A5063011603- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant biochemistry and biosynthesis
- Light effects on plants
- Plant nutrient uptake and metabolism
- Plant-Microbe Interactions and Immunity
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Plant Virus Research Studies
- Plant Micronutrient Interactions and Effects
- Phytochemicals and Antioxidant Activities
- Plant responses to water stress
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Plant Pathogens and Resistance
- Biochemical and biochemical processes
- Microtubule and mitosis dynamics
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
- Plant responses to elevated CO2
- DNA Repair Mechanisms
Nanjing Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2019-2025
State Forestry and Grassland Administration
2023-2025
Inner Mongolia People's Hospital
2024
Zhongshan Hospital
2023-2024
Xian Yang Central Hospital
2016
Shangqiu Normal University
2015
Peking University
2011-2014
Abstract Chrysanthemum ( morifolium Ramat.) is a globally important ornamental plant with great economic, cultural, and symbolic value. However, research on chrysanthemum challenging due to its complex genetic background. Here, we report near-complete assembly annotation for C. comprising 27 pseudochromosomes (8.15 Gb; scaffold N50 of 303.69 Mb). Comparative evolutionary analyses reveal whole-genome triplication (WGT) event shared by species approximately 6 million years ago (Mya) the...
Background Simple sequence repeats (SSRs) are ubiquitous in eukaryotic genomes. Chrysanthemum is one of the largest genera Asteraceae family. Only few expressed tag (EST) sequences have been acquired to date, so number available EST-SSR markers very low. Methodology/Principal Findings Illumina paired-end sequencing technology produced over 53 million reads from C. nankingense mRNA. The subsequent de novo assembly yielded 70,895 unigenes, which 45,789 (64.59%) unigenes showed similarity NCBI...
Flavones are among the major colorless pigments synthesized through branches of flavonoid pathway in plants. However, due to absence a gene encoding flavone synthase (FNS) model plant Arabidopsis thaliana species, regulatory mechanism FNS-catalyzed biosynthesis has rarely been studied Here, it was found that flavones play predominant role elimination excess reactive oxygen species (ROS) at high temperatures organs. A novel atypical subgroup 7 (SG7) R2R3-MYB transcription factor, CmMYB012, be...
ABSTRACT Light is one of the most important environmental factors that affect plant growth and development. It also stimulates anthocyanin biosynthesis in plants. However, precise molecular mechanisms through which light regulates biosynthesis, particularly non‐model species, remain poorly understood. In this study, we discovered a CmBBX28‐CmMYB9a module responsive to chrysanthemums. Specifically, CmBBX28 interacts with CmMYB9a, interfering its binding promoters target genes reducing protein...
SUMMARY Branching is the main factor that determines plant architecture and closely related to adaptation environment. Cold stress can inhibit lateral bud elongation in plants, but underlying mechanism still unclear. Here, we report cold stress‐induced bZIP family transcription CmbZIP19 inhibits chrysanthemum. We identified target gene of as brassinolide (BR) synthesis‐related CmDWF1 by integrating RNA‐seq DAP‐seq data. directly bind ZDRE‐like motif promoter region , thereby inhibiting...
Heat shock proteins are associated with protection against various abiotic stresses. Here, the isolation of a chrysanthemum cDNA belonging to HSP70 family is reported. The cDNA, designated CgHSP70, encodes 647-residue polypeptide, estimated molecular mass 70.90 kDa and pI 5.12. A sub-cellular localization assay indicated that product deposited in cytoplasm nucleus. performance Arabidopsis thaliana plants constitutively expressing CgHSP70 demonstrated gene enhances tolerance heat, drought...
Summary Salinity‐induced Na + toxicity and oxidative stress hamper plant growth. Here, we showed that expression of the chrysanthemum Cm HSFA 4 , a homologue heat‐shock factor At 4a is inducible by salt localizes to nucleus. It transcription activator binding with HSE . Chrysanthemum overexpressing displayed enhanced salinity tolerance limiting accumulation while maintaining K concentration, which consistent up‐regulation ion transporters SOS 1 HKT 2 Additionally, transgenic plants reduced H...
