A5059069455- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- RNA modifications and cancer
- Hematopoietic Stem Cell Transplantation
- Cancer Cells and Metastasis
- RNA Research and Splicing
- Zebrafish Biomedical Research Applications
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Cytomegalovirus and herpesvirus research
- Liver physiology and pathology
- Cancer-related Molecular Pathways
- Lymphatic System and Diseases
- Cancer-related gene regulation
- TGF-β signaling in diseases
- Lymphoma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- Hippo pathway signaling and YAP/TAZ
- Toxoplasma gondii Research Studies
- Chromosomal and Genetic Variations
- Cell death mechanisms and regulation
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer, Hypoxia, and Metabolism
Stanford University
2012-2024
Oregon Health & Science University
2003-2010
OHSU Knight Cancer Institute
2009
University of Portland
2006
Indiana University – Purdue University Indianapolis
2004
Indiana University School of Medicine
2004
The switch from mitosis to meiosis is the key event marking onset of differentiation in germline stem cell lineage. In Drosophila, translational repressor Bgcn required for spermatogonia stop and transition meiotic prophase spermatocyte state. Here we show that mammalian homolog YTHDC2 facilitates a clean mouse germ cells, revealing conserved role this critical fate transition. YTHDC2-deficient male cells enter but have mixed identity, maintaining expression Cyclin A2 failing properly...
Transplanted adult bone marrow-derived cells (BMDCs) have been shown to adopt the phenotype and function of several nonhematopoietic cell lineages promote tumorigenesis. Beyond its cancer enhancing potential, fusion has recently emerged as an explanation how BMDCs regenerate diseased heptocytes, contribute Purkinje neurons skeletal cardiac muscle cells, participate in skin heart regeneration. Although epithelial also observed intestine, a mechanism not investigated. Here, we show that...
Despite an important role in vascular development and repair, the origin of endothelial progenitors remains unknown. Accumulating evidence indicates that cells derived from hematopoietic system participate angiogenesis. However, identity functional these remain controversial. Here we show can differentiate common myeloid granulocyte/macrophage progenitors. Endothelial transplanted bone marrow-derived lineage expressed CD31, von Willebrand factor, Tie2 but did not express markers CD45 F4/80...
Although the lymphatic system arises as an extension of venous vessels in embryo, little is known about role circulating progenitors maintenance or development endothelium. Here, we investigated whether hematopoietic stem cells (HSCs) have potential to give rise endothelial (LEC).Following transfer marked HSCs into irradiated recipients, donor-derived LEC that co-express markers Lyve-1 and VEGFR-3 were identified several tissues. HSC-derived persisted for more than 12 months contributed...
Posttranscriptional regulation of gene expression by RNA-binding proteins can enhance the speed and robustness cell state transitions controlling RNA stability, localization, or if, when, where mRNAs are translated. The helicase YTHDC2 is required to shut down components mitotic program facilitate a proper switch from mitosis meiosis in mouse germ cells. Here, we show that has second essential role promoting meiotic progression late spermatocytes. Inducing conditional knockout Ythdc2 during...
The expression of ASPP2 (53BP2L), a proapoptotic member family p53-binding proteins, is frequently suppressed in many human cancers. Accumulating evidence suggests that inhibits tumor growth; however, the mechanisms by which suppresses formation remain to be clarified. To study this, we targeted allele mouse replacing exons 10-17 with neoR gene. ASPP2(-/-) mice were not viable because an early embryonic lethal event. Although ASPP2(+/-) appeared developmentally normal, they displayed...
In mantle cell lymphoma (MCL) and some cases of multiple myeloma (MM), cyclin D1 expression is deregulated by chromosome translocations involving the immunoglobulin heavy chain (IgH) locus. To evaluate mechanisms responsible, gene targeting was used to study long-distance regulation. Remarkably, these targeted lines lost translocated (t(11;14)). MCL MM cells, nonrearranged (CCND1) locus reverts from CpG hypomethylated hypermethylated. Reintroduction induced a loss methylation at unrearranged...
ABSTRACT Post-transcriptional regulation of gene expression by RNA-binding proteins can enhance the speed and robustness cell state transitions controlling RNA stability, localization, or if, when where mRNAs are translated. The helicase YTHDC2 is required to shut down components mitotic program facilitate a proper switch from mitosis meiosis in mouse germ cells. Here we show that has second essential role promoting meiotic progression late spermatocytes. Inducing conditional knockout Ythdc2...
During development, cells must quickly switch from one cell state to the next execute precise and timely differentiation. One method ensure fast transitions in states is by controlling gene expression at post-transcriptional level through action of RNA-binding proteins on mRNAs. The ability accurately identify RNA targets specific stages key understanding functional role during development. Here we describe an adapted formaldehyde immunoprecipitation (fRIP) protocol