A5046274447- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Microbial Community Ecology and Physiology
- Virus-based gene therapy research
- Animal Virus Infections Studies
- RNA modifications and cancer
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- Respiratory viral infections research
- Parasitic Infections and Diagnostics
- Aeolian processes and effects
- Aquaculture disease management and microbiota
- Archaeological and Historical Studies
- Enzyme Structure and Function
- Toxoplasma gondii Research Studies
- Bacterial Genetics and Biotechnology
- Infant Nutrition and Health
- Insect symbiosis and bacterial influences
- Lichen and fungal ecology
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- Administrative Law and Governance
University of Copenhagen
2014-2025
University of Pretoria
2019-2023
Weatherford College
2023
National Institutes of Health
2023
National Center for Biotechnology Information
2023
Universidad Nacional Autónoma de México
2013
The production of biochemicals requires the use microbial strains with efficient substrate conversion and excellent environmental robustness, such as Weizmannia coagulans species. So far, genomes 47 have been sequenced. Herein, we report a comparative genomic analysis nine on full repertoire Carbohydrate-Active enZymes (CAZymes), secretion systems, resistance mechanisms to challenges. Moreover, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) immune system along...
Abstract In silico identification of viral anti-CRISPR proteins (Acrs) has relied largely on the guilt-by-association method using known Acrs or associated (Acas) as bait. However, low number and limited spread characterized archaeal Aca hinders our ability to identify guilt-by-association. Here, based observation that few are transcribed immediately post infection, we hypothesize these genes, many other unidentified anti-defense genes (ADG), under control conserved regulatory sequences...
Archaeal antiviral defense systems remain poorly characterized despite recent advances in understanding prokaryotic immunity. Here, we analyze 7,747 archaeal genomes, the largest and most diverse dataset to date, revealing a striking disparity system prevalence diversity compared Bacteria. Nearly one-third of genomes lack known beyond CRISPR-Cas restriction-modification (in contrast only 2.2% bacterial genomes), 31% contain systems, far below previous estimates. While many appear restricted...
<title>Abstract</title> Archaeal antiviral defense systems remain poorly characterized despite recent advances in understanding prokaryotic immunity. Here, we analyze 7,747 archaeal genomes, the largest and most diverse dataset to date, revealing a striking disparity system prevalence diversity compared Bacteria. Nearly one-third of genomes lack known beyond CRISPR-Cas restriction-modification (in contrast only 2.2% bacterial genomes), 31% contain systems, far below previous estimates. While...
We provide here, for the first time, insights into initial infection stages of a large spindle-shaped archaeal virus and explore following life cycle events. Our observations suggest that Sulfolobus monocaudavirus 1 (SMV1) exhibits high adsorption rate virions adsorb to host cells via three distinct attachment modes: nosecone association, body body/tail association. In association mode, entire virion, including tail(s), aligns cell surface main is greatly flattened, suggesting possible...
ABSTRACT Sulfolobus mutants resistant to archaeal lytic virus islandicus rod-shaped 2 (SIRV2) were isolated, and mutations identified in two gene clusters, cluster sso3138 sso3141 sso2386 sso2387 , encoding cell surface type IV secretion proteins, respectively. The involvement of the resistance was confirmed by genetic complementation. Blocking entry into demonstrated lack early transcription, strongly supporting idea a role proteins SIRV2 entry.
Viral factories are compartmentalized centers for viral replication and assembly in infected eukaryotic cells. Here, we report the formation of a focus by prototypical archaeal Sulfolobus islandicus rod-shaped virus 2 (SIRV2) model archaeon This belongs to family Rudiviridae, carrying linear double-stranded DNA (dsDNA) genomes, which very common geothermal environments. We demonstrate that SIRV2 synthesis is confined near periphery Moreover, cellular proteins recruited to, concentrated in,...
Linear dsDNA replicons with hairpin ends are found in the three domains of life, mainly associated plasmids and viruses including poxviruses, some phages archaeal rudiviruses. However, their replication mechanism is not clearly understood. In this study, we find that rudivirus SIRV2 undergoes multiple consecutive reinitiation events at genomic termini. Using a strand-displacement strategy, from one parental template yield highly branched intermediates corresponding to about 30 genome units...
