A5073931664- Gut microbiota and health
- Parasitic Infections and Diagnostics
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Insects and Parasite Interactions
- Bacteriophages and microbial interactions
- Oral microbiology and periodontitis research
- Dermatological diseases and infestations
- Gene expression and cancer classification
- Respiratory viral infections research
- Salivary Gland Disorders and Functions
- Text and Document Classification Technologies
- Parasitic infections in humans and animals
- Probiotics and Fermented Foods
- Travel-related health issues
- Toxoplasma gondii Research Studies
- Amoebic Infections and Treatments
- Bioinformatics and Genomic Networks
- Birth, Development, and Health
- Clostridium difficile and Clostridium perfringens research
- Congenital Anomalies and Fetal Surgery
- Oral Health Pathology and Treatment
- IL-33, ST2, and ILC Pathways
- Protist diversity and phylogeny
- Dental Health and Care Utilization
Copenhagen Prospective Studies on Asthma in Childhood
2023-2024
University of Copenhagen
2015-2024
Gentofte Hospital
2023-2024
Novo Nordisk Foundation
2022-2024
Rigshospitalet
2023
Aalborg University Hospital
2023
Technical University of Denmark
2019-2022
Aarhus University
2022
Novo Nordisk Foundation Center for Basic Metabolic Research
2022
Food Research Institute
2019
Abstract Background Multiple sclerosis is a chronic immune-mediated disease of the brain and spinal cord resulting in physical cognitive impairment young adults. It hypothesized that disrupted bacterial viral gut microbiota part pathogenesis mediating impact through an altered microbiota-brain axis. The aim this study to explore characteristics multiple associate it with variables, as etiology remains only partially known. Methods Here, case-control setting involving 148 Danish cases matched...
Previous research has reported effects of DNA isolation, library preparation, and sequencing technology on metagenomics-based microbiome composition; however, the effect biospecimen storage conditions not been thoroughly assessed. We examined common sample composition found significant and, in part, systematic effects.
This study aimed to elucidate aspects of the epidemiology Blastocystis in Nigerian school children, including distribution subtypes (STs) and ST alleles. A total 199 genomic DNAs extracted from fecal samples children aged 2–14 years were tested by real-time polymerase chain reaction for . Positive submitted barcoding PCR sequencing obtain information on STs 167 (84%) positive , with prevalence increasing age. No association between colonization gender ( P = 0.51) or type/presence toilet...
One of the most common zoonotic helminth infections is caused by species in genus Toxocara, particularly Toxocara canis and T. cati (Syn. mystax). However, their relative contribution to toxocarosis humans remains largely unknown because causative larvae are seldom recovered uncertainties regarding validity existing serological assays. In this study, we used sera from a pig model experimentally infected with evaluate whether Western blot could discriminate between two species. No proteins...
Culture techniques have associated colonization with pathogenic bacteria in the airways of neonates later risk childhood asthma, whereas more recent studies utilizing sequencing shown same phenomenon specific anaerobic taxa. Here, we analyze nasopharyngeal swabs from 1 month COPSAC2000 prospective birth cohort by 16S rRNA gene V3-V4 region relation to asthma throughout childhood. Results are compared previous culture results hypopharyngeal aspirates and data COPSAC2010 cohort. Nasopharyngeal...
One Health surveillance of antimicrobial resistance (AMR) depends on a harmonized method for detection AMR. Metagenomics-based offers the possibility to compare resistomes within and between different target populations. Its potential be embedded into policy in future calls timely integrated knowledge dissemination strategy. We developed blended training (e-learning workshop) use metagenomics pathogens The objectives were highlight context surveillance, demonstrate its applicability through...
Previous research indicates that the salivary microbiota may be a biomarker of oral as well systemic disease. However, clarifying potential bias from general health status and lifestyle-associated factors is prerequisite using for screening.ADDDITION-PRO nationwide Danish cohort, nested within arm Anglo-Danish-Dutch Study Intensive treatment in People with Screen-Detected Diabetes Primary Care. Saliva samples n=746 individuals ADDITION-PRO cohort were characterized 16s rRNA sequencing....
