A5086775172- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Gene expression and cancer classification
- Glycosylation and Glycoproteins Research
- Advanced Fluorescence Microscopy Techniques
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- CAR-T cell therapy research
- Advanced Proteomics Techniques and Applications
- Cancer Cells and Metastasis
- bioluminescence and chemiluminescence research
- HER2/EGFR in Cancer Research
- Click Chemistry and Applications
- Cancer Genomics and Diagnostics
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Image Processing and 3D Reconstruction
- Image Processing Techniques and Applications
- Nanoplatforms for cancer theranostics
- Nanowire Synthesis and Applications
- Luminescence and Fluorescent Materials
- AI in cancer detection
- Cancer Research and Treatments
490 BioTech (United States)
2022
Quantitative BioSciences
2017-2020
Oregon Health & Science University
2017-2018
Cernostics (United States)
2011
Carnegie Mellon University
1989-1991
Many critical advances in research utilize techniques that combine high-resolution with high-content characterization at the single cell level. We introduce MICS (MACSima Imaging Cyclic Staining) technology, which enables immunofluorescent imaging of hundreds protein targets across a specimen subcellular resolution. is based on cycles staining, imaging, and erasure, using photobleaching fluorescent labels recombinant antibodies (REAfinity Antibodies), or release (REAlease Antibodies) their...
Extrinsic signals are implicated in breast cancer resistance to HER2-targeted tyrosine kinase inhibitors (TKIs). To examine how microenvironmental influence resistance, we monitored TKI-treated cell lines grown on microenvironment microarrays composed of printed extracellular matrix proteins supplemented with soluble proteins. We tested ∼2,500 combinations 56 and 46 basal-like HER2+ (HER2E) or luminal-like (L-HER2+) cells treated the TKIs lapatinib neratinib. In HER2E cells, hepatocyte...
Abstract The formation of lamellipodia in migrating cells involves dynamic processes that occur a cyclic manner as the leading edge cell slowly advances. We used video‐enhanced contrast microscopy (VEC) to monitor motile behavior classify protrusions into temporal stages initial and established (Fisher et al.: Cell Motility Cytoskeleton 11:235–247, 1988), fixation cells. Multiple parameter fluorescence imaging methods (DeBiasio Journal Biology 105:1613–1622, 1987; Waggoner Methods Biology,...
Abstract A number of highly multiplexed immunostaining and imaging methods have advanced spatial proteomics cancer for improved treatment strategies. While a variety been developed, the most widely used are limited by harmful signal removal techniques, difficulties with reagent production antigen sensitivity. Multiplexed employing oligonucleotide (oligos)-barcoded antibodies is an alternative approach that growing in popularity. However, challenges remain consistent conjugation oligos to...
Abstract A system is described for performing multicolor fluorescence image cytometry of cell preparations. After the setting up stage, operation automatic: microscope fields are found and focused; then images acquired each fluorophore, corrected analyzed, without any operator interaction. Human peripheral blood lymphocytes on slides were used as a test system. In these experiments, three fluorescent antibodies to identify lymphocyte sub‐populations, DNA content probe was all nucleated...
Multicolor microscopy tools necessary to localize and visualize the complexity of subcellular systems are limited by current fluorophore technology. While commercial fluorophores cover spectral space from ultraviolet near infrared region optimized for conventional bandpass based fluorescence microscopy, they not ideal highly multiplexed as tend have short Stokes shifts, restricting number that can be detected in a single sample four five. Herein, we synthesized library 95 novel...
Advanced genetic characterization has informed cancer heterogeneity and the challenge it poses to effective therapy; however, current methods lack spatial context, which is vital successful therapy. Conventional immunolabeling, commonplace in clinic, can provide context protein expression. However, these techniques are spectrally limited, resulting inadequate capacity resolve heterogenous cell subpopulations within a tumor.We developed optimized oligonucleotide conjugated antibodies...
