A5100778242- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Microplastics and Plastic Pollution
- Enzyme Catalysis and Immobilization
- biodegradable polymer synthesis and properties
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Food composition and properties
- Protein purification and stability
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Hydrolysis and Bioactive Peptides
- Amino Acid Enzymes and Metabolism
- Phytase and its Applications
- Microbial Metabolic Engineering and Bioproduction
- Animal Genetics and Reproduction
- Effects and risks of endocrine disrupting chemicals
- CRISPR and Genetic Engineering
- Enzyme-mediated dye degradation
- Biochemical and Structural Characterization
- Carbohydrate Chemistry and Synthesis
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
Jiangnan University
2016-2025
State Key Laboratory of Food Science and Technology
2016-2025
Huashan Hospital
2024
Fudan University
2024
Wuhan Polytechnic University
2012-2023
Shaoyang University
2021
University of Michigan
2003-2019
Tarim University
2019
Michigan Medicine
2017
Dong-A University
2008-2015
Abstract The coexistence of cholecystokinin‐octapeptide‐like (CCK‐L) and/or vasoactive‐intestinal‐polypeptide‐like immunoreactive (VIP‐LI) materials and glutamate decarboxylase (GAD) was studied in the rat hippocampus dentate gyrus by means immunohistochemistry. Consecutive 40‐μm‐thick sections were incubated different antisera those cells which bisected plane sectioning so as to be included at paired surfaces two adjacent identified. immunoreactivities for these peptides GAD same cell could...
d-Tagatose, a rare sugar endowed with low-calorie property, superior taste quality, and probiotic functionality, has garnered significant research attention. However, the prevailing biological production methods relying on β-galactosidase l-arabinose isomerase face challenges including high cost suboptimal conversion efficiency. Consequently, it is of great significance to find efficient alternative routes for d-tagatose synthesis. Previously, Thermotoga petrophila tagaturonate 3-epimerase...
Cutinase, which exists in both fungi and bacteria, catalyzes the cleavage of ester bonds cutin. Fungal cutinases have been extensively studied, however, reports on bacterial limited due to lack knowledge concerning identity their open reading frames. In present study, cutinase from Thermobifida fusca was induced by cutin purified homogeneity following p-nitrophenyl butyrate hydrolyzing activity. Peptide mass fingerprinting analysis wild-type enzyme matched two proteins, Tfu_0883 Tfu_0882,...
A cutinase from Thermomyces cellullosylitica (Thc_Cut1), hydrolyzing the synthetic polymer polyethylene terephthalate (PET), was fused with two different binding modules to improve sorption and thereby hydrolysis. The were cellobiohydrolase I Hypocrea jecorina (CBM) a polyhydroxyalkanoate depolymerase Alcaligenes faecalis (PBM). Although both have hydrophobic nature, it possible express proteins in E. coli. Both fusion enzymes native one had comparable kcat values range of 311 342 s–1 on...
Abstract A bacterial cutinase from Thermobifida fusca , named Tfu_0883, was genetically modified by site‐directed mutagenesis to enhance its activity on poly(ethylene terephthalate) (PET). The new mutations tailored the catalytic site for PET, increasing affinity of this hydrophobic substrate and ability hydrolyze it. mutation I218A designed create space double Q132A/T101A both increase hydrophobicity. mutant soluble p ‐nitrophenyl butyrate increased two‐fold compared wild‐type cutinase,...
Abstract Bacillus subtilis ATCC 6051a is an undomesticated strain used in the industrial production of enzymes. Because it poorly transformable, genetic manipulation this requires a highly efficient genome editing method. In study, Streptococcus pyogenes CRISPR/Cas9 system consisting all-in-one knockout plasmid containing target-specific guide RNA, cas9 and homologous repair template was established for gene disruption B. 6051a. With efficiency 33% to 53%, disrupt srfC , spoIIAC nprE aprE...
We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This is capable of robust recombinant protein production amenable to high-cell-density fermentation. Because promoter among factors that influence target proteins, optimization initial promoter, PamyQ amyloliquefaciens, should improve expression using this strain. study was undertaken develop new, high-level system in B. CCTCC 2016536.Using enzyme...
Derivatives of C60 have been shown to be effective free radical scavengers. Hence, many the biological functions fullerene are believed due their antioxidant properties. Here we present evidence show that fullerenols, caged oxides, exert neuroprotective by blocking glutamate receptors and lowering intracellular calcium, [Ca2+]i. In neuronal cultures, fullerenols reduce glutamate-induced neurotoxicity about 80% at 50μM. No significant effect was observed on H2O2/Fe2+-induced under same...
Pullulanase (EC 3.2.1.41) is a well-known starch-debranching enzyme. Its instability and low catalytic efficiency are the major factors preventing its widespread application. To address these issues, Asp437 Asp503 of pullulanase from Bacillus deramificans were selected in this study as targets for site-directed mutagenesis based on structure-guided consensus approach. Four mutants (carrying mutations D503F, D437H, D503Y, D437H/D503Y) generated characterized detail. The results showed that...
Chloramphenicol and florfenicol are broad-spectrum antibiotics. Although the bacterial resistance mechanisms to these antibiotics have been well documented, hydrolysis of has not reported in detail. This study reports two by a specific hydrolase that is encoded gene identified from soil metagenome. Hydrolysis chloramphenicol recognized cell extracts Escherichia coli expressing acetate esterase gene, estDL136. A hydrolysate was as p-nitrophenylserinol liquid chromatography-mass spectroscopy...
Cutinase is a multifunctional esterase with potential industrial applications. In the present study, truncated version of extracellular Thermobifida fusca cutinase without signal peptide (referred to as NS ) was heterologously expressed in Escherichia coli BL21(DE3). The results showed that majority activity located culture medium. 3-liter fermentor, medium reached 1,063.5 U/ml (2,380.8 mg/liter), and productivity 40.9 U/ml/h. Biochemical characterization purified it has enzymatic properties...