A5007843325- Cancer Cells and Metastasis
- Bone health and treatments
- Cancer, Hypoxia, and Metabolism
- Chemokine receptors and signaling
- ATP Synthase and ATPases Research
- Radiopharmaceutical Chemistry and Applications
- Genomics and Chromatin Dynamics
- Mass Spectrometry Techniques and Applications
- Inflammatory mediators and NSAID effects
- MicroRNA in disease regulation
- Ion channel regulation and function
- Histone Deacetylase Inhibitors Research
- 3D Printing in Biomedical Research
- Wnt/β-catenin signaling in development and cancer
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Management of metastatic bone disease
- Cellular Mechanics and Interactions
University of Notre Dame
2018-2024
Mike and Josie Harper Cancer Research Institute
2017-2024
Cancer Research Institute
2020-2021
Abstract Bone is one of the most common sites for metastasis across cancers. Cancer cells that travel through vasculature and invade new tissues can remain in a non-proliferative dormant state years before colonizing metastatic site. Switching from dormancy to colonization rate-limiting step bone metastasis. Here we develop an ex vivo co-culture method grow cancer mouse bones assess cell proliferation using healthy or cancer-primed bones. Profiling soluble factors conditioned media...
Potassium ion channels are critical in the regulation of cell motility. The acquisition motility is an essential parameter cancer metastasis. However, role K+ metastasis has been poorly studied. High expression hG1 gene, which encodes for Kv11.1 channel associates with good prognosis estrogen receptor-negative breast (BC). We evaluated efficacy activator NS1643 arresting a triple negative (TNBC) mouse model. significantly reduces metastatic spread tumors vivo by inhibiting motility,...
Metastasis is responsible for over 90% of cancer-related deaths, and bone the most common site breast cancer metastasis. Metastatic cells home to trabecular bone, which contains hematopoietic stromal lineage in marrow. As such, it crucial understand whether or marrow enhance cell migration toward tissue. To this end, we quantified MDA-MB-231 human two- three-dimensional (3D) environments. First, found that cultured on tissue culture plastic migrated intact explants at a higher rate than...
SummaryIntracellular pH (pHi) dynamics regulate normal cell function, and dysregulated pHi is an emerging hallmark of cancer (constitutively increased pHi) neurodegeneration decreased pHi). However, the molecular mechanisms by which biology are poorly understood. Here, we discovered that altering in human breast epithelial cells triggers global transcriptional changes. We identified 176 genes differentially regulated pHi, with pHi-dependent clustering signaling glycolytic pathways. Using...
Abstract Bone is one of the most common and dangerous sites for metastatic tumor growth across cancer types, including breast cancer. At death, roughly 73% women with have bone metastasis, often growing in highly vascularized bones. These metastases are detrimental to patient's quality life, causing hypercalcemia, acid/base imbalance, aberrant hematopoiesis, immune response, osteolysis (bone loss) resulting severe pain immobility. Current treatment options patients include chemotherapy,...
Summary Intracellular pH (pHi) dynamics regulate normal cell function, and dysregulated pHi is an emerging hallmark of cancer (constitutively increased pHi) neurodegeneration decreased pHi). However, the molecular mechanisms by which biology are poorly understood. Here, we discovered that altering in human breast epithelial cells triggers global transcriptional changes. We identified 176 genes differentially regulated pHi, with pHi-dependent clustering signaling glycolytic pathways. Using...