A5091227018- Proteoglycans and glycosaminoglycans research
- Osteoarthritis Treatment and Mechanisms
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Nerve injury and regeneration
- Periodontal Regeneration and Treatments
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Vascular Procedures and Complications
- Inflammatory mediators and NSAID effects
- Viral Infectious Diseases and Gene Expression in Insects
- Mesenchymal stem cell research
- Neuropeptides and Animal Physiology
- Blood properties and coagulation
- Machine Learning in Bioinformatics
- NF-κB Signaling Pathways
- S100 Proteins and Annexins
- Meta-analysis and systematic reviews
- Exercise and Physiological Responses
- Axon Guidance and Neuronal Signaling
- Renal and related cancers
Case Western Reserve University
2003-2024
University of Kentucky
2011-2020
Clinical Orthopaedics and Related Research
2019
Washington University in St. Louis
2012-2018
University Hospitals of Cleveland
2005-2006
University of Washington
1988-1994
Rush University Medical Center
1988-1991
Texas Health Dallas
1988
Stem cell-based tissue engineering represents a possible alternative for the repair of cartilage defects. Both bone marrow and adipose contain pluripotential cells capable chondrogenesis. This study was qualitative quantitative comparison chondrogenic potential progenitor isolated from aspirates tissue.Bone (BM) matching (AD) overlying posterior superior iliac crest were obtained patients undergoing elective spine surgery. Chondrogenesis induced using an established aggregate culture...
Periosteum contains osteochondral progenitor cells that can differentiate into osteoblasts and chondrocytes during normal bone growth fracture healing. TGF-β1 BMP-2 have been implicated in the regulation of chondrogenic differentiation these cells, but their roles are not fully defined. This study was undertaken to investigate effects on rat periosteum-derived vitro chondrogenesis a three-dimensional aggregate culture. RT-PCR analyses for gene expression cartilage-specific matrix proteins...
Fatigue crack propagation resistance and high-cycle S-N fatigue life of cortical bone allograft tissue are both negatively impacted in a radiation dose-dependent manner from 0 to 25 kGy. The standard sterilization dose 25-35 kGy has been shown induce cleavage collagen molecules into smaller peptides accumulation stable crosslinks within the matrix, suggesting that these mechanisms may influence radiation-induced losses cyclic fracture resistance. objective this study was determine...
We have studied the biosynthesis of cartilage dermatan sulfate proteoglycan II (DS-PGII) (decorin) using in vitro translation mRNA to determine size primary gene product and by radiolabeling protein presence tunicamycin inhibit addition Asn-linked oligosaccharides. Pulse-chase experiments were performed examine post-translational processing secretion. Inhibitors oligosaccharide used whether DS-PGII molecules containing partially processed oligosaccharides could become proteoglycans be...
Abstract Rabbit articular chondrocytes were incubated with recombinant transforming‐growth‐factor‐β 1 , (rhTGF‐β ) and its effect on newly synthesized proteoglycan measured. rhTGF‐β stimulated synthesis at a concentration as low 5 ng/ml without further increase in radiosulfate incorporation up to 50 ng/ml. The quantitative rh‐TGF‐β ‐treated was greater the cell‐associated culture compartment than medium compartment. promoted an increased retention evidenced by t 1/2 from 8 h 11 h. Specific...
Stem cell-based tissue engineering represents a possible alternative for the repair of cartilage defects. Both bone marrow and adipose contain pluripotential cells capable chondrogenesis. This study was qualitative quantitative comparison chondrogenic potential progenitor isolated from aspirates tissue. Bone (BM) matching (AD) overlying posterior superior iliac crest were obtained patients undergoing elective spine surgery. Chondrogenesis induced using an established aggregate culture...
This study describes a new mechanism controlling the production of alternatively spliced isoforms type II procollagen (Col2a1) in vivo. During chondrogenesis, precursor chondrocytes predominantly produce containing exon 2 (type IIA and IID) while Col2a1 mRNA devoid IIB) is major isoform produced by differentiated chondrocytes. We previously identified an additional truncated premature termination codons 6 IIC). transcript utilization another 5' splice site present 2. To determine role this...
The role of proteoglycans in the central nervous system (CNS) is a rapidly evolving field and has major implications CNS injury. Chondroitin sulfate (CSPGs) increase abundance following damage to spinal cord inhibit neurite outgrowth. Major advances understanding interaction between outgrowing neurites CSPGs created need for more robust quantitative analyses further our this interaction. We report use high-throughput assay determine effect various post-translational modifications aggrecan...
Layers of tissue encapsulating vascular prostheses recovered from humans were extracted and analyzed by SDS-polyacrylamide gel electrophoresis to determine the distribution genetically distinct collagen types. Type V was in maximal concentration extracts tissues nearest prosthesis lumen, type III chronically inflamed filling interstices porous prosthesis, I fibrous occlusive or outer capsule tissue. This pattern types across wall may have arisen due influence modulating factors originating...