A5029404338- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA Research and Splicing
- Metabolism, Diabetes, and Cancer
- Medical Research and Treatments
- RNA modifications and cancer
- FOXO transcription factor regulation
- Kruppel-like factors research
- Adipose Tissue and Metabolism
Shanxi Agricultural University
2021-2024
Adipose tissue plays a crucial role in energy metabolism. Several studies have shown that circular RNA (circRNA) is involved the regulation of fat development and lipid However, little known about their involvement adipogenic differentiation ovine stromal vascular fractions (SVFs). Here, based on previous sequencing data bioinformatics analysis, novel circINSR was identified sheep, which acts as sponge to promote miR-152 inhibiting SVFs. The interactions between were examined using...
Fat deposition involves the continuous differentiation of adipocytes and lipid accumulation. Studies have shown that microRNA miR-136 17β-hydroxysteroid dehydrogenase type 12 (HSD17B12) play important roles in However, regulatory mechanism through which targets HSD17B12 during ovine adipogenesis remains unclear. This study aimed to elucidate role their relationship adipose-derived stromal vascular fractions (SVFs). The target between was predicted confirmed using bioinformatics a...
Abstract MicroRNAs (miRNAs) regulate adipogenic differentiation in stromal vascular fractions (SVFs) through post‐transcriptional regulation of transcription factors and other functional genes. miR‐301 the homeobox C8 ( HOXC8 ) gene are involved lipid homeostasis; however, their roles ovine SVFs unknown. Here, we explored effects on regulatory role miR‐301a expression. Additionally, evaluated effect mRNA abundance markers ability to accumulate lipids. We found that regulates by directly...
The functions of tumour protein, translationally controlled 1 (TPT1), have been extensively researched in various cell types. Although TPT1 was reported to accelerating effects on the lipid deposition mouse hepatocytes, and activation roles phosphatidylinositol 3 kinase (PI3K/AKT) pathway, its role differentiation ovine stromal vascular fractions (SVFs) their underlying mechanisms not addressed. Therefore, objective this study explore whether participates adipogenic regulation SVFs...
Fat tissue—a vital energy storage organ—is intricately regulated by various factors, including circular RNA, which plays a significant role in modulating fat development and lipid metabolism. Therefore, this study aims to clarify the regulatory mechanism of sheep adipocyte proliferation differentiation investigating involvement circTIAM1, miR-485-3p, its target gene PLCB1. Through previous sequencing data, circTIAM1 was identified adipocytes, with circularization elucidated, confirming...
旨在揭示miR-33a在绵羊前体脂肪细胞分化中的生物学功能。本研究以15日龄雄性绵羊背部皮下前体脂肪细胞为试验材料，所有的试验均设立3个重复；利用生物信息学软件预测miR-33a的靶基因，并通过双荧光素酶报告试验对预测的潜在靶基因进行验证；用qPCR和Western blotting分别检测miR-33a、Lipin1和IRS2及其编码蛋白的表达，以揭示miR-33a与其靶基因在绵羊前体脂肪细胞分化中的表达规律；慢病毒介导实现miR-33a的过表达和干扰后，检测Lipin1、IRS2和成脂标志基因的表达，并用油红O染色检测脂滴沉积能力，以解析miR-33a对其靶基因的调节机制。生物信息学分析发现，miR-33a与Lipin1和IRS2 3'-UTR都存在结合位点，miR-33a显著下调Lipin1和IRS2野生型双荧光质粒的相对荧光活性（P < 0.05）；在绵羊前体脂肪细胞分化中，miR-33a与Lipin1和IRS2的表达趋势相反；过表达miR-33a后，显著下调了Lipin1（P 0.01）和IRS2（P...
旨在研究miR-128-1-5p对绵羊前体脂肪细胞增殖与分化的调节作用。本研究经理论预测和试验研究验证miR-128-1-5p的靶基因；过表达miR-128-1-5p后，用qPCR检测增殖标志基因的表达，CCK-8和EdU检测细胞增殖情况；用qPCR和Western blotting检测miR-128-1-5p和其靶基因在前体脂肪细胞分化中的表达趋势；通过过表达或抑制miR-128-1-5p研究其对绵羊前体脂肪细胞分化的调节机制；用油红O染色检测成脂能力。结果表明，KLF2是miR-128-1-5p的靶基因。前体脂肪细胞增殖过程中，过表达miR-128-1-5p后，增殖标志基因的表达量显著或极显著降低（P < 0.05或P 0.01），细胞活力显著或极显著降低（P 0.01），新生细胞数显著减少（P 0.05）。前体脂肪细胞分化过程中，miR-128-1-5p与KLF2的表达呈负相关；过表达miR-128-1-5p极显著下调了KLF2 mRNA的表达量（P 0.01），显著下调了KLF2蛋白的表达量（P 0.05），显著或极显著上调了分化标志基因mRNA的表达（P...