- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Light effects on plants
- Photoreceptor and optogenetics research
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant-Microbe Interactions and Immunity
- Polymer-Based Agricultural Enhancements
- Plant tissue culture and regeneration
- Antioxidant Activity and Oxidative Stress
- Marine and coastal ecosystems
- Plant nutrient uptake and metabolism
- Growth and nutrition in plants
- Chromosomal and Genetic Variations
- Plant responses to water stress
- Seedling growth and survival studies
- Legume Nitrogen Fixing Symbiosis
- Potato Plant Research
- Wheat and Barley Genetics and Pathology
- Plant Pathogens and Resistance
- Plant Micronutrient Interactions and Effects
- Plant and animal studies
- Plant Virus Research Studies
- Seed Germination and Physiology
- Plant responses to elevated CO2
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2022
University of Buenos Aires
2010-2022
Experimental Medicine and Biology Institute
2018-2022
Fundación Ciencias Exactas y Naturales
2010-2022
Instituto de Biodiversidad y Biología Experimental y Aplicada
2020
National Agricultural Technology Institute
2016
Centro Científico Tecnológico - San Juan
2016
Instituto de Genética Veterinaria
2012
University of California, Berkeley
1997-2009
Australian National University
2004-2007
Xanthophylls participate in light harvesting and are essential protecting the chloroplast from photooxidative damage. To investigate roles of xanthophylls photoprotection, we isolated characterized extragenic suppressors npq1 lor1 double mutant Chlamydomonas reinhardtii, which lacks zeaxanthin lutein undergoes irreversible bleaching cell death at moderate to high intensities. Here, describe three suppressor strains that carry point mutations coding sequence epoxidase gene, resulting...
Abstract To determine the contribution of photosynthesis on stomatal conductance, we contrasted red light response wild-type tobacco (Nicotiana tabacum ‘W38’) with that plants impaired in by antisense reductions content either cytochrome b 6 f complex (anti-b/f plants) or Rubisco (anti-SSU plants). Both transgenic genotypes showed a lowered target proteins guard cells as well mesophyll. In anti-b/f plants, CO2 assimilation rates were proportional to leaf content, but there was little effect...
When there is an imbalance between the light energy absorbed by a photosynthetic organism and that which can be utilized in photosynthesis, photo-oxidative stress damage pigments, proteins, lipids, nucleic acids. In this work we compared wild type xanthophyll-deficient mutant of Chlamydomonas reinhardtii their response to high amounts light. Wild-type cells were able acclimate following transfer from low conditions. contrast, npq1 lor1 double mutant, lacks protective xanthophylls (zeaxanthin...
Abstract Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) plays a key role during C4 photosynthesis and is involved in anaplerotic metabolism, pH regulation, stomatal opening. Heterozygous (Pp) homozygous (pp) forms of PEPC-deficient mutant the dicot Amaranthus edulis were used to study effect reduced PEPC activity on CO2 assimilation rates, conductance, 13CO2 (Δ13C) C18OO (Δ18O) isotope discrimination leaf gas exchange. was 42% 3% rates air dropped 78% 10% wild-type values Pp pp mutants,...
Abstract Eucalyptus grandis is a globally important tree crop. Greenhouse-grown seedlings often face water deficit after outplanting to the field, which can affect their survival and establishment severely. This be alleviated by application of superabsorbent hydrophilic polymers (SAPs). Growth promoting bacteria also improve crop abiotic stress tolerance; however, use in trees limited, partly due difficulties viability loss. In this work, we evaluated improvement drought tolerance E....
Compatible plant viral infections are a common cause of agricultural losses worldwide. Characterization the physiological responses controlling water management under combined stresses is great interest in current climate change scenario. We studied outcome TuMV infection on stomatal closure and balance, hormonal balance drought tolerance Arabidopsis. reduced aperture concomitantly with diminished gas exchange rate, daily consumption rosette initial dehydration rate. Infected plants...
Abstract Sucrose metabolism is important for most plants, both as the main source of carbon and via signaling mechanisms that have been proposed this molecule. A cleaving enzyme, invertase (INV) channels sucrose into sink metabolism. Although acid soluble insoluble invertases largely investigated, studies on role neutral (A/N-INV) lagged behind. Here, we identified a tomato A/N-INV encoding gene (NI6) co-localizing with previously reported quantitative trait locus (QTL) affecting primary in...
Abstract We have investigated the electron transfer kinetics for reduction of plastoquinone by photo system II in six mutant strains Chlamydomonas reinhardtii following decay high fluorescence state after flash activation, and compared separate reactions two-electron gate with those a wild type strain. By analysis kinetics, measurement equilibrium constant stabilization bound semiquinone one flash, we been able to deconvolute contributions rate constants binding overall process. Two...
ABSTRACT Tocopherols (vitamin E) comprise a class of lipid-soluble antioxidants synthesized only in plants, algae, and some cyanobacteria. The majority tocopherols photosynthetic cells is the α form, which has highest vitamin E activity humans, whereas β, γ, δ forms normally account for small percentage total tocopherols. antioxidant activities these tocopherol differ depending on experimental system, their relative vivo are unclear. In screen suppressors xanthophyll-deficient npq1 lor1...
In this study, agronomic and functional characteristics of potato (Solanum tuberosum ) plants constitutively overexpressing the protein phosphatase 2A (PP2A) catalytic subunit StPP2Ac2b (StPP2Ac2b-OE) were evaluated. StPP2Ac2b-OE display reduced vegetative growth, tuber yield weight under well-watered drought conditions. Leaves show an increased rate water loss, associated with impaired ability to close stomata in response abscisic acid. lines exhibit larger stomatal size density. These...
i) Summary In this work the effects of TuMV infection on stomatal closure and water balance were studied in Arabidopsis. Thermal imaging analyses showed that TuMV-infected plants had consistently higher foliar temperature than mock treated controls. Non-destructive time-course experiments revealed differential phenotype was stable during both daytime nighttime. This effect due to reduced gas exchange plants, as observed through conductance aperture assays systemic leaves. Measurements daily...
Algae and plants have evolved numerous mechanisms to cope with the potential for photo-oxidative damage that accompanies oxygenic photosynthesis. Photosynthetic light harvesting is regulated by processes such as nonphotochemical quenching (NPQ) help minimize production of reactive oxygen species, various antioxidant molecules enzymes are involved in scavenging or inevitably generated. NPQ antioxidants being investigated using mutants Arabidopsis thaliana Chlamydomonas reinhardtii....
Xanthophylls are essential in protecting the chloroplast from photooxidative damage. The zeaxanthin- and lutein-deficient double mutant of Chlamydomonas reinhardtii, npq1 lor1, shows negligible energy-dependent nonphotochemical quenching chlorophyll fluorescence undergoes irreversible bleaching at moderate light intensities. When transferred low to light, cellular content decreases undetectable levels, parallel with an increase lipid peroxides disappearance thylakoid membranes. We have...