A5027030423- Salmonella and Campylobacter epidemiology
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Virus-based gene therapy research
- Ocular Diseases and Behçet’s Syndrome
- Blood properties and coagulation
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Probiotics and Fermented Foods
- Fungal Infections and Studies
- Cutaneous lymphoproliferative disorders research
- Herpesvirus Infections and Treatments
- Systemic Lupus Erythematosus Research
- Glycosylation and Glycoproteins Research
- Poxvirus research and outbreaks
- Dermatology and Skin Diseases
- Blood Coagulation and Thrombosis Mechanisms
- vaccines and immunoinformatics approaches
- Retinal and Optic Conditions
- Respiratory viral infections research
- T-cell and Retrovirus Studies
- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Polysaccharides and Plant Cell Walls
- Vector-Borne Animal Diseases
Nara Women's University
2024
University of Cape Town
2020-2022
Fujita Health University
2021
South African Radio Astronomy Observatory
2021
AstraZeneca (United States)
2020
Iwate Medical University
2020
Tokyo University of Marine Science and Technology
2015
Center for Seeds and Seedlings
2012
Kindai University
2012
Chiba University
1994-2012
Abstract The SARS-CoV-2 Omicron variant (B.1.1.529) has multiple spike protein mutations 1,2 that contribute to viral escape from antibody neutralization 3–6 and reduce vaccine protection infection 7,8 . extent which other components of the adaptive response such as T cells may still target severe outcomes is unknown. Here we assessed ability react in participants who were vaccinated with Ad26.CoV2.S or BNT162b2, unvaccinated convalescent COVID-19 patients ( n = 70). Between 70% 80% CD4 +...
Bakuchiol was isolated from the seeds of Psoralea corylifolia, a tree native to China with various uses in traditional medicine, followed by extraction ether and column chromatography combined silica gel octyldecyl silane. In this study, antimicrobial activities bakuchiol against some oral microorganisms were evaluated vitro. The cell growth Streptococcus mutans inhibited concentration-dependent manner, S. completely prevented 20 microg per ml. bactericidal effect on dependent temperature...
Summary The SARS-CoV-2 Omicron variant has multiple Spike (S) protein mutations that contribute to escape from the neutralizing antibody responses, and reducing vaccine protection infection. extent which other components of adaptive response such as T cells may still target severe outcomes is unknown. We assessed ability react with spike in participants who were vaccinated Ad26.CoV2.S or BNT162b2, unvaccinated convalescent COVID-19 patients (n = 70). found 70-80% CD4 CD8 cell was maintained...
Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exposures, from infection or vaccination, can potently boost spike antibody responses. Less is known about the impact of repeated exposures on T cell Here, we compare prevalence and frequency peripheral SARS-CoV-2-specific immunoglobulin G (IgG) responses in 190 individuals with complex SARS-CoV-2 exposure histories. As expected, an increasing number significantly enhances magnitude IgG responses, while improve responders...
Purified rabbit antibodies to neutral glycolipids were analyzed for their binding mouse embryonal carcinoma cells (ECC) and preimplantation embryos. Antibodies globotetraosylceramide first bind 2 4-cell embryos reach a peak of staining intensity with morulae, whereas anti-Forssman late morulae then, most intensely, early blastocysts. We compared the monoclonal antibody that show although they react similarly ECC, do not so morulae: The stained weakly unevenly, antiserum produced uniformly...
Liquiritigenin (LG), 2, 3-dihydro-7-hydroxy-2-(4-hydroxyphenyl)-(S)-4H-1-benzopyran-4-1, is metabolized to five kinds of conjugates (glucuronides and sulfates) that are excreted predominantly into the bile (Shimamura et al., 1993). Using LG as a model compound, we studied multiplicity for biliary excretion in vivo. was administered i.v. bolus Sprague-Dawley rats (SD rats) received infusions inhibitors [glycyrrhizin (GR), dibromosulfophthalein (DBSP) indocyanine green (ICG)] at their maximal...
Fibroblast growth factor-2 (FGF2) is a potent mitogen for vascular endothelial cells and exogenous administration of FGF2 stimulates angiogenesis. However, increased expression in the retina does not cause One possible explanation that may be capable initiating angiogenesis unless it administered pharmacologic levels or there coexpression another angiogenic factor. Alternatively, control mechanisms sequester vivo, preventing from manifesting its vitro activity. We tested first hypothesis by...
