in numerous environmental factors, mainly diurnal of light, temperature, and humidity. Maize is the world's most grown crop, as a C4 plant it exhibits high photosynthesis capacity, reaching highest rate net at midday; that is, there no “midday depression.” Revealing physiological responses underlying mechanisms will be great significance for guiding maize improvement efforts. In this study, we collected leaf samples analyzed...
The influence of variation in the concentration dissolved inorganic carbon (DIC) form CO 2 and HCO 3 − root media on C N metabolism Lycopersicon esculentum cv. F144 was investigated under both saline non‐saline conditions. Tomato seedlings were grown hydroponic culture (pH 6.5) with or without NaCl, solution aerated either ambient (360 μmol mol −1 ) ‐enriched air (5000 ). Nitrate uptake tissue NO concentrations increased slightly by elevated rhizosphere DIC control salinity‐treated plants....
We investigated the influence of an increased inorganic carbon supply in root medium on NO − 3 uptake and assimilation seedlings Lycopersicon esculentum (L.) Mill. cv. F144. The were pre‐grown for 4 to 7 days with 0 or 100 m M NaCl hydroponic culture using 0.2 (group A) NH + B) as nitrogen source. nutrient solution group A plants was aerated air containing 800 μumol mol −1 CO 2 . Nitrate rate leaf malate contents these determined. B subdivided into two sets. Plants one set transferred either...
Abstract De-etiolation consists of a series developmental and physiological changes that plant undergoes in response to light. During this process light, an important environmental signal, triggers the inhibition mesocotyl elongation production photosynthetically active chloroplasts, etiolated leaves transition from “sink” stage “source” stage. has been extensively studied maize ( Zea mays L ). However, little is known about how regulated. In study, we describe quantitative proteomic...
P. Bagnaresi, Pupillo, M. Bartels, W. Schmidt, L. Bláha, J. Janáček, Opatrná, Cerezo, Garcia-Agustin, E. Primo-Millo, Cieply, T. Oracka, I. Ciereszko, A. Gniazdowska, Rychter, R. Costa, C. Ricardo, D. Cramer, Schierholt, Y. Z. Wang, S. H. Lips, G. Farhytdinov, Kudoyarova, F. Fodor, Cseh, Dao Thi Phu Ong, Gy. Záray, Fox, B. Loughman, N. Mccallan, Quiquampoix, Ratcliffe, Gao, Gunse, Ch. Poschenrieder, Barcelo, V. Hadži-Tašković Šukalović, Vuletić, Holub, Vizárová, Ješko, K. Dekánková, Š....