MYB transcription factors are widely involved in the development of and physiological processes plants. Here, we isolated chrysanthemum R2R3-MYB family factor CmMYB15, a homologous gene AtMYB15. It was demonstrated that CmMYB15 expression induced by aphids could bind to AC elements, which usually exist promoter lignin biosynthesis genes. Overexpression enhanced resistance aphids. Additionally, content several genes increased. In summary, results indicate regulates enhance
ICE (Inducer of CBF Expression) family genes play an important role in the regulation cold tolerance pathways. In earlier study, we isolated gene CdICE1 from Chrysanthemum dichrum and demonstrated that freezing was enhanced by overexpression. Therefore, sought to determine mechanism which ICE1 participate tolerance.Using EMSA (Electrophoretic Mobility Shift Assay) yeast one-hybrid assays, confirmed binds specifically MYC element CdDREBa promoter activates transcription. addition,...
WRKY transcription factors serve as antagonistic or synergistic regulators in a variety of abiotic stress responses plants. Here, we show that CmWRKY1, member the group IIb family isolated from Chrysanthemum morifolium, exhibits no transcriptional activation yeast cells. The subcellular localization examination showed CmWRKY1 localizes to nucleus vivo. Furthermore, CmWRKY1-overexpressing transgenic lines exhibit enhanced dehydration tolerance response polyethylene glycol (PEG) treatment...
The family of DNA binding with one finger (DOF) transcription factors is plant specific, and these proteins contain a highly conserved domain (DOF domain) 50-52 amino acids that includes C2C2-type zinc motif at the N-terminus known to function in number processes. Here, we characterized 20 DOF genes important ornamental species chrysanthemum (Chrysanthemum morifolium) based on transcriptomic sequences. Phylogenetic analysis identified pair putative orthologous Arabidopsis six pairs...
Drought is one of the important abiotic factors that adversely affects plant growth and production. The WRKY transcription factor plays a pivotal role in development, as well elevation many stresses. Among three major groups family, group IIe has been least studied floral crops. Here, we report functional aspects member, i.e., CmWRKY10 chrysanthemum involved drought tolerance. transactivation assay showed had transcriptional activity yeast cells subcellular localization demonstrated it was...
Waterlogging stress is among the most severe abiotic stressors in breeding and production of Chrysanthemum morifolium. However, mechanism underlying response to waterlogging post-waterlogging reoxygenation C. morifolium remains unknown. In this study, we compared differences between transcriptomes two chrysanthemum cultivars, i.e., waterlogging-tolerant cultivar “Nannongxuefeng” waterlogging-sensitive “Qinglu”, by performing RNA-seq elucidate possible had a higher ethylene under conditions....
Sustained monoculture often leads to a decline in soil quality, particular the build-up of pathogen populations, problem that is conventionally addressed by use either fungicide and/or fumigation. This practice no longer considered be environmentally sustainable or safe. While application organic fertilizer seen as means combating declining fertility, it has also been suggested providing some control over certain soil-borne plant pathogens. Here, greenhouse comparison was made Fusarium wilt...
Abstract Chrysanthemum (Chrysanthemum morifolium) is one of the four major cut-flower plants worldwide and possesses both high ornamental value cultural connotation. As most chrysanthemum varieties flower in autumn, it costly to achieve annual production. JAZ genes TIFY family are core components jasmonic acid (JA) signaling pathway; addition playing a pivotal role plant responses defense, they also widely implicated regulating development processes. Here, we characterized gene CmJAZ1-like...
Abstract The timely transition from vegetative to reproductive development is coordinated through the quantitative regulation of floral pathway genes in response physiological and environmental cues. function ethylene‐responsive element‐binding protein (ERF) transcription factors flowering chrysanthemum ( Chrysanthemum morifolium Ramat.) not well understood. Here, overexpressing CmERF110 flowered earlier than wild‐type plants, while those which was suppressed later. RNA‐seq results revealed...