A5082661945- Genomic variations and chromosomal abnormalities
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- Immunodeficiency and Autoimmune Disorders
- Physical Education and Training Studies
- Genetic factors in colorectal cancer
- Nicotinic Acetylcholine Receptors Study
- Monoclonal and Polyclonal Antibodies Research
- Advanced X-ray and CT Imaging
- Cancer-related Molecular Pathways
- Neurogenetic and Muscular Disorders Research
- RNA Research and Splicing
- Human Health and Disease
- Cancer Genomics and Diagnostics
- Congenital heart defects research
- Advanced Memory and Neural Computing
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Neuropeptides and Animal Physiology
- Chronic Lymphocytic Leukemia Research
- Collagen: Extraction and Characterization
- Cell Adhesion Molecules Research
Duke University
2021-2024
Duke University Hospital
2021-2024
Duke Medical Center
2021-2024
Washington University in St. Louis
2013
University of Massachusetts Chan Medical School
2013
Salisbury NHS Foundation Trust
2012
University of Massachusetts Amherst
2011
Royal College of Surgeons of England
1997
The Honourable Society of Lincoln's Inn
1987-1997
Queen Mary Hospital
1997
Zinc finger nucleases (ZFNs) and transcription activator-like effector (TALENs) provide powerful platforms for genome editing in plants animals. Typically, a single nuclease is sufficient to disrupt the function of protein-coding genes through introduction microdeletions or insertions that cause frameshifts within an early coding exon. However, interrogating cis -regulatory modules noncoding RNAs many instances requires excision this element from genome. In human cell lines invertebrates,...
Cys 2 -His zinc finger proteins (ZFPs) are the largest group of transcription factors in higher metazoans. A complete characterization these ZFPs and their associated target sequences is pivotal to fully annotate transcriptional regulatory networks metazoan genomes. As a first step this process, we have characterized DNA-binding specificities 129 sets from Drosophila using bacterial one-hybrid system. This data set contains for at least one encoded ZFP 70 unique genes 23 alternate splice...
The integrated stress response (ISR) maintains proteostasis by modulating protein synthesis and is important in synaptic plasticity, learning, memory. We developed a reporter, SPOTlight, for brainwide imaging of ISR state with cellular resolution. Unexpectedly, we found class neurons mouse brain, striatal cholinergic interneurons (CINs), which the was activated at steady state. Genetic pharmacological manipulations revealed that signaling necessary CINs normal type 2 dopamine receptor (D2R)...
This protocol contains the steps taken for live tissue preparation and two-photon imaging.
Zinc-finger nucleases (ZFNs) have been used for genome engineering in a wide variety of organisms; however, it remains challenging to design effective ZFNs many genomic sequences using publicly available zinc-finger modules.This limitation is part because potential finger-finger incompatibility generated on assembly modules into arrays (ZFAs).Herein, we describe the validation new set two-finger that can be building ZFAs via conventional methods or strategy-finger stitching-that increases...
We have previously assigned the gene for alpha 1 chain of type IV collagen to chromosome 13. In this report we show that coding second heterotrimer is on same chromosome. This first example genes both chains one molecule being syntenic.