A5014762926- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- Advanced Biosensing Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- RNA regulation and disease
- Monoclonal and Polyclonal Antibodies Research
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
- Gene Regulatory Network Analysis
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Multiple Myeloma Research and Treatments
- T-cell and B-cell Immunology
- Chronic Myeloid Leukemia Treatments
- Connective tissue disorders research
- Advanced Fluorescence Microscopy Techniques
- Genomics and Rare Diseases
- Lipid Membrane Structure and Behavior
- Microbial metabolism and enzyme function
- DNA and Nucleic Acid Chemistry
Science for Life Laboratory
2016-2020
Karolinska Institutet
2016-2020
West Pomeranian University of Technology
2016
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ Sequencing (BLISS), featuring following: (1) direct labelling DSBs fixed cells tissue sections on a solid surface; (2) low-input requirement by linear amplification tagged vitro transcription; (3) quantification through unique...
The spatial distribution of cell surface proteins governs vital processes the immune system such as intercellular communication and mobility. However, fluorescence microscopy has limited scalability in multiplexing throughput needed to drive proteomics discoveries at subcellular level. We present Molecular Pixelation (MPX), an optics-free, DNA sequence-based method for single cells using antibody-oligonucleotide conjugates (AOCs) DNA-based, nanometer-sized molecular pixels. relative...
Abstract The spatial distribution of cell surface proteins govern vital processes the immune system such as inter-cell communication and mobility. However, tools for studying these at high multiplexing scale, resolution, throughput needed to drive novel discoveries are lacking. We present Molecular Pixelation, a DNA-sequencing based method single analysis quantify protein abundance, distribution, colocalization targeted using Antibody Oligonucleotide Conjugates (AOCs). Relative locations...
Abstract With the exception of lamina-associated domains, radial organization chromatin in mammalian cells remains largely unexplored. Here, we describe genomic loci positioning by sequencing (GPSeq), a genome-wide method for inferring distances to nuclear lamina all along radius that works gradual enzymatic restriction from towards nucleus center, followed generated cut sites. Using GPSeq, mapped human genome at 100 kb resolution, which revealed patterns and epigenomic features, gene...
Abstract We present a method for genome-wide DNA double-strand Breaks (DSBs) Labeling In Situ and Sequencing (BLISS) which, compared to existing methods, introduces several key features: 1) high efficiency low input requirement by in situ DSB labeling cells or tissue sections directly on solid surface; 2) easy scalability performing reactions multi-well plates; 3) sensitivity linearly amplifying tagged DSBs using vitro transcription; 4) accurate quantification control of PCR biases unique...
Advances in spatial proteomics and protein colocalization are a driving force the understanding of cellular mechanisms their influence on biological processes. New methods field call for development algorithms open up new avenues research. The newly introduced Molecular Pixelation (MPX) provides information surface proteins relationship with each other single cells. This allows silico representation neighborhoods membrane as graphs. In order to analyze this data modality, we adapted local...
Abstract Precisely measuring the location and frequency of DNA double-strand breaks \(DSBs) along genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity, or practicality. Here, we present Breaks Labeling _In Situ_ Sequencing \(BLISS), featuring: 1) direct labeling DSBs fixed cells tissue sections on a solid surface; 2) low-input requirement by linear amplification tagged _in vitro_ transcription; 3) quantification through unique...