A5056107740- Infant Nutrition and Health
- Neuroinflammation and Neurodegeneration Mechanisms
- Infant Health and Development
- Child Nutrition and Feeding Issues
- Multiple Sclerosis Research Studies
- Alzheimer's disease research and treatments
- Cancer Genomics and Diagnostics
- Clostridium difficile and Clostridium perfringens research
- Genomics and Rare Diseases
- Probiotics and Fermented Foods
- RNA modifications and cancer
- Chromatin Remodeling and Cancer
- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Oral microbiology and periodontitis research
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Clinical Nutrition and Gastroenterology
- Microscopic Colitis
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Cell Function and Interaction
- Insect symbiosis and bacterial influences
- Mitochondrial Function and Pathology
- Identification and Quantification in Food
- Single-cell and spatial transcriptomics
Nationwide Children's Hospital
2015-2024
The Ohio State University
2016
University of Nevada, Las Vegas
2015
Abstract Objective Epilepsy‐associated developmental lesions, including malformations of cortical development and low‐grade tumors, represent a major cause drug‐resistant seizures requiring surgical intervention in children. Brain‐restricted somatic mosaicism has been implicated the genetic etiology these lesions; however, many contributory genes remain unidentified. Methods We enrolled 50 children who were undergoing epilepsy surgery into translational research study. Resected tissue was...
One significant drawback of current probiotic therapy for the prevention necrotizing enterocolitis (NEC) is need at least daily administration because poor persistence after enteral administration, increasing risk bacteria causing bacteremia or sepsis if intestines are already compromised. We previously showed that effectiveness Lactobacillus reuteri ( Lr) in preventing NEC enhanced when Lr grown as a biofilm on surface dextranomer microspheres (DM). Here we sought to test efficacy by...
Periodontal disease exemplifies a chronic and recurrent infection with necessary biofilm component. Mucosal inflammation is hallmark response of the host seen in diseases, such as colitis, gingivitis, periodontitis (and related disorder peri-implantitis). We have taken advantage our recently developed rat model human peri-implantitis that recapitulates osteolysis, requirement formation, perpetuation bona fide state, to test new therapeutic modality two novel components. First we used...
As with all orally consumed probiotics, the Gram-positive bacterium Lactobacillus reuteri encounters numerous challenges as it transits through gastrointestinal tract of host, including low pH, effectors host immune system, well competition commensal and pathogenic bacteria, which can greatly reduce availability live bacteria for therapeutic purposes. Recently we showed that L. reuteri, when adhered in form a biofilm to semi-permeable biocompatible dextranomer microsphere, reduces incidence...
For prophylactic therapy, mice received an oral antibiotic cocktail followed by clindamycin injection, probiotic administration (planktonic vs. biofilm state), C. difficile gavage. treatment antibiotics and first, administration. Clinical sickness scores (CSS) intestinal histologic injury (HIS) were assigned.In the Prophylactic Therapy model, CSS: 67% of untreated exposed to demonstrated CSS ≥ 6, which is consistent with infection (p< .001 compared unexposed mice). In treated planktonic Lr,...
Background:Clostridium difficile infection is the most common cause of antimicrobial-associated diarrhea. Our aim was to introduce a novel and efficient clinical sickness score (CSS), define detailed histologic injury (HIS) in murine model C. colitis. Methods: Mice received an antibiotic cocktail (kanamycin, gentamicin, colistin, metronidazole, vancomycin) for 96 h. After 48 h, mice intraperitoneal injection clindamycin, followed by oral (1.5 × 107 CFU). Signs were scored using CSS (range...
Somatic variants are a major cause of human disease, including neurological disorders like focal epilepsies, but can be challenging to study due their mosaicism in bulk tissue biopsies. Coupling single-cell genotype and transcriptomic data has potential provide insight into the role somatic play disease etiology, such as by determining what cell types affected or how mutations affect gene expression. Here, we asked whether commonly used single-nucleus 3'- 5'-RNA-sequencing assays derive for...
Abstract Rasmussen encephalitis (RE) is a rare childhood neurological disease characterized by progressive unilateral loss of function, hemispheric atrophy and drug-resistant epilepsy. Affected brain tissue shows signs infiltrating cytotoxic T-cells, microglial activation, neuronal death, implicating an inflammatory process. Recent studies have identified molecular correlates inflammation in RE, but cell-type-specific mechanisms remain unclear. We used single-nucleus RNA-sequencing...
Abstract Glioblastoma, the most common primary brain malignancy in adults, is an aggressive cancer with five-year median survival of ~6.8%. New treatment options are urgently needed for glioblastoma and other high-grade gliomas (HGGs). Oncolytic viruses (OVs) emerging class therapy currently being evaluated as treatments a variety malignancies, including glioblastoma. Our labs developed OV C134, now under evaluation phase I clinical trial recurrent (NCT03657576). In addition to directly...
Abstract Epilepsy-associated developmental lesions, including malformations of cortical development and low-grade tumors, represent a major cause drug-resistant seizures requiring surgical intervention in children. Brain-restricted somatic mosaicism has been implicated the genetic etiology these lesions; however, many contributory genes remain unidentified. We enrolled 50 children undergoing epilepsy surgery into translational research study. performed exome RNA-sequencing resected brain...