A5056723892- CRISPR and Genetic Engineering
- Viral Infections and Immunology Research
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- Hepatitis B Virus Studies
- HIV Research and Treatment
- RNA Interference and Gene Delivery
The University of Melbourne
2021-2023
Peter Doherty Institute
2021-2022
The recent dramatic appearance of variants concern SARS-coronavirus-2 (SARS-CoV-2) highlights the need for innovative approaches that simultaneously suppress viral replication and circumvent escape from host immunity antiviral therapeutics. Here, we employ genome-wide computational prediction single-nucleotide resolution screening to reprogram CRISPR-Cas13b against SARS-CoV-2 genomic subgenomic RNAs. Reprogrammed Cas13b effectors targeting accessible regions Spike Nucleocapsid transcripts...
Background: People with HIV-HBV co-infection have faster rates of liver disease progression and an increase in hepatocellular carcinoma compared to people HBV mono-infection. Given that HIV can infect multiple cells the including hepatocytes, we hypothesized direct interactions between hepatocyte, presence absence HBV-active ART, drives adverse outcomes (HCC).Methods: The hepatocyte cell line HepG2 expressing sodium taurocholate co-transporting polypeptide (NTCP) was infected inoculum...