A5059211017- Cellular Mechanics and Interactions
- Collagen: Extraction and Characterization
- Skin and Cellular Biology Research
- Silk-based biomaterials and applications
- Tendon Structure and Treatment
- Force Microscopy Techniques and Applications
- Connective tissue disorders research
- Hair Growth and Disorders
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Cardiomyopathy and Myosin Studies
- Dyeing and Modifying Textile Fibers
- Elasticity and Material Modeling
- Bone Tissue Engineering Materials
- Advanced Electron Microscopy Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced biosensing and bioanalysis techniques
- Muscle Physiology and Disorders
- Advanced Materials and Mechanics
- Polymer Surface Interaction Studies
- Nuclear Structure and Function
- Supramolecular Self-Assembly in Materials
- Gear and Bearing Dynamics Analysis
- Protease and Inhibitor Mechanisms
- Advanced Fluorescence Microscopy Techniques
Dalhousie University
2015-2024
Université du Québec à Trois-Rivières
2010
University of Basel
2003-2009
Université du Québec
2008-2009
Laboratoire de Physique du Rayonnement et de la Lumière
2001-2002
Université Paris-Sud
2001
Intermediate filaments (IFs), in addition to microtubules and microfilaments, are one of the three major components cytoskeleton eukaryotic cells, playing a vital role mechanotransduction providing mechanical stability cells. Despite importance IF mechanics for cell biology mechanics, structural basis their properties remains unknown. Specifically, our understanding fundamental filament properties, such as great extensibility, stiffening exceptional resilience limited. This has prevented us...
Complexes of cationic liposomes with DNA are promising tools to deliver genetic information into cells for gene therapy and vaccines. Electrostatic interaction is thought be the major force in lipid–DNA interaction, while lipid-base binding stability complexes have been subject more debate recent years. The aim this study was examine complexation calf-thymus cholesterol (Chol), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dioctadecyldimethylammoniumbromide (DDAB)...
Dendrimers are unique synthetic macromolecules of nanometer dimensions with a highly branched structure and globular shape. Among dendrimers, polyamidoamine (PAMAM) have received most attention as potential transfection agents for gene delivery, because these bind DNA at physiological pH. The aim this study was to examine the interaction calf-thymus several dendrimers different compositions, such mPEG-PAMAM (G3), (G4), PAMAM (G4) conditions, using constant concentration various dendrimer...
Intermediate filaments (IFs), along with microtubules, microfilaments, and associated cross-bridging proteins, constitute the cytoskeleton of metazoan cells. While crystallographic data on dimer representing elementary IF "building block" have recently become available, little structural detail is known about both mature architecture its assembly pathway. Here, we applied solution small-angle x-ray scattering to investigate in vitro a 53-kDa human protein vimentin at pH 8.4 by systematically...
Oxysterol-binding protein (OSBP) and OSBP-related proteins (ORPs) comprise a large gene family with sterol/lipid transport regulatory activities. ORP4 (OSBP2) is closely related paralogue of OSBP, but its function unknown. Here we show that binds similar sterol lipid ligands as OSBP other ORPs uniquely required for the proliferation survival cultured cells. Recombinant ORP4L variant without pleckstrin homology (PH) domain (ORP4S) bind 25-hydroxycholesterol extract transfer cholesterol...
To determine the effects of tooth brushing on five bulk-fill resin based composites (RBCs).Ten samples Filtek Supreme Enamel (control), One Bulk Fill, Tetric EvoCeram SonicFill 2, SDR flow+, and Admira Fusion X-tra were light cured for 20 seconds using Valo Grand curing light. After 24 hours storage in air at 37°C, specimens brushed a random order Colgate OpticWhite dentifrice soft toothbrush. Surface gloss was measured prior to brushing, after 5,000, 10,000 15,000 back forth cycles....
The collagen-based tissues of animals are hierarchical structures: even tendon, the simplest collagenous tissue, has seven to eight levels hierarchy. Tailoring tissue structure match physiological function can occur at many different levels. We wanted know if control architecture achieve extends down nanoscale level individual, cable-like collagen fibrils. Using tendons from young adult bovine forelimbs, we performed stress-strain experiments on single fibrils extracted with positional...
Myofibril elasticity, critical to muscle function, is dictated by the intrasarcomeric filament titin, which acts as a molecular spring. To date, events underlying mechanics of folded titin chain remain largely unknown. We have elucidated crystal structure 6-Ig fragment I65–I70 from elastic I-band fraction and validated its conformation in solution using small angle x-ray scattering. The long-range properties been visualized electron microscopy on 19-Ig modeled for full skeletal tandem....
Second harmonic generation (SHG) microscopy is a commonly used technique to study the organization of collagen within tissues. However, individual fibrils, which have diameters much smaller than resolution most optical systems, not been extensively investigated. Here we probe structure fibrils using polarization-resolved SHG (PSHG) and atomic force microscopy. We find that longitudinally polarized light occurring at edge focal volume high numerical aperture microscope objective illuminated...
Despite considerable interest and investigations on cationic lipid-DNA complexes, reports lipid-RNA interaction are very limited. In contrast to complexes where lipid binding induces partial B A C conformational changes, lipid-tRNA complexation preserves tRNA folded state. This study is the first attempt investigate of with transfer RNA effect aggregation condensation. We examine cholesterol (Chol), 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), dioctadecyldimethylammoniumbromide (DDAB)...
The cell's cytoskeleton, providing the cell with structure and shape, consists of a complex array structural proteins, including microtubules, microfilaments intermediate filaments. Intermediate filaments play crucial role in mechanotransduction mechanical stability to cells, particular under large deformation. By utilizing molecular simulation, here we report nanomechanical analysis vimentin filament dimers, basic building blocks We describe detailed properties associated deformation...
Excellent device characteristics are measured for a pentacene-based thin film transistor where the SiO2 gate dielectric is terminated with self-assembled monolayer of 9,10-dinaphthylanthracene-2-phosphonate in which calculated molecular spacings about 0.7 nm. This creates channels that on order "thickness" an aromatic π system, could allow intercalation pentacene units, favoring π-stacking motif this first layer. Detailed facts importance to specialist readers published as "Supporting...