A5042348341- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Ion channel regulation and function
- Metabolism and Genetic Disorders
- Photoreceptor and optogenetics research
- Hemoglobin structure and function
- Analytical Chemistry and Chromatography
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- Amino Acid Enzymes and Metabolism
- Click Chemistry and Applications
- Ubiquitin and proteasome pathways
- Carbohydrate Chemistry and Synthesis
University of Oxford
2018-2025
Inner Mongolia University
2025
Nagoya University
2025
Max Planck Institute of Biophysics
2020-2024
China Pharmaceutical University
2024
Guangzhou University
2024
Heilongjiang Academy of Forestry
2024
Capital Medical University
2024
Huazhong University of Science and Technology
2022
Jiangnan University
2011-2021
In neurons, the loading of neurotransmitters into synaptic vesicles uses energy from proton-pumping vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases). These membrane protein complexes possess numerous subunit isoforms, which complicates their analysis. We isolated homogeneous rat brain V-ATPase through its interaction with SidK, a Legionella pneumophila effector protein. Cryo-electron microscopy allowed construction an atomic model, defining enzyme's ATP:proton ratio as 3:10...
Innovating to be nondisruptive Insights into the architecture and stoichiometry of membrane complexes have grown with advances in cryo–electron microscopy native mass spectroscopy. However, most these studies are not context membrane. Chorev et al. released intact directly from lipid vesicles a spectrometer. They analyzed components Escherichia coli inner outer membranes bovine mitochondrial For several identified complexes, they found that differs published results and, some cases,...
Abstract Understanding the dynamics of membrane protein–ligand interactions within a native lipid bilayer is major goal for drug discovery. Typically, cell-based assays are used, however, they often blind to effects protein modifications. In this study, using archetypal G protein-coupled receptor rhodopsin, we found that and its effectors can be released directly from retina rod disc membranes infrared irradiation in mass spectrometer. Subsequent isolation dissociation by multiphoton enabled...
F1Fo ATP synthase functions as a biological rotary generator that makes major contribution to cellular energy production. It comprises two molecular motors coupled together by central and peripheral stalk. Proton flow through the Fo motor generates rotation of stalk, inducing conformational changes in F1 catalyzes Here we present nine cryo-EM structures E. coli 3.1-3.4 Å resolution, four discrete rotational sub-states, which provide comprehensive structural model for this widely studied...
Altered glycosylation patterns of plasma proteins are associated with autoimmune disorders and pathogenesis various cancers. Elucidating glycoprotein microheterogeneity relating subtle changes in the glycan structural repertoire to protein-protein, or protein-small molecule interactions, remains a significant challenge glycobiology. Here, we apply mass spectrometry-based approaches elucidate global site-specific two proteins: α1-acid (AGP) haptoglobin (Hp). We then determine dissociation...
Proton-translocating vacuolar-type ATPases (V-ATPases) are necessary for numerous processes in eukaryotic cells, including receptor-mediated endocytosis, protein maturation, and lysosomal acidification. In mammals, V-ATPase subunit isoforms differentially targeted to various intracellular compartments or tissues, but how these influence enzyme activity is not clear. the yeast Saccharomyces cerevisiae , isoform diversity limited two different versions of proton-translocating a: Vph1p, which...
Abstract G protein-coupled receptors (GPCRs) are cell-surface that respond to various stimuli induce signalling pathways across cell membranes. Recent progress has yielded atomic structures of key intermediates 1,2 and roles for lipids in 3,4 . However, capturing events a wild-type receptor real time, native membrane its downstream effectors, remained elusive. Here we probe the archetypal class A GPCR, rhodopsin, directly from fragments disc membranes using mass spectrometry. We monitor...
Not your typical GPCR Among the large family of G protein–coupled receptors (GPCRs) are many orphans, so called because their signaling reactions remain poorly understood. these is GPR158 which highly expressed in nervous system and implicated processes from cognition to memory mood. Patil et al . determined a high-resolution structure alone bound regulator protein (RGS) complex. has an unusual dimerization mode with extensive interaction interface that locks it conformation likely prevents...
As the most complex post-translational modification, glycosylation involves covalent linking of glycan(s) to a biomolecule. The proteins, peptides, lipids, and nucleic acids results in formation diverse glycoconjugates, like glycoproteins, peptidoglycans, glycopeptides, glycolipids, lipopolysaccharides, glycoRNAs, glycoconjugates are also ubiquitous on surface extracellular vesicles (EVs), cells, bacteria, viruses. conjugated glycans integral cell recognition (e.g., adhesion homing),...
New drugs are urgently needed to combat the global TB epidemic. Targeting simultaneously multiple respiratory enzyme complexes of Mycobacterium tuberculosis is regarded as one most effective treatment options shorten drug administration regimes, and reduce opportunity for emergence resistance. During infection proliferation, cytochrome bd oxidase plays a crucial role mycobacterial pathophysiology by maintaining aerobic respiration at limited oxygen concentrations. Here, we present cryo-EM...
The LAT1-4F2hc complex (SLC7A5-SLC3A2) facilitates uptake of essential amino acids, hormones and drugs. Its dysfunction is associated with many cancers immune/neurological disorders. Here, we apply native mass spectrometry (MS)-based approaches to provide evidence super-dimer formation (LAT1-4F2hc)
A lectin affinity purification-mass spectrometry approach to characterize lectin-reactive glycoproteoforms and elucidate specificities at the intact protein level.
Abstract Conserved lipid transfer proteins of the Ups/PRELI family regulate accumulation in mitochondria by shuttling phospholipids a lipid-specific manner across intermembrane space. Here, we combine structural analysis, unbiased genetic approaches yeast and molecular dynamics simulations to unravel determinants specificity within conserved family. We present structures human PRELID1–TRIAP1 PRELID3b–TRIAP1 complexes, which exert activity for phosphatidic acid phosphatidylserine,...
Integral membrane proteins pose considerable challenges to mass spectrometry (MS) owing the complexity and diversity of components in their native environment. Here, we use MS study post-translational maturation bacteriorhodopsin (bR) archaerhodopsin-3 (AR3), using both octyl-glucoside detergent micelles lipid-based nanoparticles. A lower collision energy was required obtain well-resolved spectra for styrene-maleic acid copolymer (SMA) Lipodisqs than scaffold protein (MSP) Nanodiscs. By...
Introduction The TGF-β signaling pathway is a complex network that plays crucial role in regulating essential biological functions and implicated the onset progression of multiple diseases. This review highlights recent advancements developing inhibitors targeting their potential therapeutic applications various