A5070372870- Probiotics and Fermented Foods
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Catalysis and Immobilization
- Proteins in Food Systems
- Enzyme Production and Characterization
- Biotin and Related Studies
- biodegradable polymer synthesis and properties
- Biochemical and biochemical processes
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Microplastics and Plastic Pollution
- Digestive system and related health
- Meat and Animal Product Quality
- Protein purification and stability
- Additive Manufacturing and 3D Printing Technologies
- Biochemical Acid Research Studies
Novozymes (Denmark)
2023-2024
Technical University of Denmark
2017-2021
Ecologie Microbienne Lyon
2020
KTH Royal Institute of Technology
2017
AlbaNova
2017
Ruhr University Bochum
2014
Bioprocessing of poly(ethylene terephthalate) (PET) waste has emerged as a promising approach to circular economy. The key this development been the discovery enzymes that effectively depolymerize PET its constituent monomers, and hitherto, most candidates are quite closely related, bacterial cutinases. Fungal have less investigated, although few examples hydrolytic activity against reported. To further assess this, we produced 24 fungal cutinases homologues known hydrolases identified three...
Lactococcus lactis subsp. biovar diacetylactis is widely used in dairy fermentations as it can form the butter aroma compounds acetoin and diacetyl from citrate milk. Here, we explore possibility of producing more abundant lactose. Starting a isolate L. diacetylactis, obtained series mutants with low lactate dehydrogenase (ldh) activity. One isolate, RD1M5, only had single insertion mutation ldh gene compared to its parental strain revealed by whole genome resequencing. We tested ability...
In the manufacture of rennet-coagulated cheese, autolysis is a rate-limiting step for ripening. Previously, highly autolytic and thermotolerant Lactococcus lactis strain, RD07, was generated, which in preliminary laboratory cheese trials demonstrated great potential as ripening accelerant. RD07 proteinase positive (Prt+) capable metabolizing citrate (Cit+). this study, we obtained two derivatives RD07: EC8 lacking plasmid, EC2 plasmid. retained properties autolyzed 20 times faster than Flora...
Nisin is commonly used as a biopreservative in foods. For industrial production, nisin-producing Lactococcus lactis strains are usually grown to high cell densities achieve the highest possible nisin titer. However, accumulation of lactic acid eventually halts even pH-controlled fermentations. Here, we describe L. strain Ge001, which was obtained after transferring gene cluster from ATCC 11454, by conjugation, into natural mutant RD1M5, with low lactate dehydrogenase activity. The ability...
In commercial mesophilic starter cultures, L. lactis is generally more thermotolerant than Lactococcus cremoris , whereas prone to autolysis, which the key flavor and aroma formation. this study, we found that adaptation higher thermotolerance can improve autolysis.
Abstract Background Diacetyl provides the buttery aroma in products such as butter and margarine. It can be made via a harsh set of chemical reactions from sugarcane bagasse, however, dairy it is normally formed spontaneously α-acetolactate, compound generated by selected lactic acid bacteria starter culture used. Due to its bacteriostatic properties, difficult achieve high levels diacetyl fermentation. Here we present novel strategy for producing based on whole-cell catalysis, which...
The genome of Lactococcuslactis subsp. lactis bv. diacetylactis SD96, a strain used for cheese production, is presented. SD96 refractory to phage attack, which desired property starter bacteria. Its 10 plasmids provide industrially important traits, such as lactose and citrate metabolism, proteolytic activity, resistance.
Generally, the catalytic activity of subtilisin Carlsberg (SC) for transacylation reactions with secondary alcohols in organic solvent is low. Enzyme immobilization and protein engineering was performed to improve enantioselectivity SC towards alcohols. Possible amino-acid residues mutagenesis were found by combining available literature data molecular modeling. variants created site-directed evaluated a model reaction containing 1-phenylethanol THF. Variants showing high E values (>100)...