A5038354519- Genetic Neurodegenerative Diseases
- Dental Implant Techniques and Outcomes
- Anatomy and Medical Technology
- Animal Behavior and Welfare Studies
- RNA Research and Splicing
- Effects of Environmental Stressors on Livestock
- RNA modifications and cancer
- Neuroscience and Neural Engineering
- Neurological diseases and metabolism
- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Agriculture and Farm Safety
- Genomics and Chromatin Dynamics
- RNA regulation and disease
- Dental materials and restorations
- CRISPR and Genetic Engineering
- Human-Animal Interaction Studies
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
University of Wisconsin–River Falls
2019-2024
Case Western Reserve University
2018-2022
University School
2018-2022
Washington University in St. Louis
2018-2020
Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic induced pluripotent stem cells, independent strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides powerful platform the...
Dairy cows are regularly handled when moved to the milking parlor and during other routine procedures. Low-stress handling methods important in avoiding negative welfare states for dairy cattle. Tail twisting is used by some handlers prompt cattle movement. However, inappropriately with excessive force, tail can lead a broken tail. The aim of this cross-sectional study was determine cow-level factors that may be associated prevalence tails A subset 229 Holstein (68 primiparous 161...
The objective of this study was to contrast the soft tissue thickness, cranial total cross-sectional brain area, and bolt-brain contact from common frontal application captive bolt euthanasia with alternative location behind ear in cadaver swine heads. Twenty-three heads pigs that were approximately 136 kg 6 mo age collected a regional slaughter establishment following CO2 stunning assigned either FRONTAL (n = 11) or CAUDAL TO PINNA 12) bolt. thickness different (P < 0.0001) between 2...
Abstract The objective of this project was to determine the impact cooling on soft tissue thickness, cranial and cross-sectional brain area cadaver heads from market pigs. Documenting effect dimensions swine is valuable important for future investigations physical stunning euthanasia methods that use heads. Scalded dehaired with intact jowls were sourced pigs stunned CO2 gas. After transport data collection location, a penetrating captive bolt (PCB) shot (Jarvis Model PAS—Type P 0.25R...
Many indoor-housed cows isolate at calving when given the opportunity, and behaviors vary by blind pen design. The objectives of this study were to determine if preferred in a visibly separated (blind) or an open area group maternity pen, there was preference for degree seclusion provided (50% vs. 100% coverage). Two blinds amount visibility through created using firehoses secured from top metal frame that lined entire front (100%) with every other hose rolled up (50%). Holstein heifers (n =...
Mutations in proteolipid protein 1 ( PLP1 ) result failure of myelination and severe neurological dysfunction the X-linked pediatric leukodystrophy Pelizaeus-Merzbacher disease (PMD). The majority variants, including supernumerary copies various point mutations, lead to early mortality. However, -null patients mice display comparatively mild phenotypes, suggesting that reduction aberrant expression might provide a therapeutic strategy across PMD genotypes. Here we show, CRISPR-Cas9 mediated...
SUMMARY Pluripotent stem cells (PSCs) provide an unlimited source for generating somatic cell types. However, fully mature constitutes a bottleneck realizing their full potential in research and medicine. Here, we report transcriptional mechanism that governs the timing of cellular maturation post-mitotic oligodendrocytes. During differentiation PSCs to oligodendrocytes, transcription factor SOX6 redistributes from nearly all super enhancers proliferating oligodendrocyte progenitor cluster...
Abstract The objective of this study was to contrast the soft tissue thickness, cranial total cross-sectional brain area and bolt–brain contact from common frontal application captive bolt euthanasia with alternative location behind ear in cadaver swine heads. Twenty-three heads pigs that were approximately 136 kg 6 mo age collected a slaughter establishment following CO2 stunning assigned either FRONTAL (n = 11) or CAUDAL TO PINNA 12) bolt. thickness different (P &lt; 0.0001) between 2...
Abstract The objective of this project was to determine the impact cooling on soft tissue thickness, cranial and cross-sectional brain area cadaver heads from market pigs. Documenting effect dimensions swine is valuable important for future investigations physical stunning euthanasia methods that use heads. Cadaver were sourced pigs stunned with CO2. After transport data collection location, a captive bolt shot applied in frontal position. Following application, each head (n=36) underwent an...