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A5039006302
Research Areas
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
Max Planck Institute of Molecular Physiology
2020
Bacterial artificial chromosome (BAC)–based transgenes have emerged as a powerful tool for controlled and conditional interrogation of protein function in higher eukaryotes. Although homologous recombination-based recombineering methods streamlined the efficient integration tags onto BAC transgenes, generating precise point mutations has remained less time-consuming. Here, we present simplified method inserting into requiring single step followed by antibiotic selection. This technique,...
10.26508/lsa.202000836
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Life Science Alliance
2020-12-08
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