A5072122806- Cancer, Hypoxia, and Metabolism
- Advanced Breast Cancer Therapies
- Fibroblast Growth Factor Research
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Gene Regulatory Network Analysis
- Neuroblastoma Research and Treatments
- Innovative concrete reinforcement materials
- Growth Hormone and Insulin-like Growth Factors
- Cell Image Analysis Techniques
- Cancer Treatment and Pharmacology
- Melanoma and MAPK Pathways
- Adipose Tissue and Metabolism
- Cancer Cells and Metastasis
- Microbial Metabolic Engineering and Bioproduction
- Bioinformatics and Genomic Networks
- S100 Proteins and Annexins
- Hormonal Regulation and Hypertension
- Cancer, Stress, Anesthesia, and Immune Response
- bioluminescence and chemiluminescence research
- Histone Deacetylase Inhibitors Research
- Structural Behavior of Reinforced Concrete
- Adrenal and Paraganglionic Tumors
- Animal Genetics and Reproduction
- Computational Drug Discovery Methods
Instituto Butantan
2013-2024
The Netherlands Cancer Institute
2024
Universidade de São Paulo
2011-2023
Universidade Estadual Paulista (Unesp)
2021
Universidade Brasil
2000-2006
Universidade Federal de São Paulo
2003
University of British Columbia
1998
University of Toronto
1997
Associação Paulista de Medicina
1982
University of California, San Diego
1973
Results are reported showing that pituitary extracts stimulate growth of 3T3 cells, an established line mouse fibroblast. Cells were cultured in medium containing organic buffer (HEPES; N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), which increases the serum requirement for cell growth. The growth-prompting activity is (a) not dialyzable; (b) thermolabile; (c) protease sensitive; and (d) enhanced by hydrocortisone (a steroid hormone). Activity was quantitatively assayed measurement...
Abstract Cancer homeostasis depends on a balance between activated oncogenic pathways driving tumorigenesis and engagement of stress response programs that counteract the inherent toxicity such aberrant signaling. Although inhibition signaling has been explored extensively, there is increasing evidence overactivation same can also disrupt cancer cause lethality. We show here protein phosphatase 2A (PP2A) hyperactivates multiple engages responses in colon cells. Genetic compound screens...
The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels observed during the treatment with acids. dependent addition β-NADH or NADPH to medium. Diphenyleneiodonium inhibited fibroblast release 79%, 92% 82%, respectively. Increased p47phox phosphorylation due fatty acid were detected Western blotting analyses proteins. mRNA...
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The adrenocorticotropic hormone (ACTH) inhibits the growth of Y1 mouse adrenocortical tumor cells as well normal in culture but stimulates cell vivo. In this study, we investigated paradoxical effect ACTH on proliferation adrenal and have unmasked a growth-promoting hormone. were arrested G1 phase cycle by serum starvation monitored for progression through S measuring [3H]thymidine incorporation into DNA number nuclei labeled with bromodeoxyuridine. stimulated to progress divide after brief...
From the
Abstract Trypanosomatids are the etiologic agents of various infectious diseases in humans. They diverged early during eukaryotic evolution and have attracted attention as peculiar models for evolutionary comparative studies. Here, we show a meticulous study comparing incorporation detection thymidine analogs BrdU EdU Leishmania amazonensis , Trypanosoma brucei cruzi to monitor their DNA replication. We used BrdU‐ EdU‐incorporated parasites with respective standard approaches: indirect...
We recently reported that paracrine Fibroblast Growth Factor 2 (FGF2) triggers senescence in Ras-driven Y1 and 3T3Ras mouse malignant cell lines. Here, we show although FGF2 activates mitogenic pathways these Ras-dependent cells, it can block proliferation cause a G2/M arrest. These cytostatic effects of are inhibited by PD173074, an FGF receptor (FGFR) inhibitor. To determine which downstream induced FGF2, tested specific inhibitors targeting mitogen-activated protein kinase (MEK),...
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a classical metabolic enzyme involved in energy production and plays role additional nuclear functions, including transcriptional control, recognition of misincorporated nucleotides DNA maintenance telomere structure. Here, we show that the recombinant protein T. cruzi GAPDH (rTcGAPDH) binds single-stranded telomeric DNA. We demonstrate binding to correlates with balance between oxidized reduced forms nicotinamide adenine dinucleotides...
Summary Serum amyloid A (SAA) levels are elevated highly in acute phase response and slightly persistently chronic diseases such as rheumatoid arthritis diabetes. Given that fibroblasts exert profound effects on progression of inflammatory diseases, the aim this study was to investigate SAA. dose-dependent increase O2- observed by treatment with SAA (r = 0·99 P ≤ 0·001). In addition, expression p47-phox up-regulated (P < 0·001) diphenyliodonium (DPI), a nicotinamide adenine...
In malignant transformation, cellular stress‐response pathways are dynamically mobilized to counterbalance oncogenic activity, keeping cancer cells viable. Therapeutic disruption of this vulnerable homeostasis might change the outcome many human cancers, particularly those for which no effective therapy is available. Here, we report use fibroblast growth factor 2 ( FGF 2) demonstrate that further mitogenic activation disrupts and strongly sensitizes stress‐targeted therapeutic inhibitors. We...
We have shown that glucocorticoids reversibly change the growth control of rat C6 glioma cells from a transformed to normal pattern. Here we report glucocorticoid hormone hydrocortisone (Hy) modulates structure and function cell surface cytoskeleton. The is cause: (a) increased flattening adhesion solid substrates fibrin layers, (b) inhibition shape triggered by catecholamines cAMP, (c) extensive fibronectin deposition on normally fibronectinless cells' surface, (d) microtubule...
Abstract Fibroblast growth factor 2 (FGF2) is considered to be a bona fide oncogenic factor, although results from our group and others call this into question. Here, we report that exogenous recombinant FGF2 irreversibly inhibits proliferation by inducing senescence in Ras-dependent malignant mouse cells, but not immortalized nontumorigenic cell lines. We the following findings K-Ras–dependent Y1 adrenocortical cells H-Ras V12–transformed BALB-3T3 fibroblasts: (a) clonal tumor onset nude...
An ovarian cell line was developed that requires hormonal conditioning of the host for growth in vivo and special factors vitro. It is necessary to prepare culture media demonstrate effects To this end, methods were removing from serum those essential required cells culture. Minimal occurred medium containing fetal calf had been passed through a porcelain filter. This method depleting replaced by procedure involving passage carboxymethylcellulose column. Either pituitary extract or eluate...
Cells of the Y-1 corticoadrenal line are: (a) functional, (b) cell cycle-arrested by adrenocorticotropic hormone (ACTH), (c) tumorigenic, and (d) c-Ki-ras overexpressing. We here report that phorbol ester 12-myristate 13-acetate (PMA) mimics all ACTH-specific effects in cells, namely: steroid-ogenesis stimulation, cycle block, shape change. In addition, both ACTH PMA caused a rapid transient induction c-fos proto-oncogene while having no effect on mRNA steady state levels. Dibutyryl cAMP,...
ACTH is the hormone known to control adrenal cortex function and maintenance in intact animal but, culture, it inhibits proliferation of adrenocortical cells from different mammalian species, a puzzle that has remained unsolved for nearly 30 years. In this paper we compare fibroblast growth factor 2 (FGF2) antagonistic effects on cell cycle Y1 line, functional lineage mouse adreno-cortical tumor cells. This line displays chronic high levels c-Ki-Ras-GTP, active constitutive...