Salmonella enterica serovar Typhimurium has been reported to induce apoptosis in infected macrophages within 14 h from the time of infection by a caspase-1-dependent mechanism. Here, we demonstrate that depletion Lon protease induces rapid and massive mechanism involving both caspases-1 -3. This excessive induction was abrogated disruption invF, which is required for expression pathogenicity island 1 (SPI1) genes. Expression hilA, central regulator SPI1 transcription, repressed after...
The actin-binding protein p57, a member of the coronin family, is expressed in variety immune cells. It has five WD repeats and coiled-coil motif containing leucine zipper, both which are known to mediate protein–protein interactions. In order identify precise regions assess contribution these structural motifs, we prepared various truncated p57 as fusion proteins with glutathione S-transferase (GST) examined their activity. A co-sedimentation assay demonstrated that p571—371 (C-terminal...
Urinary collagen peptides, the breakdown products of endogenous collagen, have been used as biomarkers for various diseases. These non-invasive are easily measured via mass spectrometry, aiding in diagnostics and therapy effectiveness.
Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vectors, to target both LSDV, well other pathogens, including human infectious agents. Historically, these and recombinants were generated in primary (lamb) testis (LT) cells, Madin-Darby bovine kidney (MDBK) cells or eggs. Growth eggs is a laborious process, the use has potential introduce pathogens MDBK are known harbor viral diarrhea (BVDV). In this study, data presented show...
Dual vaccines (n = 6) against both lumpy skin disease (LSD) and bovine ephemeral fever (BEF) were constructed, based on the BEFV glycoprotein (G) gene, with or without matrix (M) protein inserted into one of two different LSDV backbones, nLSDV∆SOD-UCT nLSDVSODis-UCT. The gene cassettes confirmed by PCR; was shown to be expressed immunofluorescence. candidate dual initially tested in a rabbit model; neutralization assays using South African vaccine (B-Phemeral) strain showed an consensus G...
ABSTRACT A phase III observational study evaluating a single‐dose of an inactivated, split‐virus, unadjuvanted AH1pdm vaccine in HCW was conducted. safe and effective needed after the emergence April 2009. We analyzed immunogenicity safety vaccine. total 409 subjects were enrolled given 15 μg hemagglutinin antigen by s.c. injection. Antibody titers measured using hemagglutination‐inhibition antibody assays before vaccination 28 days after. The co‐primary end‐points proportion with 1:40 or...
Abstract Generalized pustular psoriasis (GPP) is a rare form of characterized by the presence variable numbers sterile pustules appearing in erythematous and scaly lesions, which are associated with moderate to severe constitutional symptoms. It can be life‐threatening especially elderly; therefore, medical care must performed rapid succession treatment refractory cases. We have granulocyte monocyte adsorption apheresis (GCAP) on three GPP cases several systemic laboratory findings. As...
The fate of Salmonella enterica serovar Enteritidis (SE) in whole, unbroken eggs was monitored during storage at fixed or changing temperatures after inoculation with 20-47 c.f.u. SE. Eggs stored 10 degrees C and 20 showed little no bacterial growth over 6 weeks, while egg 30 increased the percentage that contained >10(6) 3 weeks. Egg for 5 days followed by caused only a few 2 whereas 22-30 27-35 25 induced rapid increase 1 respectively. Therefore, can control SE growth, although temperature...
The type-specific capsular polysaccharide antigen of Streptococcus agalactiae has in previous experimental studies been considered a significant antiphagocytic factor, whereas the lipoteichoic acid moiety suggested to be factor adherence human fetal cell lines. Since epidemiological data concerning these constituents strains from genital tract are lacking, we attempted serotyping and analysis 100 vaginal isolates. level was shown amount sialic that occupied terminal side chains...
Bovine leukaemia virus (BLV) causes chronic lymphoproliferative disorder and fatal lymphosarcoma in cattle, leading to significant economic losses the beef dairy industries. BLV is endemic globally eleven genotypes have been identified. To date, only Zambian isolates genotyped from Africa. Although high prevalence has reported South Africa, there no molecular characterisation of African isolates. characterise Africa for first time, we investigated phylogenetic relationships compared genetic...