Abstract Persistently high, worldwide mortality from cancer highlights the unresolved challenges of disease surveillance and detection that impact survival. Development a non-invasive, blood-based biomarker would transform survival cancer. We demonstrate functionality ultra-high content analyses newly identified population tumor cells are hybrids between neoplastic immune in patient matched peripheral blood specimens. Using oligonucleotide conjugated antibodies (Ab-oligo) permitting cyclic...
Understanding the impact of microenvironment on phenotype cells is a difficult problem due to complex mixture both soluble growth factors and matrix-associated proteins in vivo. Furthermore, readily available reagents for modeling microenvironments vitro typically utilize mixtures that are incompletely defined suffer from batch variability. The microarray (MEMA) platform allows assessment thousands simple combinations their cellular phenotypes single assay. MEMAs prepared well plates, which...
For many years pathologists have used Hematoxylin and Eosin (H&E), single marker immunohistochemistry (IHC) in situ hybridization with manual analysis by microscopy or at best simple digital imaging. There is a growing trend to update pathology workflow improve objectivity productivity, as has been done radiology. also need for tissue-based multivariate biomarker assays the accuracy of diagnostic, prognostic, predictive testing. Multivariate tests are not compatible traditional marker,...
We describe here a method, termed immunoFISH, for simultaneous in situ analysis of the composition and distribution proteins individual RNA transcripts single cells. Individual molecules are labeled by hybridization target concurrently stained using immunofluorescence. Multicolor fluorescence images acquired analyzed to determine abundance, composition, hybridized probes assessed ability immunoFISH quantify protein transcript levels cultured HER2 positive breast cancer cells human tumor...
The Automated Interactive Microscope is a robotic light microscope workstation that combines high performance microscopy and computing to explore the chemical molecular dynamics of cells tissues.
Successful cancer treatment continues to elude modern medicine and its arsenal of therapeutic strategies. Therapy resistance is driven by significant tumor heterogeneity, complex interactions between malignant, microenvironmental immune cells cross talk signaling pathways. Advances in molecular characterization technologies such as next generation sequencing have helped unravel this network vastly affected how diagnosed treated. However, the translation genomic analyses pathological...
Stress fibers in serum-deprived fibroblasts provide an excellent system for studying the assembly dynamics of actin-based cytoskeleton. Previous studies suggested that containing actin and myosin II are restructured via two alternative pathways: coupled solation-contraction disassembly without contraction. The former process appears to be regulated by Ca++, but regulation latter has not been extensively explored. To assess possible role protein phosphorylation, we examined dynamic behavior...
Digital Imaging Light Microscopy technology has made major advancements over the past years. Changes have included significant improvements in sensitivity and image quality, but also advent of innovative methods that greatly increased versatility instruments. Methods to yield contrast specimen leaps specificity signal-to-noise ratio. The combination these advanced technologies now permits acquisition 2 3 dimensional data, up five independently labeled parameters, fixed specimens, or - time...
Journal Article Antibody Conjugated Oligonucleotides as a Platform for Cyclic Immunofluorescent Staining Get access Nathan McMahon, McMahon Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, USA Corresponding author: mcmahonn@ohsu.edu Search other works by this author on: Oxford Academic Google Scholar Jocelyn Jones, Jones Allison Solanki, Solanki Jennifer Eng, Eng Sunjong Kwon, Kwon Young-Hwan Chang, Chang USAComputational Biology Program, Guillaume...
Understanding the impact of microenvironment on phenotype cells is a difficult problem due to complex mixture both soluble growth factors and matrix-associated proteins in vivo. Furthermore, readily available reagents for modeling microenvironments vitro typically utilize mixtures that are incompletely defined suffer from batch variability. The microarray (MEMA) platform allows assessment thousands simple combinations their cellular phenotypes single assay. MEMAs prepared well plates, which...
Abstract Cell lines represent a valuable model system for the study of breast cancer, as they capture cellular diversity, mutational spectrum, expression subtypes, and genomic alterations that are observed in clinical specimens. However, like any system, cell imperfect, particularly when it comes to capturing effects myriad signals interactions encounter their microenvironment (ME). We utilizing technology known microarrays (MEMA) begin unravel consequences cancer cells with ME. MEMA consist...