A5008534473- Hearing, Cochlea, Tinnitus, Genetics
- Hearing Loss and Rehabilitation
- Pluripotent Stem Cells Research
- Muscle Physiology and Disorders
- Lysosomal Storage Disorders Research
- Ear Surgery and Otitis Media
- Viral Infections and Immunology Research
- Renal and related cancers
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Animal Genetics and Reproduction
- Animal Virus Infections Studies
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- RNA regulation and disease
- Glycogen Storage Diseases and Myoclonus
- Immune Response and Inflammation
- Acoustic Wave Phenomena Research
- CRISPR and Genetic Engineering
- Noise Effects and Management
- Epigenetics and DNA Methylation
- Virus-based gene therapy research
DecImmune Therapeutics (United States)
2018-2024
Baxter (United States)
2007-2009
Stanford University
2005-2009
Mammalian hearing requires the development of organ Corti, a sensory epithelium comprising unique cell types. The limited number each these types, combined with their close proximity, has prevented characterization individual types and/or developmental progression. To examine cochlear more closely, we transcriptionally profile approximately 30,000 isolated mouse cells collected at four time points. Here report on analysis those including identification both known and unknown Trajectory for...
Noise-induced hearing loss (NIHL) results from a complex interplay of damage to the sensory cells inner ear, dysfunction its lateral wall, axonal retraction type 1C spiral ganglion neurons, and activation immune response. We use RiboTag single-cell RNA sequencing survey cell-type-specific molecular landscape mouse ear before after noise trauma. identify induction transcription factors STAT3 IRF7 immune-related genes across all cell-types. Yet, transcriptomic changes dominate The ATF3/ATF4...
DNA methylation is among the most stable epigenetic marks, ensuring tissue-specific gene expression in a heritable manner throughout development. Here we report that differentiated mesodermal somatic cells can confer changes on epidermal progenitor after fusion multinucleate heterokaryons. Myogenic factors alter regulatory regions of genes keratinocyte cell nuclei, demethylating and activating muscle-specific methylating silencing keratinocyte-specific gene. Because these occur absence...
An understanding of nuclear reprogramming is fundamental to the use cells in regenerative medicine. Due technological obstacles, time course and extent following fusion has not been assessed date. Here, we show that hundreds genes are activated or repressed within hours human keratinocytes mouse muscle heterokaryons, extensive changes observed 4 days. This study was made possible by development a broadly applicable approach, species-specific transcriptome amplification (SSTA), which enables...
Deafness-causing deficiencies in otoferlin ( OTOF ) have been addressed preclinically using dual adeno-associated virus (AAV)-based approaches. However, timing of transduction, recombination mRNA, and protein expression with hybrid AAV methods not previously characterized. Here, we established an ex vivo assay to determine the kinetics dual-AAV mediated hair cells mouse utricle. We utilized two different recombinant vectors that comprise DB-OTO, one containing 5′ portion under control...
Fusion of mammalian cells to form stable, non-dividing heterokaryons results in nuclear reprogramming without the exchange genetic material. In this report, we show that somatic cell involves activation canonical skeletal muscle transcription factors as well contraction-excitation genes. Thus, effect heterokaryon formation on gene expression is induce a recapitulation differentiation. Heterokaryons formed with relatively refractory type, hepatocyte line HepG2, revealed importance both MyoD...