Abstract Bacteriophages and bacteria are engaged in a constant arms race, continually evolving new molecular tools to survive one another. To protect their genomic DNA from restriction enzymes, the most common bacterial defence systems, double-stranded phages have evolved complex modifications that affect all four bases. This study focuses on at position 7 of guanines. Eight derivatives 7-deazaguanines were identified, including previously unknown ones:...
ABSTRACT Viral members of the protein family DUF1874 have been reported to act as anti-CRISPR (acr) proteins that degrade cyclic tetra-adenylate (cA 4 ), a nucleotide second messenger produced after activation several type III CRISPR-Cas systems in bacteria and archaea. Specifically, SIRV1 gp29 inhibits III-A III-B CRISPR plasmid-born assays heterologous systems. In this work, we investigate function its close homolog SIRV2 gp37 native biological context, i.e. cultures infected by SIRV2. was...
Abstract Cell cycle regulation is crucial for all living organisms and often targeted by viruses to facilitate their own propagation, yet cell progression control largely underexplored in archaea. In this work, we reveal a regulator (aCcr1) carrying ribbon-helix-helix (RHH) domain ubiquitous the Thermoproteota of order Sulfolobales viruses. Overexpression several aCcr1 members including gp21 rudivirus SIRV2 its host homolog SiL_0190 Saccharolobus islandicus LAL14/1 results impairment...
ABSTRACT Salt pans or playas, which are saline-rich springs surrounded by halite evaporates in arid environments, have played an essential role landscape erosion during the formation of Namib Desert and numerous its central region. In this study, we used shotgun metagenomics to investigate phylogenetic functional capacities microbial communities from two salt (namely, Eisefeld Hosabes) located Desert, Southwest Africa. We studied source sink sediment mat saline streams, as well those halites...
A hallmark of group/species rotavirus (RVA) replication in MA-104 cells is the logarithmic increase viral mRNAs that occurs four-12 h post-infection. Viral protein synthesis typically lags closely behind mRNA but continues after levels plateau. However, RVA non-structural 1 (NSP1) present at very low throughout despite showing robust synthesis. NSP1 has contrasting properties being susceptible to proteasomal degradation, stabilised against degradation by proteins and/or mRNAs. We aimed...
<title>Abstract</title> In silico identification of viral anti-CRISPR proteins (Acrs) has relied largely on the guilt-by-association method with known Acrs or associated (Acas) used as bait. However, low number and limited spread characterized archaeal Acas hinders our ability to identify using guilt-by-association. Here, based an observation that few Aca are transcribed immediately post infection, we hypothesized these genes, many other unidentified anti-defense under control conserved...
Abstract Salt pans or playas, which are saline-rich springs surrounded by halite evaporates in arid environments, have played an essential role landscape erosion during the formation of Namib Desert and numerous its central region. In this study, we used shotgun metagenomics to investigate phylogenetic functional capacities microbial communities from two salt (namely, Eisefeld Hosabes) located Central Desert, Southwest Africa. We studied source sink sediment mat saline streams, as well those...
ABSTRACT Cell cycle regulation is crucial for all living organisms and often targeted by viruses to facilitate their own propagation, yet cell progression control largely underexplored in archaea. In this work, we reveal a regulator (aCcr1) carrying ribbon-helix-helix (RHH) domain ubiquitous the crenarchaeal order Sulfolobales viruses. Overexpression of several aCcr1 members including gp21 rudivirus SIRV2 its host homolog SiL_0190 S. islandicus LAL14/1 results impairment division, evidenced...
ABSTRACT Bacteriophages and bacteria are engaged in a constant arms race, continually evolving new molecular tools to survive one another. To protect their genomic DNA from restriction enzymes, the most common bacterial defence systems, double-stranded phages have evolved complex modifications that affect all four bases. This study focuses on at position 7 of guanines. Eight derivatives 7-deazaguanines were identified, including previously unknown ones:...