A5048469142- Lipid Membrane Structure and Behavior
- Spectroscopy and Quantum Chemical Studies
- Renal function and acid-base balance
- Analytical Chemistry and Chromatography
- Protein Interaction Studies and Fluorescence Analysis
- Antibiotics Pharmacokinetics and Efficacy
- Photosynthetic Processes and Mechanisms
- Advancements in Transdermal Drug Delivery
- Drug Transport and Resistance Mechanisms
- Axial and Atropisomeric Chirality Synthesis
- Sphingolipid Metabolism and Signaling
- Radioactive element chemistry and processing
- Surfactants and Colloidal Systems
Institut des Sciences Moléculaires
2025
University of Coimbra
2022-2025
Rifampicin is a broad-spectrum antibiotic, active against several bacterial infections such as tuberculosis. It relatively large and structurally complex molecule, including numerous polar groups. Although violating of Lipinski’s rules for efficient intestinal absorption, rifampicin permeates through cell membranes its target organisms shows good oral bioavailability. Some hypotheses have been proposed membrane permeation, but the details are mostly unknown. In this work, interaction with...
Bioavailability of a drug is critically dependent on its cell membrane permeability. Empirical rules guiding design consolidated the dogma that large molecules cannot cross membranes by passive diffusion. However, more amphiphilic atropisomers redaporfin, an 1135 Da bacteriochlorin photosensitizer used in photodynamic therapy, exhibited fast uptake and high activity vitro. This motivated detailed studies redaporfin their interactions with models. Experimental affinity, permeation rates,...
Rifampicin is a broad-spectrum antibiotic, active against several bacterial infections such as tuberculosis. It relatively large and structurally complex molecule, including numerous polar groups. Although violating of Lipinski’s rules for efficient intestinal absorption, rifampicin shows good oral bioavailability, permeating through cell membranes in the absorption pathway those target organisms. Some hypotheses have been proposed its membrane permeation, but details are mostly unknown. In...
Hoechst 33342 (H33342) is a fluorescent probe that commonly used to stain the DNA of living cells. To do so, it needs interact with and permeate through cell membranes, despite its high overall charge at physiological pH values. In this work, we address effect in association H33342 lipid bilayers using combined experimental computational approach. The partition 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes was experimentally quantified fluorescence spectroscopy isothermal...
Hoechst 33342 (H33342) is a fluorescent probe that commonly used to stain DNA of living cells. To do so, it needs interact with and permeate through cell membranes. The pH biological media may vary quite significantly this influences H33342 ionization state, change its rate permeation biomembranes. In work, we address the effect in association lipid bilayers using combined experimental computational approach. partition 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes was...
Predicting the rate at which substances permeate membrane barriers in vivo is crucial for drug development. Permeability coefficients obtained from vitro studies are valuable this goal. These normally determined by following dynamics of solute equilibration between two membrane-separated compartments. However, correct calculation permeability such data not always straightforward. To address these problems, here we develop a kinetic model permeation through lipid that includes leaflets as...