A5083403093- Plant Molecular Biology Research
- Plant Reproductive Biology
- Soybean genetics and cultivation
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Genetic Mapping and Diversity in Plants and Animals
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Postharvest Quality and Shelf Life Management
- Plant responses to elevated CO2
- Biofuel production and bioconversion
- Bioenergy crop production and management
- Plant Gene Expression Analysis
- Calpain Protease Function and Regulation
- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Plant Surface Properties and Treatments
- Potato Plant Research
- Microbial Metabolic Engineering and Bioproduction
- Heat shock proteins research
- Nematode management and characterization studies
- Plant responses to water stress
- Plant pathogens and resistance mechanisms
Corteva (United States)
2019-2023
Pioneer Hi-Bred
1997-2018
Sunset Laboratory (United States)
2017
Diversitech (United States)
2003
Purdue University West Lafayette
2003
Iowa State University
1993-1996
Agricultural Research Service
1993
While transformation of the major monocot crops is currently possible, process typically remains confined to one or two genotypes per species, often with poor agronomics, and efficiencies that place these methods beyond reach most academic laboratories. Here, we report a approach involving overexpression maize (Zea mays) Baby boom (Bbm) Wuschel2 (Wus2) genes, which produced high frequencies in numerous previously nontransformable inbred lines. For example, Pioneer PHH5G recalcitrant...
Agriculturally advantageous reduction in plant height is usually achieved by blocking the action or production of gibberellins. Here, we describe a different dwarfing mechanism found maize brachytic2 (br2) mutants characterized compact lower stalk internodes. The these plants results from loss P-glycoprotein that modulates polar auxin transport stalk. sorghum ortholog br2 dwarf3 (dw3), an unstable mutant long-standing commercial interest and concern. A direct duplication within dw3 gene...
Constitutive expression of the
Lack of sufficient water is a major limiting factor to crop production worldwide, and the development drought-tolerant germplasm needed improve productivity. The phytohormone ethylene modulates plant growth as well response abiotic stress. Recent research has shown that modifying biosynthesis signaling can enhance drought tolerance. Here, we report novel negative regulators signal transduction in Arabidopsis (Arabidopsis thaliana) maize (Zea mays). These are encoded by ARGOS gene family. In...
Manipulation of plant natural product biosynthesis through genetic engineering is an attractive but technically challenging goal. Here, we demonstrate that different secondary metabolites can be produced in cultured maize cells by ectopic expression the appropriate regulatory genes. Cell lines engineered to express transcriptional activators C1 and R accumulate two cyanidin derivatives, which are similar predominant anthocyanin found differentiated tissues. In contrast, cell P various...
Endosperm of cereal grains is one the most important renewable resources for food, feed, and industrial raw material. It consists four triploid cell types, i.e., aleurone, starchy endosperm, transfer cells, cells embryo surrounding region. In maize, aleurone layer thick covers perimeter endosperm. Specification maize fate proposed to occur through activation tumor necrosis factor receptor-like receptor kinase CRINKLY4. A second gene essential development defective kernel 1 ( dek1 ). Here we...
We report on the construction of maize minichromosomes using shuttle vectors harboring native centromeric segments, origins replication, selectable marker genes, and telomeric repeats. These were introduced into scutellar cells immature embryos by microprojectile bombardment. Several independent transformation events identified containing in addition to normal diploid complement 20 chromosomes. Immunostaining indicated that recruited protein C, which is a specific component...
The soybean root necrosis ( rn ) mutation causes a progressive browning of the soon after germination that is associated with accumulation phytoalexins and pathogenesis‐related proteins an increased tolerance to root‐borne infection by fungal pathogen, Phytophthora sojae . Grafting decapitation experiments indicate phenotype root‐autonomous at macroscopic level. However, onset severity was modulated in intact plants exposure light, as extent lateral formation, suggesting both roots could be...
Stalk lodging in maize (
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPotato Cultivar Differences Associated with MealinessDiane R. McComber, Harry T. Horner, Mark A. Chamberlin, and David F. CoxCite this: J. Agric. Food Chem. 1994, 42, 11, 2433–2439Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November 1994https://pubs.acs.org/doi/10.1021/jf00047a013https://doi.org/10.1021/jf00047a013research-articleACS PublicationsRequest reuse permissionsArticle...
Anatomical and ultrastructural aspects of soybean embryo, endosperm, ovule development are described for the zygote to late heart-shaped embryo stages (0-35 d postfertilization). Nucellar cells subtending degenerative synergid break down, allowing pollen tube passage this synergid. In 15 17 ovules studied, occupies a position abfunicular zygote. The inner integument differentiates into endothelium exterior layers thick-walled at globular stage. has cuticle on its surface that begins fragment...
Photosynthesizing soybean plants were exposed to 14 CO 2 study the incorporation of labeled water-insoluble photosynthates in ovules at various developmental stages. Using autoradiographic techniques on sectioned material, we show that distribution carbon different ovular tissues is regulated spatially and temporally. During zygote through globular stages embryo development, assimilates accumulate integumentary tissue adjacent micropylar chalazal poles sac. A vascular trace two adfunicular...
Disease lesion mimic mutations that affect aerial parts of plants have been characterized in several species, but root disease mimics not previously described. Our objective was to characterize genetically and physiologically three independently derived necrotic mutants found soybean. The were inherited as a single recessive gene, allelic, linked any seven marker traits tested. When inoculated via the hypocotyl, susceptible hyphal infection by compatible race fungal pathogen Phytophthora...
Long tracts (megatracts) of (CAG)n, (TAG)n, and (GAA)n microsatellite sequences capable forming composite DNA segments were found in the maize (Zea mays L.) genome. Some (CAG)n (TAG)n megatracts organized clusters up to 1 Mb on several chromosomes, as detected by fluorescence situ hybridization (FISH), well extended fibers. Extensive polymorphism was among different inbred lines with respect number size megatract A chromosomes. Polymorphism also common B chromosomes nuclei line Zapalote...
In the current genomic era, search and deployment of new semi-dwarf alleles have continued to develop better plant types in all cereals. We characterized an agronomically optimal mutation Zea mays L. a parallel polymorphism Sorghum bicolor cloned maize brachytic1 (br1-Mu) allele by modified PCR-based Sequence Amplified Insertion Flanking Fragment (SAIFF) approach. Histology RNA-Seq elucidated mechanism semi-dwarfism. GWAS linked sorghum height QTL with Br1 homolog resequencing West African...
A technique was developed for isolating embryo sacs from ovules of soybean and separating endosperm. Image analysis cytophotometry were used to determine the relative mass DNA size nuclei endosperm cells. Analyses done at globular through late heart‐shaped stages correlate ploidy level or nuclear size, differentiation in these tissues. Mean fairly constant all studied. Ploidy condition stable, 95%–99% distributed a bipolar pattern by 2C 4C. Few (3%) had levels above 4C stage. Variability...
Premise of research. A male-sterile, female-sterile mutant was discovered in a w4-m mutable line Glycine max L. The mechanism its sterility not well understood. Therefore, different cytological and microscopic techniques were undertaken to better understand the process phenotype development. Molecular research indicated that mer3 responsible for sterility.Methodology. Macro images collected whole plants, flowers, anthers, pods, ovules. Chromosome spreads from anthers at various meiotic...