Abstract Background Over a billion people are infected with Toxocara canis or T. cati , the roundworms of dogs and cats. Historically, has been considered main species responsible for human toxocarosis, but as serodiagnosis cannot discriminate between two species, this remains unresolved. We used pigs relevant large animal model infection to assess migratory pattern . Methods Pigs were inoculated eggs PBS (negative controls) necropsied 14 31 days later. Different organs tissues examined...
Abstract The microbial communities of the oral cavity are important elements and systemic health. With emerging evidence highlighting heritability bacterial microbiota, this study aimed to identify host genome variants that influence traits. Using data from 16S rRNA gene amplicon sequencing, we performed genome-wide association studies with univariate multivariate traits salivary microbiota 610 unrelated adults Danish ADDITION-PRO cohort. We identified six single nucleotide polymorphisms...
<title>Abstract</title> The <italic>Anelloviridae</italic> family is highly prevalent and genetically diverse, but factors shaping the composition relationship with disease remain elusive. Here, we characterised faecal viromes of 647 one-year-old children from deeply phenotyped Copenhagen Prospective Studies on Asthma in Childhood<sub>2010</sub> (COPSAC<sub>2010</sub>) mother-child cohort. In this study, anelloviruses showed immense prevalence individual variability. Early-life exposures...
Abstract Metagenomics is increasingly used to describe microbial communities in biological specimens. Ideally, the steps involved processing of specimens should not change microbiome composition a way that it could lead false interpretations inferred community composition. Common sample preparation include collection, storage, DNA isolation, library preparation, and sequencing. Here we assess effect three kits two sequencing platforms. Of kits, one polymerase chain reaction (PCR) step...
An inter-laboratory proficiency test was organized to assess the ability of participants perform shotgun metagenomic sequencing cold smoked salmon, experimentally spiked with a mock community composed six bacteria, one parasite, yeast, DNA, and two RNA viruses. Each participant applied its in-house wet-lab workflow(s) obtain dataset(s), which were then collected analyzed using MG-RAST. A total 27 datasets analyzed. Sample pre-processing, DNA extraction protocol, library preparation kit,...
Abstract Storage of biological specimens is crucial in the life and medical sciences. The storage conditions for samples can be different a number reasons, it unclear which effect this have on inferred microbiome composition metagenomics analyses. Here, we assess common temperatures (deep freezer: -80°C, -20°C, fridge: 5°C, room temperature: 22°C) times, (immediate sample processing: 0h, next day: 16h, over weekend: 64h, longer term: 4, 8, 12 months), as well repeated freezing thawing (2-4...
Latent autoimmune diabetes in adults (LADA) is a heterogeneous disease characterized by autoantibodies against insulin producing pancreatic beta cells and initial lack of need for treatment. The aim the present study was to investigate if individuals with LADA have an altered gut microbiota relative non-diabetic control subjects, type 1 (T1D), 2 (T2D). Bacterial community profiling performed primers targeting variable region 4 16S rRNA gene sequenced. Amplicon sequence variants (ASVs) were...
Abstract Over a billion people are infected with Toxocara canis or T. cati , the roundworms of dogs and cats. Historically, has been considered main responsible human toxocarosis but as serodiagnosis cannot discriminate two species, this remains unresolved. We assessed migratory pattern in pig model found them to be equally infective. Overall, they had similar migration reaching multiple organs tissues, including mesenteric lymph nodes, liver, lungs diaphragm. recovered larvae both species...
Sebaceous hyperplasia is a benign, not uncommon cutaneous disorder. The disorder caused by overabundance and proliferation of sebocytes in the sebaceous glands [1]. induces enlargement gland up to several times its regular size, but still keeping normal anatomical structures. Sebacceous have central duct that may become dilated due secretion an oily substance, sebum, which presents flesh- or yellowish coloured [2]. are localized everywhere on skin except for palms soles feet. highest...
Abstract Microbial metagenomics utilising next generation sequencing is a powerful experimental approach enabling detailed and potentially complete descriptions of the microbial world around within us. Selecting how to perform feature data normalization, transformation calculate ß-diversity critical step in analysis metagenomic data, but also for which multitude methods are available. Researchers need have broad overview understand many that exist field consequences from applying them. In...