A5003741192- Gut microbiota and health
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Colorectal Cancer Screening and Detection
- Rheumatoid Arthritis Research and Therapies
- SARS-CoV-2 detection and testing
- Mycobacterium research and diagnosis
- interferon and immune responses
- Cancer-related molecular mechanisms research
- Advanced Biosensing Techniques and Applications
- Microbial Community Ecology and Physiology
- Cytokine Signaling Pathways and Interactions
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
Foundation for Research and Technology Hellas
2020-2022
Hellenic Centre for Marine Research
2020-2021
University of Crete
2019-2020
16S rRNA gene amplicon sequencing is a popular approach for studying microbiomes. However, some basic concepts have still not been investigated comprehensively. We studied the occurrence of spurious sequences using defined microbial communities based on data either from literature or generated in three facilities and analyzed via both operational taxonomic units (OTUs) sequence variants (ASVs) approaches. OTU clustering singleton removal, commonly used approach, delivered approximately 50%...
Loop-mediated isothermal amplification is known for its high sensitivity, specificity and tolerance to inhibiting-substances. In this work, we developed a device performing real-time colorimetric LAMP combining the accuracy of lab-based quantitative analysis with simplicity point-of-care testing. The innovation lies on use plastic tube anchored vertically hot surface while side walls are exposed mini camera able take snapshots colour change in real time during amplification. Competitive...
Anti-TNF agents have been in the first line of treatment various inflammatory diseases such as Rheumatoid Arthritis and Crohn's Disease, with a number different biologics being currently use. A detailed analysis their effect at transcriptome level has nevertheless lacking. We herein present concise an extended transcriptomics profiling four anti-TNF upon established hTNFTg (Tg197) mouse model spontaneous polyarthritis. implement series computational analyses that include clustering...
Abstract Background: 16S rRNA gene amplicon sequencing is a very popular approach for studying microbiomes. However, varying standards exist sample and data processing some basic concepts such as the occurrence of spurious sequences have not been investigated in comprehensive manner, which was done present study. Methods: Using defined communities bacteria vitro vivo , we searched matching expected species ( i.e. taxa) determine threshold relevant adequate analysis. The origin taxa then via...
Abstract Loop-mediated isothermal amplification is known for its high sensitivity, specificity and tolerance to inhibiting-substances. We developed a device performing real-time colorimetric LAMP combining the accuracy of lab-based quantitative molecular diagnosis with simplicity point-of-care testing. This handheld employs single reaction-pot mini-camera detection. Competitive features are rapid analysis (<30min), quantification over 9 log-units, crude sample-compatibility (saliva,...
Abstract Background: 16S rRNA gene amplicon sequencing is a very popular approach for studying microbiomes. However, varying standards exist sample and data processing some basic concepts, such as the occurrence of spurious sequences, have not been investigated in comprehensive manner. Methods: Using defined communities bacteria vitro vivo , we searched sequences matching expected species ( i.e. taxa) determined minimum threshold suitable robust analysis. The presence origin taxa were via...